Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D-aminoacyl-tRNA deacylase

Gene

dtd

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.UniRule annotation

Catalytic activityi

Glycyl-tRNA(Ala) + H2O = glycine + tRNA(Ala).UniRule annotation
A D-aminoacyl-tRNA + H2O = a D-amino acid + tRNA.UniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-4277-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
D-aminoacyl-tRNA deacylaseUniRule annotation (EC:3.1.1.96UniRule annotation)
Short name:
DTDUniRule annotation
Alternative name(s):
Gly-tRNA(Ala) deacylaseUniRule annotation (EC:3.1.1.-UniRule annotation)
Gene namesi
Name:dtdUniRule annotation
Ordered Locus Names:MSMEG_4291, MSMEI_4190
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000508561 – 143D-aminoacyl-tRNA deacylaseAdd BLAST143

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_4291

Structurei

3D structure databases

ProteinModelPortaliA0R080
SMRiA0R080
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi135 – 136Gly-cisPro motif, important for rejection of L-amino acidsUniRule annotation2

Domaini

A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.UniRule annotation

Sequence similaritiesi

Belongs to the DTD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108YYA Bacteria
COG1490 LUCA
HOGENOMiHOG000113982
KOiK07560
OMAiGVFQAHM
OrthoDBiPOG091H02D0

Family and domain databases

CDDicd00563 Dtyr_deacylase, 1 hit
Gene3Di3.50.80.10, 1 hit
HAMAPiMF_00518 Deacylase_Dtd, 1 hit
InterProiView protein in InterPro
IPR003732 Daa-tRNA_deacyls_DTD
IPR023509 DTD-like_sf
PANTHERiPTHR10472 PTHR10472, 1 hit
PfamiView protein in Pfam
PF02580 Tyr_Deacylase, 1 hit
SUPFAMiSSF69500 SSF69500, 1 hit
TIGRFAMsiTIGR00256 TIGR00256, 1 hit

Sequencei

Sequence statusi: Complete.

A0R080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLVQRVSR AQVTVDGEVV GAIDPQPQGL LALVGVTHDD DAAKAQRLAE
60 70 80 90 100
KLWKLRILDD ERSASDVAAP ILVISQFTLY ANTDKGRRPS WNAAAPGSVA
110 120 130 140
EPLVTEFTEA LRRLGATVET GVFGAHMEVE LVNDGPVTVL LEL
Length:143
Mass (Da):15,382
Last modified:January 9, 2007 - v1
Checksum:iA5F492118978D1D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK74712.1
CP001663 Genomic DNA Translation: AFP40647.1
RefSeqiWP_011729707.1, NZ_CP009494.1
YP_888568.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK74712; ABK74712; MSMEG_4291
AFP40647; AFP40647; MSMEI_4190
GeneIDi4534728
KEGGimsb:LJ00_21270
msg:MSMEI_4190
msm:MSMEG_4291
PATRICifig|246196.19.peg.4211

Similar proteinsi

Entry informationi

Entry nameiDTD_MYCS2
AccessioniPrimary (citable) accession number: A0R080
Secondary accession number(s): I7G4T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health