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A0R083

- GLNA2_MYCS2

UniProt

A0R083 - GLNA2_MYCS2

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Protein

Probable glutamine synthetase 2

Gene
glnA, glnA2, MSMEG_4294, MSMEI_4193
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-4293-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamine synthetase 2 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase 2
Gene namesi
Name:glnA
Synonyms:glnA2
Ordered Locus Names:MSMEG_4294, MSMEI_4193
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Probable glutamine synthetase 2PRO_0000396821Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki363 – 363Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons By similarity.

Protein-protein interaction databases

STRINGi246196.MSMEG_4294.

Structurei

3D structure databases

ProteinModelPortaliA0R083.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000005156.
KOiK01915.
OMAiVHDAAPN.
OrthoDBiEOG6B360N.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R083-1 [UniParc]FASTAAdd to Basket

« Hide

MDRQKEFVLR TLEERDIRFV RLWFTDVLGY LKSVAIAPAE LEGAFEEGIG    50
FDGSSIEGFA RVFESDTVAR PDPSTFQVLP WKTSDGNHYS ARMFCDITMP 100
DGSPSWADSR HVLRRQLAKA SDLGFTCYVH PEIEFFLLKP GPNDGTPPEP 150
ADNGGYFDQA VHDAAPNFRR HAIEALEQMG ISVEFSHHEG APGQQEIDLR 200
YADALSMADN VMTFRYLVKE VALADGVRAS FMPKPFAEHP GSAMHTHMSL 250
FEGDTNAFHS PDDPLQLSDV AKSFIAGILE HANEISAVTN QWVNSYKRLV 300
HGGEAPTAAS WGAANRSALV RVPMYTPHKV SSRRVEVRSP DSACNPYLTF 350
AVLLAAGLRG VEKGYVLGPQ AEDNVWSLTQ EERRAMGYRE LPTSLGNALE 400
SMENSELVAE ALGEHVFDYF LRNKRSEWEN YRSHVTPYEL KNYLSL 446
Length:446
Mass (Da):49,825
Last modified:January 9, 2007 - v1
Checksum:i66EBB5992FAFBB96
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK76204.1.
CP001663 Genomic DNA. Translation: AFP40650.1.
RefSeqiWP_011729709.1. NC_018289.1.
YP_006568945.1. NC_018289.1.
YP_888571.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK76204; ABK76204; MSMEG_4294.
AFP40650; AFP40650; MSMEI_4193.
GeneIDi4533434.
KEGGimsg:MSMEI_4193.
msm:MSMEG_4294.
PATRICi18080945. VBIMycSme59918_4214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK76204.1 .
CP001663 Genomic DNA. Translation: AFP40650.1 .
RefSeqi WP_011729709.1. NC_018289.1.
YP_006568945.1. NC_018289.1.
YP_888571.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0R083.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_4294.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK76204 ; ABK76204 ; MSMEG_4294 .
AFP40650 ; AFP40650 ; MSMEI_4193 .
GeneIDi 4533434.
KEGGi msg:MSMEI_4193.
msm:MSMEG_4294.
PATRICi 18080945. VBIMycSme59918_4214.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000005156.
KOi K01915.
OMAi VHDAAPN.
OrthoDBi EOG6B360N.

Enzyme and pathway databases

BioCyci MSME246196:GJ4Y-4293-MONOMER.

Family and domain databases

Gene3Di 3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
TIGRFAMsi TIGR00653. GlnA. 1 hit.
PROSITEi PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. Cited for: PUPYLATION AT LYS-363, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiGLNA2_MYCS2
AccessioniPrimary (citable) accession number: A0R083
Secondary accession number(s): I7FGW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: September 3, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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