A0R083 (GLNA2_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamine synthetase 2
EC=6.3.1.2

Alternative name(s):
Glutamate--ammonia ligase 2

Gene names

Name:	glnA
Synonyms:	glnA2
Ordered Locus Names:	MSMEG_4294, MSMEI_4193

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Subunit structure	Oligomer of 12 subunits arranged in the form of two hexagons By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro nitrogen fixation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Probable glutamine synthetase 2		PRO_0000396821
Amino acid modifications
	Cross-link	363		Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sequences

	Sequence		Length	Mass (Da)
	A0R083 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 66EBB5992FAFBB96	FASTA	446	49,825
	10 20 30 40 50 60 MDRQKEFVLR TLEERDIRFV RLWFTDVLGY LKSVAIAPAE LEGAFEEGIG FDGSSIEGFA 70 80 90 100 110 120 RVFESDTVAR PDPSTFQVLP WKTSDGNHYS ARMFCDITMP DGSPSWADSR HVLRRQLAKA 130 140 150 160 170 180 SDLGFTCYVH PEIEFFLLKP GPNDGTPPEP ADNGGYFDQA VHDAAPNFRR HAIEALEQMG 190 200 210 220 230 240 ISVEFSHHEG APGQQEIDLR YADALSMADN VMTFRYLVKE VALADGVRAS FMPKPFAEHP 250 260 270 280 290 300 GSAMHTHMSL FEGDTNAFHS PDDPLQLSDV AKSFIAGILE HANEISAVTN QWVNSYKRLV 310 320 330 340 350 360 HGGEAPTAAS WGAANRSALV RVPMYTPHKV SSRRVEVRSP DSACNPYLTF AVLLAAGLRG 370 380 390 400 410 420 VEKGYVLGPQ AEDNVWSLTQ EERRAMGYRE LPTSLGNALE SMENSELVAE ALGEHVFDYF 430 440 LRNKRSEWEN YRSHVTPYEL KNYLSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Expansion of the mycobacterial 'PUPylome'." Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V., Barry C.E. III, Bark S., Dorrestein P.C. Mol. Biosyst. 6:376-385(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PUPYLATION AT LYS-363, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK76204.1. CP001663 Genomic DNA. Translation: AFP40650.1.
RefSeq	YP_006568945.1. NC_018289.1. YP_888571.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R083.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_4294.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK76204; ABK76204; MSMEG_4294.
GeneID	13430709. 4533434.
KEGG	msg:MSMEI_4193. msm:MSMEG_4294.
PATRIC	18080945. VBIMycSme59918_4214.
Phylogenomic databases
eggNOG	COG0174.
HOGENOM	HOG000005156.
KO	K01915.
OMA	MFRTQVH.
ProtClustDB	CLSK2778365.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-4293-MONOMER.
Family and domain databases
Gene3D	3.10.20.70. 1 hit. 3.30.590.10. 1 hit.
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat_dom. IPR008146. Gln_synth_cat_dom. IPR027303. Gln_synth_gly_rich_site. IPR004809. Gln_synth_I. [Graphical view]
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
SUPFAM	SSF54368. Gln_synt_beta. 1 hit.
TIGRFAMs	TIGR00653. GlnA. 1 hit.
PROSITE	PS00180. GLNA_1. False negative. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA2_MYCS2

Accession

Primary (citable) accession number: A0R083
Secondary accession number(s): I7FGW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program