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Protein

UDP-N-acetylmuramate--L-alanine ligase

Gene

murC

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi122 – 1287ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-4225-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramate--L-alanine ligaseUniRule annotation (EC:6.3.2.8UniRule annotation)
Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetaseUniRule annotation
Gene namesi
Name:murCUniRule annotation
Ordered Locus Names:MSMEG_4226, MSMEI_4126
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482UDP-N-acetylmuramate--L-alanine ligasePRO_1000004372Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_4226.

Structurei

3D structure databases

ProteinModelPortaliA0R015.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DFU. Bacteria.
COG0773. LUCA.
HOGENOMiHOG000256031.
KOiK01924.
OMAiFHFIGIG.
OrthoDBiEOG64BQ73.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00046. MurC.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR005758. UDP-N-AcMur_Ala_ligase_murC.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01082. murC. 1 hit.

Sequencei

Sequence statusi: Complete.

A0R015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGISLPPEL QRVHMVGIGG AGMSGVARIL LDRGGLVSGS DAKESRGVVA
60 70 80 90 100
LRARGAEIRI GHDASSLDLL PGGPTAVVTT HAAIPKTNPE LVEARKRGIP
110 120 130 140 150
VILRPVVLAK LMTGYTTLMV TGTHGKTTTT SMLIVALQHS GFDPSFAVGG
160 170 180 190 200
ELGEAGTNAH HGSGTTFVAE ADESDGSLLE YTPNVAVVTN IEADHLDFFG
210 220 230 240 250
SEQAYTAVFD SFVERIAPGG ALVVCTDDPG AAALADRTDA LGIRVLRYGS
260 270 280 290 300
TGDNLAGTLL SWEQQGTGAV AHIRLAGEHN PRAVRLSVPG RHMALNALAA
310 320 330 340 350
LLAAREIGAP TDSVLDGLAG FEGVRRRFEL VGTAAGARVF DDYAHHPTEV
360 370 380 390 400
RATLEAARTV VDQNGGRVVV AFQPHLYSRT ATFAHEFGAA LSVADQVVVL
410 420 430 440 450
DVYAAREQPM AGVSGATVAD HVTAPVTYVP DFSAVAAHVA SIARSGDVIL
460 470 480
TMGAGDVTML GGEILSELRL KDNRGMPGAG AS
Length:482
Mass (Da):49,804
Last modified:January 9, 2007 - v1
Checksum:i6759FEC299BA93C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71244.1.
CP001663 Genomic DNA. Translation: AFP40583.1.
RefSeqiWP_011729655.1. NZ_CP009494.1.
YP_888503.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71244; ABK71244; MSMEG_4226.
AFP40583; AFP40583; MSMEI_4126.
GeneIDi4537247.
KEGGimsb:LJ00_20950.
msg:MSMEI_4126.
msm:MSMEG_4226.
PATRICi18080809. VBIMycSme59918_4146.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71244.1.
CP001663 Genomic DNA. Translation: AFP40583.1.
RefSeqiWP_011729655.1. NZ_CP009494.1.
YP_888503.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0R015.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_4226.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71244; ABK71244; MSMEG_4226.
AFP40583; AFP40583; MSMEI_4126.
GeneIDi4537247.
KEGGimsb:LJ00_20950.
msg:MSMEI_4126.
msm:MSMEG_4226.
PATRICi18080809. VBIMycSme59918_4146.

Phylogenomic databases

eggNOGiENOG4105DFU. Bacteria.
COG0773. LUCA.
HOGENOMiHOG000256031.
KOiK01924.
OMAiFHFIGIG.
OrthoDBiEOG64BQ73.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciMSME246196:GJ4Y-4225-MONOMER.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00046. MurC.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR016040. NAD(P)-bd_dom.
IPR005758. UDP-N-AcMur_Ala_ligase_murC.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01082. murC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiMURC_MYCS2
AccessioniPrimary (citable) accession number: A0R015
Secondary accession number(s): I7FPD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: December 9, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.