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Protein

GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase

Gene

pimB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM) (PubMed:10531370, PubMed:19638342). Catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (Ac1PIM2) (PubMed:19638342). PimB also catalyzes the addition of a mannosyl residue from GDP-Man to the position 6 of phosphatidyl-myo-inositol bearing an acylated alpha-1,2-linked mannose residue (Ac1PIM1) to generate monoacylated phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (Ac1PIM2) (PubMed:19638342). The addition of the second mannosyl residue by PimB preferentially occurs before the acylation of the mannosyl residue transferred by PimA. Also able to transfer a mannosyl residue from GDP-Man to the position 6 of a phosphatidyl-myo-inositol (PI), but this reaction is very slow (PubMed:19638342).2 Publications

Catalytic activityi

Transfers one or more alpha-D-mannose residues from GDP-mannose to positions 2,6 and others in 1-phosphatidyl-myo-inositol.1 Publication

Pathwayi: phosphatidylinositol metabolism

This protein is involved in the pathway phosphatidylinositol metabolism, which is part of Phospholipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway phosphatidylinositol metabolism and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei200GDP-mannoseBy similarity1
Binding sitei205GDP-mannoseBy similarity1
Binding sitei257GDP-mannose; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei294GDP-mannoseBy similarity1

GO - Molecular functioni

  • glycolipid 6-alpha-mannosyltransferase activity Source: UniProtKB
  • phosphatidylinositol alpha-mannosyltransferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Virulence

Enzyme and pathway databases

BRENDAi2.4.1.57. 3512.
UniPathwayiUPA00949.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase1 Publication (EC:2.4.1.571 Publication)
Alternative name(s):
Alpha-D-mannose-alpha-(1-6)-phosphatidylmyo-inositol-mannosyltransferase1 Publication
Alpha-mannosyltransferase1 Publication
Short name:
Alpha-ManT1 Publication
Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferaseCurated
Phosphatidylinositol alpha-mannosyltransferase1 Publication
Short name:
PI alpha-mannosyltransferase1 Publication
Gene namesi
Name:pimB1 Publication
Ordered Locus Names:MSMEG_4253, MSMEI_4154
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003937351 – 382GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferaseAdd BLAST382

Proteomic databases

PRIDEiA0R043.

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_4253.

Structurei

3D structure databases

ProteinModelPortaliA0R043.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105JNK. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077286.
KOiK13668.
OMAiYAMSDIF.
OrthoDBiPOG091H0AO3.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0R043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLVTNDFPP RRGGIQSYLE AFVGELVRTH ELTVYAPKWK GAEEYDEKAA
60 70 80 90 100
RSGYRVVRHP TTLMLPEPTV ASRMKRLIGE HDIETVWFGA AAPLALLGPL
110 120 130 140 150
ARRAGARRIV ASTHGHEVGW SMLPVARTAL RRIGNDADVV TFVSRYTRSR
160 170 180 190 200
FASAFGPSAA LEHLPPGVDT DRFAPDPDAR ARMRERYGLG DRPVVVCLSR
210 220 230 240 250
LVPRKGQDML IRALPELRRR VPDTALAIVG GGPYLETLQR MASDLGVAEH
260 270 280 290 300
VVFTRGIPAE ELPAHHAMAD VFAMPCRTRG AGLDVEGLGI VYLEASACGV
310 320 330 340 350
PVVAGRSGGA PETVLDGKTG TVVDGTDVDA ITTAVGDLLA DPRRAAAMGV
360 370 380
AGRHWALDNW QWRTRGARLA ELLSGRREAR QA
Length:382
Mass (Da):41,407
Last modified:January 9, 2007 - v1
Checksum:i938A199444B1BA4D
GO

Sequence cautioni

The sequence AFP40611 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74170.1.
CP001663 Genomic DNA. Translation: AFP40611.1. Different initiation.
RefSeqiWP_011729679.1. NC_008596.1.
YP_888531.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74170; ABK74170; MSMEG_4253.
AFP40611; AFP40611; MSMEI_4154.
GeneIDi4536134.
KEGGimsg:MSMEI_4154.
msm:MSMEG_4253.
PATRICi18080865. VBIMycSme59918_4174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74170.1.
CP001663 Genomic DNA. Translation: AFP40611.1. Different initiation.
RefSeqiWP_011729679.1. NC_008596.1.
YP_888531.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R043.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_4253.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

PRIDEiA0R043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK74170; ABK74170; MSMEG_4253.
AFP40611; AFP40611; MSMEI_4154.
GeneIDi4536134.
KEGGimsg:MSMEI_4154.
msm:MSMEG_4253.
PATRICi18080865. VBIMycSme59918_4174.

Phylogenomic databases

eggNOGiENOG4105JNK. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077286.
KOiK13668.
OMAiYAMSDIF.
OrthoDBiPOG091H0AO3.

Enzyme and pathway databases

UniPathwayiUPA00949.
BRENDAi2.4.1.57. 3512.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIMB_MYCS2
AccessioniPrimary (citable) accession number: A0R043
Secondary accession number(s): I7GBP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: January 9, 2007
Last modified: November 30, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.