A0QZY5 (UPPP_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Undecaprenyl-diphosphatase
EC=3.6.1.27

Alternative name(s):
Bacitracin resistance protein
Undecaprenyl pyrophosphate phosphatase

Gene names

Name:	uppP
Ordered Locus Names:	MSMEG_4194, MSMEI_4096

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin By similarity. HAMAP-Rule MF_01006
Catalytic activity	Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate. HAMAP-Rule MF_01006
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01006.
Miscellaneous	Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available. HAMAP-Rule MF_01006
Sequence similarities	Belongs to the UppP family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dephosphorylation Inferred from electronic annotation. Source: InterPro peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW response to antibiotic Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	undecaprenyl-diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Undecaprenyl-diphosphatase HAMAP-Rule MF_01006		PRO_0000303030
Regions
	Transmembrane	1 – 21	21	Helical; Potential
	Transmembrane	39 – 59	21	Helical; Potential
	Transmembrane	84 – 104	21	Helical; Potential
	Transmembrane	115 – 135	21	Helical; Potential
	Transmembrane	159 – 179	21	Helical; Potential
	Transmembrane	188 – 208	21	Helical; Potential
	Transmembrane	222 – 242	21	Helical; Potential
	Transmembrane	253 – 273	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A0QZY5 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 33FCA6A718113887	FASTA	276	29,715
	10 20 30 40 50 60 MSWLQVIVLS IVQGLTEFLP VSSSGHLAIT SQVFFDDDAG ASFTAVTQLG TEFAVLIYFA 70 80 90 100 110 120 KDIGRIIKSW FLGLRAPEHR DADYRLGWFV IVGTIPIGVF GLLFKDEIRT GARNLWLVAT 130 140 150 160 170 180 ALIVFSVVIA AAEYYGRQVR QVEQLTWRDS VIVGLAQCLA LMPGVSRSGA TISAGLFLGL 190 200 210 220 230 240 KREVAARFGF LLAIPAVLAS GLFSLPDAFH PVGEGMSASG PQLIVATVIA FVVGFAAIAW 250 260 270 FLKFLVSHSM YWFVGYRVVL GVVVLALLGT GVLAAQ

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK73321.1. CP001663 Genomic DNA. Translation: AFP40554.1.
RefSeq	YP_006568849.1. NC_018289.1. YP_888473.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QZY5.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_4194.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK73321; ABK73321; MSMEG_4194.
GeneID	13430612. 4531825.
KEGG	msg:MSMEI_4096. msm:MSMEG_4194.
PATRIC	18080745. VBIMycSme59918_4115.
Phylogenomic databases
eggNOG	COG1968.
HOGENOM	HOG000218357.
KO	K06153.
OMA	HLAIFTA.
ProtClustDB	PRK00281.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-4193-MONOMER.
Family and domain databases
HAMAP	MF_01006. Undec_diphosphatase.
InterPro	IPR003824. Bacitracin-R_BacA. [Graphical view]
Pfam	PF02673. BacA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00753. undec_PP_bacA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPPP_MYCS2

Accession

Primary (citable) accession number: A0QZY5
Secondary accession number(s): I7GD14

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program