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Protein

Glutamine synthetase

Gene

glnA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism. It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Feedback-inhibited GlnA also interacts with and regulates the activity of the transcriptional regulator TnrA. During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation. Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity. In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei61Important for inhibition by glutamineBy similarity1
Metal bindingi132Magnesium 1By similarity1
Metal bindingi134Magnesium 2By similarity1
Binding sitei184ATPBy similarity1
Metal bindingi189Magnesium 2By similarity1
Metal bindingi196Magnesium 2By similarity1
Binding sitei241L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi245Magnesium 1; via pros nitrogenBy similarity1
Binding sitei249ATPBy similarity1
Binding sitei298L-glutamateBy similarity1
Binding sitei304L-glutamateBy similarity1
Binding sitei316ATPBy similarity1
Binding sitei316L-glutamateBy similarity1
Binding sitei321ATPBy similarity1
Metal bindingi336Magnesium 1By similarity1
Binding sitei338L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi247 – 249ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-4280-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Synonyms:glnA2
Ordered Locus Names:MSMEG_4294, MSMEI_4193
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003968211 – 446Glutamine synthetaseAdd BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki363Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons. In its feedback-inhibited form, interacts with TnrA in order to block its DNA-binding activity.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_4294

Structurei

3D structure databases

ProteinModelPortaliA0R083
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F Bacteria
COG0174 LUCA
HOGENOMiHOG000005156
KOiK01915
OMAiTFYTHPE
OrthoDBiPOG091H02IE

Family and domain databases

Gene3Di3.10.20.70, 1 hit
InterProiView protein in InterPro
IPR008147 Gln_synt_b-grasp
IPR036651 Gln_synt_N
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR008146 Gln_synth_cat_dom
IPR027303 Gln_synth_gly_rich_site
IPR004809 Gln_synth_I
PfamiView protein in Pfam
PF00120 Gln-synt_C, 1 hit
PF03951 Gln-synt_N, 1 hit
SMARTiView protein in SMART
SM01230 Gln-synt_C, 1 hit
SUPFAMiSSF54368 SSF54368, 1 hit
SSF55931 SSF55931, 1 hit
TIGRFAMsiTIGR00653 GlnA, 1 hit
PROSITEiView protein in PROSITE
PS00181 GLNA_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

A0R083-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRQKEFVLR TLEERDIRFV RLWFTDVLGY LKSVAIAPAE LEGAFEEGIG
60 70 80 90 100
FDGSSIEGFA RVFESDTVAR PDPSTFQVLP WKTSDGNHYS ARMFCDITMP
110 120 130 140 150
DGSPSWADSR HVLRRQLAKA SDLGFTCYVH PEIEFFLLKP GPNDGTPPEP
160 170 180 190 200
ADNGGYFDQA VHDAAPNFRR HAIEALEQMG ISVEFSHHEG APGQQEIDLR
210 220 230 240 250
YADALSMADN VMTFRYLVKE VALADGVRAS FMPKPFAEHP GSAMHTHMSL
260 270 280 290 300
FEGDTNAFHS PDDPLQLSDV AKSFIAGILE HANEISAVTN QWVNSYKRLV
310 320 330 340 350
HGGEAPTAAS WGAANRSALV RVPMYTPHKV SSRRVEVRSP DSACNPYLTF
360 370 380 390 400
AVLLAAGLRG VEKGYVLGPQ AEDNVWSLTQ EERRAMGYRE LPTSLGNALE
410 420 430 440
SMENSELVAE ALGEHVFDYF LRNKRSEWEN YRSHVTPYEL KNYLSL
Length:446
Mass (Da):49,825
Last modified:January 9, 2007 - v1
Checksum:i66EBB5992FAFBB96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK76204.1
CP001663 Genomic DNA Translation: AFP40650.1
RefSeqiWP_011729709.1, NZ_CP009494.1
YP_888571.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK76204; ABK76204; MSMEG_4294
AFP40650; AFP40650; MSMEI_4193
GeneIDi4533434
KEGGimsb:LJ00_21285
msg:MSMEI_4193
msm:MSMEG_4294
PATRICifig|246196.19.peg.4214

Similar proteinsi

Entry informationi

Entry nameiGLN1A_MYCS2
AccessioniPrimary (citable) accession number: A0R083
Secondary accession number(s): I7FGW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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