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A0QZ47

- PSB_MYCS2

UniProt

A0QZ47 - PSB_MYCS2

Protein

Proteasome subunit beta

Gene

prcB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.smegmatis proteasome is able to cleave oligopeptides after hydrophobic residues, thus displaying chymotrypsin-like activity. Among the identified substrates of the M.smegmatis proteasome are the pupylated SodA and Ino1 proteins.2 PublicationsUniRule annotation

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.2 PublicationsUniRule annotation

    Enzyme regulationi

    The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity. Peptidolytic activity is inhibited by N-acetyl-Leu-Leu-norleucinal (Ac-LLnL) in vitro.1 PublicationUniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631NucleophileUniRule annotation

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. modification-dependent protein catabolic process Source: UniProtKB-HAMAP
    2. proteasomal protein catabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-3894-MONOMER.
    UniPathwayiUPA00997.

    Protein family/group databases

    MEROPSiT01.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
    Alternative name(s):
    20S proteasome beta subunitUniRule annotation
    Proteasome core protein PrcBUniRule annotation
    Gene namesi
    Name:prcBUniRule annotation
    Ordered Locus Names:MSMEG_3895, MSMEI_3805
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. proteasome core complex, beta-subunit complex Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cytoplasm, Proteasome

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene are viable and grow at the wild-type rate, but show a large reduction in the rate of degradation of the pupylated substrates.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 6262Removed in mature form; by autocatalysisUniRule annotationPRO_0000383487Add
    BLAST
    Chaini63 – 303241Proteasome subunit betaPRO_0000383488Add
    BLAST

    Keywords - PTMi

    Autocatalytic cleavage, Zymogen

    Interactioni

    Subunit structurei

    The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.UniRule annotation

    Protein-protein interaction databases

    STRINGi246196.MSMEG_3895.

    Structurei

    3D structure databases

    ProteinModelPortaliA0QZ47.
    SMRiA0QZ47. Positions 25-284.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000245308.
    KOiK03433.
    OrthoDBiEOG6XM79W.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_02113_B. Proteasome_B_B.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR022483. Pept_T1A_Psome_suB_actinobac.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR03690. 20S_bact_beta. 1 hit.
    PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A0QZ47-1 [UniParc]FASTAAdd to Basket

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    MTWRDNQSFP QPTLNTTGIP SVPVDLSSFS ELLSRQAPEL LPVNRVAYGT    50
    TPVGPTDAVP HGTTIVALKY PGGVLIAGDR RSTQGNMIAG RDVQKVYITD 100
    DYTATGIAGT AAIAVEFARL YAVELEHYEK LEGVPLTFRG KVNRLAIMVR 150
    GNLGAALQGF VALPLLVGYD LDDPHPEGAG RIVSFDAAGG WNIEEEGYQS 200
    VGSGSIFAKS SMKKLYSQVS DADSALKVAV EALYDAADDD SATGGPDLVR 250
    GIYPTAVTIG AEGAEEVPET RIAELAREVI ESRSRTDTFG PDARRGIDAR 300
    GDS 303
    Length:303
    Mass (Da):32,131
    Last modified:September 22, 2009 - v2
    Checksum:iF464A036F8CD459C
    GO

    Sequence cautioni

    The sequence ABK70234.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF009645 Genomic DNA. Translation: AAC45614.1.
    CP000480 Genomic DNA. Translation: ABK70234.1. Different initiation.
    CP001663 Genomic DNA. Translation: AFP40263.1.
    RefSeqiWP_014877854.1. NC_018289.1.
    YP_006568558.1. NC_018289.1.
    YP_888185.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK70234; ABK70234; MSMEG_3895.
    AFP40263; AFP40263; MSMEI_3805.
    GeneIDi4534165.
    KEGGimsg:MSMEI_3805.
    msm:MSMEG_3895.
    PATRICi18080185. VBIMycSme59918_3835.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF009645 Genomic DNA. Translation: AAC45614.1 .
    CP000480 Genomic DNA. Translation: ABK70234.1 . Different initiation.
    CP001663 Genomic DNA. Translation: AFP40263.1 .
    RefSeqi WP_014877854.1. NC_018289.1.
    YP_006568558.1. NC_018289.1.
    YP_888185.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0QZ47.
    SMRi A0QZ47. Positions 25-284.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_3895.

    Protein family/group databases

    MEROPSi T01.005.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK70234 ; ABK70234 ; MSMEG_3895 .
    AFP40263 ; AFP40263 ; MSMEI_3805 .
    GeneIDi 4534165.
    KEGGi msg:MSMEI_3805.
    msm:MSMEG_3895.
    PATRICi 18080185. VBIMycSme59918_3835.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000245308.
    KOi K03433.
    OrthoDBi EOG6XM79W.

    Enzyme and pathway databases

    UniPathwayi UPA00997 .
    BioCyci MSME246196:GJ4Y-3894-MONOMER.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_02113_B. Proteasome_B_B.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR022483. Pept_T1A_Psome_suB_actinobac.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR03690. 20S_bact_beta. 1 hit.
    PROSITEi PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Inactivation of the 20S proteasome in Mycobacterium smegmatis."
      Knipfer N., Shrader T.E.
      Mol. Microbiol. 25:375-383(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, DISRUPTION PHENOTYPE.
    2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    5. "Proteasomal protein degradation in mycobacteria is dependent upon a prokaryotic ubiquitin-like protein."
      Burns K.E., Liu W.-T., Boshoff H.I.M., Dorrestein P.C., Barry C.E. III
      J. Biol. Chem. 284:3069-3075(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, PROTEIN SUBSTRATES, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPSB_MYCS2
    AccessioniPrimary (citable) accession number: A0QZ47
    Secondary accession number(s): I7FNI5, O30518
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 64 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3