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Protein

D-aminoacyl-tRNA deacylase

Gene

dtd

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.UniRule annotation

Catalytic activityi

Glycyl-tRNA(Ala) + H2O = glycine + tRNA(Ala).UniRule annotation
A D-aminoacyl-tRNA + H2O = a D-amino acid + tRNA.UniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
D-aminoacyl-tRNA deacylaseUniRule annotation (EC:3.1.1.96UniRule annotation)
Short name:
DTDUniRule annotation
Alternative name(s):
Gly-tRNA(Ala) deacylaseUniRule annotation (EC:3.1.1.-UniRule annotation)
Gene namesi
Name:dtdUniRule annotation
Ordered Locus Names:MSMEG_4291, MSMEI_4190
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000508561 – 143D-aminoacyl-tRNA deacylaseAdd BLAST143

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_4291.

Structurei

3D structure databases

ProteinModelPortaliA0R080.
SMRiA0R080.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi135 – 136Gly-cisPro motif, important for rejection of L-amino acidsUniRule annotation2

Domaini

A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.UniRule annotation

Sequence similaritiesi

Belongs to the DTD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108YYA. Bacteria.
COG1490. LUCA.
HOGENOMiHOG000113982.
KOiK07560.
OMAiDGPVTIW.
OrthoDBiPOG091H02D0.

Family and domain databases

CDDicd00563. Dtyr_deacylase. 1 hit.
Gene3Di3.50.80.10. 1 hit.
HAMAPiMF_00518. Deacylase_Dtd. 1 hit.
InterProiView protein in InterPro
IPR003732. Daa-tRNA_deacyls_DTD.
IPR023509. DTD-like_dom_sf.
PANTHERiPTHR10472. PTHR10472. 1 hit.
PfamiView protein in Pfam
PF02580. Tyr_Deacylase. 1 hit.
SUPFAMiSSF69500. SSF69500. 1 hit.
TIGRFAMsiTIGR00256. TIGR00256. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLVQRVSR AQVTVDGEVV GAIDPQPQGL LALVGVTHDD DAAKAQRLAE
60 70 80 90 100
KLWKLRILDD ERSASDVAAP ILVISQFTLY ANTDKGRRPS WNAAAPGSVA
110 120 130 140
EPLVTEFTEA LRRLGATVET GVFGAHMEVE LVNDGPVTVL LEL
Length:143
Mass (Da):15,382
Last modified:January 9, 2007 - v1
Checksum:iA5F492118978D1D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74712.1.
CP001663 Genomic DNA. Translation: AFP40647.1.
RefSeqiWP_011729707.1. NZ_CP009494.1.
YP_888568.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74712; ABK74712; MSMEG_4291.
AFP40647; AFP40647; MSMEI_4190.
GeneIDi4534728.
KEGGimsb:LJ00_21270.
msg:MSMEI_4190.
msm:MSMEG_4291.
PATRICifig|246196.19.peg.4211.

Similar proteinsi

Entry informationi

Entry nameiDTD_MYCS2
AccessioniPrimary (citable) accession number: A0R080
Secondary accession number(s): I7G4T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: November 22, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families