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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:MSMEG_4293, MSMEI_4192
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000728471 – 999Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST999

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_4293.

Structurei

3D structure databases

ProteinModelPortaliA0R082.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 493Adenylyl removaseUniRule annotationAdd BLAST493
Regioni498 – 999Adenylyl transferaseUniRule annotationAdd BLAST502

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000243040.
KOiK00982.
OMAiFTVQLLQ.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

A0R082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVRKPATER PRLPSVGRLG LFDPHAPAHL DQLGWNTDDH VELLWSLSRA
60 70 80 90 100
PDADAALLAM VRLADELKDD WDELNRLLLT DRPLRGRLFA VLGSSLALGD
110 120 130 140 150
HLVAHPQSWR LLHGDVRLPS ARELRETFDD AARSVDIERG ASAAIPPLRD
160 170 180 190 200
LYRDRLLVLA ALDVASTVEN EPVLPFVTVS AHLSDLADAA LSAALIVATR
210 220 230 240 250
TVCGDLDPRL AVIAMGKCGA RELNYVSDVD VIFVGEDIEK DGRSDNLATA
260 270 280 290 300
TRVAGEMMRF AGDAFFEVDA ALRPEGKRGQ LVRTLDSHVA YYRRWAKTWE
310 320 330 340 350
FQALLKARPA AGDPELGQAY IDALMPMVWT ACEREDFVPE VQAMRRRVEE
360 370 380 390 400
LVPADVRSRE IKLGTGGLRD VEFAVQLLQL VHGRTDESLH VASTVDALAA
410 420 430 440 450
LGEGGYVGRD DAANMTASYE FLRLLEHRLQ LQRLKRTHML PDDNDDEAYR
460 470 480 490 500
WLARAAHIRP DGTHDAQGVL REELKRQSLR VSRLHAKLFY QPLLESVGHT
510 520 530 540 550
ALGIGEGMSA EAAERQLAAL GYERPQSALA HLAALTGATG RKGRIQQILL
560 570 580 590 600
PTLLDWLSDT PDPDAGLLAY RRLSDEHSDL RWFLGTLRDE GAVAKRLMRV
610 620 630 640 650
LGTSAYIPEL LMRAPEVIQM YADGPSGPKL LEDDRESRAR ALVASASRYA
660 670 680 690 700
DPVRAIAAAR TLRRRELARI ASADVLGMLD VIDVCKSLTA VWVAVLQAAL
710 720 730 740 750
DVVIRANTPD SGVPARIAVI GMGRLGGGEL GYGSDADVMF VCEPNSGVEE
760 770 780 790 800
SVAVRWSVGV AEQVRALLGT PSADPPLEVD TGLRPEGRSG PLVRTLASYD
810 820 830 840 850
AYYSQWAQPW EIQALLRAHR VAGDLELGER FLLMADKIRY PEGGVSASAV
860 870 880 890 900
QEIRRIKARV DAERLPRGAD PNTHTKLGRG GLADIEWTVQ LLQLRYAHKV
910 920 930 940 950
PALHNTSTLE ALNAIGAAEL VAEGDVELLR EAWLTATRAR NALVLVRGKP
960 970 980 990
TDQLPGPGRQ LNAVALAAGW GSDDGGEFLD NYLRVTRRAK AVVRKIFGG
Length:999
Mass (Da):109,400
Last modified:January 9, 2007 - v1
Checksum:iFA89762C1BCE383B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72782.1.
CP001663 Genomic DNA. Translation: AFP40649.1.
RefSeqiWP_011729708.1. NZ_CP009494.1.
YP_888570.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK72782; ABK72782; MSMEG_4293.
AFP40649; AFP40649; MSMEI_4192.
GeneIDi4532101.
KEGGimsg:MSMEI_4192.
msm:MSMEG_4293.
PATRICifig|246196.19.peg.4213.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLNE_MYCS2
AccessioniPrimary (citable) accession number: A0R082
Secondary accession number(s): I7GD80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 9, 2007
Last modified: July 5, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families