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A0R083 (GLNA2_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutamine synthetase 2

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase 2
Gene names
Name:glnA
Synonyms:glnA2
Ordered Locus Names:MSMEG_4294, MSMEI_4193
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Oligomer of 12 subunits arranged in the form of two hexagons By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   PTMIsopeptide bond
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Probable glutamine synthetase 2
PRO_0000396821

Amino acid modifications

Cross-link363Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sequences

Sequence LengthMass (Da)Tools
A0R083 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 66EBB5992FAFBB96

FASTA44649,825
        10         20         30         40         50         60 
MDRQKEFVLR TLEERDIRFV RLWFTDVLGY LKSVAIAPAE LEGAFEEGIG FDGSSIEGFA 

        70         80         90        100        110        120 
RVFESDTVAR PDPSTFQVLP WKTSDGNHYS ARMFCDITMP DGSPSWADSR HVLRRQLAKA 

       130        140        150        160        170        180 
SDLGFTCYVH PEIEFFLLKP GPNDGTPPEP ADNGGYFDQA VHDAAPNFRR HAIEALEQMG 

       190        200        210        220        230        240 
ISVEFSHHEG APGQQEIDLR YADALSMADN VMTFRYLVKE VALADGVRAS FMPKPFAEHP 

       250        260        270        280        290        300 
GSAMHTHMSL FEGDTNAFHS PDDPLQLSDV AKSFIAGILE HANEISAVTN QWVNSYKRLV 

       310        320        330        340        350        360 
HGGEAPTAAS WGAANRSALV RVPMYTPHKV SSRRVEVRSP DSACNPYLTF AVLLAAGLRG 

       370        380        390        400        410        420 
VEKGYVLGPQ AEDNVWSLTQ EERRAMGYRE LPTSLGNALE SMENSELVAE ALGEHVFDYF 

       430        440 
LRNKRSEWEN YRSHVTPYEL KNYLSL 

« Hide

References

« Hide 'large scale' references
[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[2]"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[3]"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[4]"Expansion of the mycobacterial 'PUPylome'."
Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V., Barry C.E. III, Bark S., Dorrestein P.C.
Mol. Biosyst. 6:376-385(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PUPYLATION AT LYS-363, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000480 Genomic DNA. Translation: ABK76204.1.
CP001663 Genomic DNA. Translation: AFP40650.1.
RefSeqYP_006568945.1. NC_018289.1.
YP_888571.1. NC_008596.1.

3D structure databases

ProteinModelPortalA0R083.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246196.MSMEG_4294.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK76204; ABK76204; MSMEG_4294.
AFP40650; AFP40650; MSMEI_4193.
GeneID13430709.
4533434.
KEGGmsg:MSMEI_4193.
msm:MSMEG_4294.
PATRIC18080945. VBIMycSme59918_4214.

Phylogenomic databases

eggNOGCOG0174.
HOGENOMHOG000005156.
KOK01915.
OMAAEDDVWS.
OrthoDBEOG6B360N.
ProtClustDBCLSK2778365.

Enzyme and pathway databases

BioCycMSME246196:GJ4Y-4293-MONOMER.

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
TIGRFAMsTIGR00653. GlnA. 1 hit.
PROSITEPS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA2_MYCS2
AccessionPrimary (citable) accession number: A0R083
Secondary accession number(s): I7FGW9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: February 19, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families