A0R024 (RSMH_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	MSMEG_4235

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000386991
Regions
	Region	45 – 47	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	64	1	S-adenosyl-L-methionine By similarity
	Binding site	112	1	S-adenosyl-L-methionine By similarity
	Binding site	119	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0R024 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 02EB55A687BB9073	FASTA	326	35,448
	10 20 30 40 50 60 MTNDPDHGHI PVLLDRCVEL LAPALTRTDP DGTEAVLVDA TLGAGGHSER FLTDFPALRL 70 80 90 100 110 120 IGLDRDPNAL AIAGDRLAPF GDRVTLVRTR YDGITSALAE AGVRRIDGVL FDLGVSSMQL 130 140 150 160 170 180 DQKQRGFSYS ADAPLDMRMD PDALLTAAEI VNTYDAKTLT RILRDYGEER FAGRIADKIV 190 200 210 220 230 240 RRRAKQPFTT TSELVELLYE AIPAPARRTG GHPAKRTFQA LRIAVNAELD SLRAAIPAAL 250 260 270 280 290 300 AALRPGGRIV VMAYQSLEDR IVKQAFSTVT ASRTPPGLPM ELPGHEPEFV AVTRGAERAD 310 320 EQEIERNPRS APVRLRALEK VGGRGS

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK70680.1.
RefSeq	YP_888512.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0R024.
ModBase	Search...
Protein-protein interaction databases
STRING	A0R024.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000043829; EBMYCP00000042166; EBMYCG00000043824.
GeneID	4537686.
GenomeReviews	Gene locus MSMEG_4235 in contig CP000480_GR.
KEGG	msm:MSMEG_4235.
NMPDR	fig|246196.1.peg.4239.
PATRIC	18080827. VBIMycSme59918_4155.
TIGR	MSMEG_4235.
Phylogenomic databases
eggNOG	COG0275.
GeneTree	EBGT00050000016736.
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_4235-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_MYCS2

Accession

Primary (citable) accession number: A0R024

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program