Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UDP-N-acetylmuramoylalanine--D-glutamate ligase

Gene

murD

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi126 – 1327ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-4228-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligaseUniRule annotation (EC:6.3.2.9UniRule annotation)
Alternative name(s):
D-glutamic acid-adding enzymeUniRule annotation
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetaseUniRule annotation
Gene namesi
Name:murDUniRule annotation
Ordered Locus Names:MSMEG_4229, MSMEI_4129
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493UDP-N-acetylmuramoylalanine--D-glutamate ligasePRO_0000301439Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_4229.

Structurei

3D structure databases

ProteinModelPortaliA0R018.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DMZ. Bacteria.
COG0771. LUCA.
HOGENOMiHOG000049429.
KOiK01925.
OMAiPGIPYTN.
OrthoDBiPOG091H002Z.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 2 hits.
HAMAPiMF_00639. MurD. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 2 hits.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01087. murD. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R018-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDVTGDLAP LTPGARVLVT GAGITGRAVL GALAPLEVAA TLCDDNADAL
60 70 80 90 100
AALAAKGVAV IAPGDAIASI GDYALVVTSP GFAPSAPVLA AAAAAGVPIW
110 120 130 140 150
GDVELAWRLD AAGRYGPPRR WLVVTGTNGK TTTTSMLHDM LVAGGRRSVL
160 170 180 190 200
CGNIGDPVLA VLDQPAELLA VELSSFQLHW APSLRPEAGV VLNVAEDHLD
210 220 230 240 250
WHGSMAAYAR DKARVLDGRV AVVGLDDPVA AGLLPTAAAT VRVGFRLGDP
260 270 280 290 300
AAGELGVRDG KLIDRAFDDG VELADCADIG VAGPIGVLDA LGAAALARAV
310 320 330 340 350
GVAPGAIGAA LASFQVGRHR AEVVGTVDGV VFIDDSKATN PHAAQASITA
360 370 380 390 400
YDRVVWVAGG LLKGASVDEL VRQVANRLAG AVLIGRDRQM VADALSRHAP
410 420 430 440 450
DVPVVELVAG EDSGVLGTNE SIESVGDHVT RVIEVGGRPV SDAVMSAVVS
460 470 480 490
AARDLASPGD TVLLAPAGAS FDQFSGYGQR GDAFAAAVRA AVG
Length:493
Mass (Da):49,558
Last modified:January 9, 2007 - v1
Checksum:i88CEA23CC554E771
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72327.1.
CP001663 Genomic DNA. Translation: AFP40586.1.
RefSeqiWP_011729658.1. NZ_CP009494.1.
YP_888506.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK72327; ABK72327; MSMEG_4229.
AFP40586; AFP40586; MSMEI_4129.
GeneIDi4531703.
KEGGimsb:LJ00_20965.
msg:MSMEI_4129.
msm:MSMEG_4229.
PATRICi18080815. VBIMycSme59918_4149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72327.1.
CP001663 Genomic DNA. Translation: AFP40586.1.
RefSeqiWP_011729658.1. NZ_CP009494.1.
YP_888506.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R018.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_4229.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK72327; ABK72327; MSMEG_4229.
AFP40586; AFP40586; MSMEI_4129.
GeneIDi4531703.
KEGGimsb:LJ00_20965.
msg:MSMEI_4129.
msm:MSMEG_4229.
PATRICi18080815. VBIMycSme59918_4149.

Phylogenomic databases

eggNOGiENOG4105DMZ. Bacteria.
COG0771. LUCA.
HOGENOMiHOG000049429.
KOiK01925.
OMAiPGIPYTN.
OrthoDBiPOG091H002Z.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciMSME246196:GJ4Y-4228-MONOMER.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 2 hits.
HAMAPiMF_00639. MurD. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 2 hits.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01087. murD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURD_MYCS2
AccessioniPrimary (citable) accession number: A0R018
Secondary accession number(s): I7GBM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.