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A0R083

- GLNA2_MYCS2

UniProt

A0R083 - GLNA2_MYCS2

Protein

Probable glutamine synthetase 2

Gene

glnA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-4293-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glutamine synthetase 2 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase 2
    Gene namesi
    Name:glnA
    Synonyms:glnA2
    Ordered Locus Names:MSMEG_4294, MSMEI_4193
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 446446Probable glutamine synthetase 2PRO_0000396821Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki363 – 363Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Interactioni

    Subunit structurei

    Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

    Protein-protein interaction databases

    STRINGi246196.MSMEG_4294.

    Structurei

    3D structure databases

    ProteinModelPortaliA0R083.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000005156.
    KOiK01915.
    OMAiVHDAAPN.
    OrthoDBiEOG6B360N.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR00653. GlnA. 1 hit.
    PROSITEiPS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0R083-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRQKEFVLR TLEERDIRFV RLWFTDVLGY LKSVAIAPAE LEGAFEEGIG    50
    FDGSSIEGFA RVFESDTVAR PDPSTFQVLP WKTSDGNHYS ARMFCDITMP 100
    DGSPSWADSR HVLRRQLAKA SDLGFTCYVH PEIEFFLLKP GPNDGTPPEP 150
    ADNGGYFDQA VHDAAPNFRR HAIEALEQMG ISVEFSHHEG APGQQEIDLR 200
    YADALSMADN VMTFRYLVKE VALADGVRAS FMPKPFAEHP GSAMHTHMSL 250
    FEGDTNAFHS PDDPLQLSDV AKSFIAGILE HANEISAVTN QWVNSYKRLV 300
    HGGEAPTAAS WGAANRSALV RVPMYTPHKV SSRRVEVRSP DSACNPYLTF 350
    AVLLAAGLRG VEKGYVLGPQ AEDNVWSLTQ EERRAMGYRE LPTSLGNALE 400
    SMENSELVAE ALGEHVFDYF LRNKRSEWEN YRSHVTPYEL KNYLSL 446
    Length:446
    Mass (Da):49,825
    Last modified:January 9, 2007 - v1
    Checksum:i66EBB5992FAFBB96
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK76204.1.
    CP001663 Genomic DNA. Translation: AFP40650.1.
    RefSeqiWP_011729709.1. NC_018289.1.
    YP_006568945.1. NC_018289.1.
    YP_888571.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK76204; ABK76204; MSMEG_4294.
    AFP40650; AFP40650; MSMEI_4193.
    GeneIDi4533434.
    KEGGimsg:MSMEI_4193.
    msm:MSMEG_4294.
    PATRICi18080945. VBIMycSme59918_4214.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK76204.1 .
    CP001663 Genomic DNA. Translation: AFP40650.1 .
    RefSeqi WP_011729709.1. NC_018289.1.
    YP_006568945.1. NC_018289.1.
    YP_888571.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0R083.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_4294.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK76204 ; ABK76204 ; MSMEG_4294 .
    AFP40650 ; AFP40650 ; MSMEI_4193 .
    GeneIDi 4533434.
    KEGGi msg:MSMEI_4193.
    msm:MSMEG_4294.
    PATRICi 18080945. VBIMycSme59918_4214.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000005156.
    KOi K01915.
    OMAi VHDAAPN.
    OrthoDBi EOG6B360N.

    Enzyme and pathway databases

    BioCyci MSME246196:GJ4Y-4293-MONOMER.

    Family and domain databases

    Gene3Di 3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    TIGRFAMsi TIGR00653. GlnA. 1 hit.
    PROSITEi PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. Cited for: PUPYLATION AT LYS-363, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiGLNA2_MYCS2
    AccessioniPrimary (citable) accession number: A0R083
    Secondary accession number(s): I7FGW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3