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Protein

Glutamine synthetase

Gene

glnA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism. It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Feedback-inhibited GlnA also interacts with and regulates the activity of the transcriptional regulator TnrA. During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation. Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity. In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei61Important for inhibition by glutamineBy similarity1
Metal bindingi132Magnesium 1By similarity1
Metal bindingi134Magnesium 2By similarity1
Binding sitei184ATPBy similarity1
Metal bindingi189Magnesium 2By similarity1
Metal bindingi196Magnesium 2By similarity1
Binding sitei241L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi245Magnesium 1; via pros nitrogenBy similarity1
Binding sitei249ATPBy similarity1
Binding sitei298L-glutamateBy similarity1
Binding sitei304L-glutamateBy similarity1
Binding sitei316ATPBy similarity1
Binding sitei316L-glutamateBy similarity1
Binding sitei321ATPBy similarity1
Metal bindingi336Magnesium 1By similarity1
Binding sitei338L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi247 – 249ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Synonyms:glnA2
Ordered Locus Names:MSMEG_4294, MSMEI_4193
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003968211 – 446Glutamine synthetaseAdd BLAST446

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki363Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons. In its feedback-inhibited form, interacts with TnrA in order to block its DNA-binding activity.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_4294.

Structurei

3D structure databases

ProteinModelPortaliA0R083.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.
HOGENOMiHOG000005156.
KOiK01915.
OMAiTFYTHPE.
OrthoDBiPOG091H02IE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R083-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRQKEFVLR TLEERDIRFV RLWFTDVLGY LKSVAIAPAE LEGAFEEGIG
60 70 80 90 100
FDGSSIEGFA RVFESDTVAR PDPSTFQVLP WKTSDGNHYS ARMFCDITMP
110 120 130 140 150
DGSPSWADSR HVLRRQLAKA SDLGFTCYVH PEIEFFLLKP GPNDGTPPEP
160 170 180 190 200
ADNGGYFDQA VHDAAPNFRR HAIEALEQMG ISVEFSHHEG APGQQEIDLR
210 220 230 240 250
YADALSMADN VMTFRYLVKE VALADGVRAS FMPKPFAEHP GSAMHTHMSL
260 270 280 290 300
FEGDTNAFHS PDDPLQLSDV AKSFIAGILE HANEISAVTN QWVNSYKRLV
310 320 330 340 350
HGGEAPTAAS WGAANRSALV RVPMYTPHKV SSRRVEVRSP DSACNPYLTF
360 370 380 390 400
AVLLAAGLRG VEKGYVLGPQ AEDNVWSLTQ EERRAMGYRE LPTSLGNALE
410 420 430 440
SMENSELVAE ALGEHVFDYF LRNKRSEWEN YRSHVTPYEL KNYLSL
Length:446
Mass (Da):49,825
Last modified:January 9, 2007 - v1
Checksum:i66EBB5992FAFBB96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK76204.1.
CP001663 Genomic DNA. Translation: AFP40650.1.
RefSeqiWP_011729709.1. NZ_CP009494.1.
YP_888571.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK76204; ABK76204; MSMEG_4294.
AFP40650; AFP40650; MSMEI_4193.
GeneIDi4533434.
KEGGimsb:LJ00_21285.
msg:MSMEI_4193.
msm:MSMEG_4294.
PATRICifig|246196.19.peg.4214.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1A_MYCS2
AccessioniPrimary (citable) accession number: A0R083
Secondary accession number(s): I7FGW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: June 7, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families