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A0QZ46

- PSA_MYCS2

UniProt

A0QZ46 - PSA_MYCS2

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Protein
Proteasome subunit alpha
Gene
prcA, MSMEG_3894, MSMEI_3804
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.smegmatis proteasome is able to cleave oligopeptides after hydrophobic residues, thus displaying chymotrypsin-like activity. Identified substrates of the M.smegmatis proteasome are the pupylated SodA and Ino1 proteins.2 Publications

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.2 Publications

Enzyme regulationi

The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity. Peptidolytic activity is inhibited by N-acetyl-Leu-Leu-norleucinal (Ac-LLnL) in vitro.1 Publication

Pathwayi

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3893-MONOMER.
UniPathwayiUPA00997.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit
Proteasome core protein PrcA
Gene namesi
Name:prcA
Ordered Locus Names:MSMEG_3894, MSMEI_3804
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. proteasome core complex, alpha-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are viable and grow at the wild-type rate, but show a large reduction in the rate of degradation of the pupylated substrates.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246Proteasome subunit alphaUniRule annotation
PRO_0000383483Add
BLAST

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC By similarity.

Protein-protein interaction databases

STRINGi246196.MSMEG_3894.

Structurei

3D structure databases

ProteinModelPortaliA0QZ46.
SMRiA0QZ46. Positions 8-234.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000245319.
KOiK03432.
OMAiGRYNEFE.
OrthoDBiEOG6NKQZG.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00289_B. Proteasome_A_B.
InterProiIPR029055. Ntn_hydrolases_N.
IPR022296. Proteasome_asu_bac.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03691. 20S_bact_alpha. 1 hit.
PROSITEiPS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QZ46-1 [UniParc]FASTAAdd to Basket

« Hide

MSFPYFISPE QAMRERSELA RKGIARGRSV VALAYSEGVL FVAENPSRSL    50
QKVSELYDRV GFAAVGRFNE FDNLRRGGIQ FADTRGYAYD RRDVTGRQLA 100
NVYAQTLGTI FTEQAKPYEV ELCVAEVAHY GETKAPELYR ITYDGSIADE 150
PHFVVMGGTT EPIIAALNES YTENASLQDA VEIAVKALSA SAEGAEPRSL 200
GPSTLEVAIL DAGRPRRAFR RITGAALEAL LPEQPQQADS GDKPTE 246
Length:246
Mass (Da):26,915
Last modified:January 9, 2007 - v1
Checksum:i7869013FEFD30231
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF009645 Genomic DNA. Translation: AAC45615.1.
CP000480 Genomic DNA. Translation: ABK70795.1.
CP001663 Genomic DNA. Translation: AFP40262.1.
RefSeqiYP_006568557.1. NC_018289.1.
YP_888184.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70795; ABK70795; MSMEG_3894.
AFP40262; AFP40262; MSMEI_3804.
GeneIDi4535010.
KEGGimsg:MSMEI_3804.
msm:MSMEG_3894.
PATRICi18080183. VBIMycSme59918_3834.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF009645 Genomic DNA. Translation: AAC45615.1 .
CP000480 Genomic DNA. Translation: ABK70795.1 .
CP001663 Genomic DNA. Translation: AFP40262.1 .
RefSeqi YP_006568557.1. NC_018289.1.
YP_888184.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0QZ46.
SMRi A0QZ46. Positions 8-234.
ModBasei Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_3894.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK70795 ; ABK70795 ; MSMEG_3894 .
AFP40262 ; AFP40262 ; MSMEI_3804 .
GeneIDi 4535010.
KEGGi msg:MSMEI_3804.
msm:MSMEG_3894.
PATRICi 18080183. VBIMycSme59918_3834.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000245319.
KOi K03432.
OMAi GRYNEFE.
OrthoDBi EOG6NKQZG.

Enzyme and pathway databases

UniPathwayi UPA00997 .
BioCyci MSME246196:GJ4Y-3893-MONOMER.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_00289_B. Proteasome_A_B.
InterProi IPR029055. Ntn_hydrolases_N.
IPR022296. Proteasome_asu_bac.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR03691. 20S_bact_alpha. 1 hit.
PROSITEi PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Inactivation of the 20S proteasome in Mycobacterium smegmatis."
    Knipfer N., Shrader T.E.
    Mol. Microbiol. 25:375-383(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, DISRUPTION PHENOTYPE.
  2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  5. "Proteasomal protein degradation in mycobacteria is dependent upon a prokaryotic ubiquitin-like protein."
    Burns K.E., Liu W.-T., Boshoff H.I.M., Dorrestein P.C., Barry C.E. III
    J. Biol. Chem. 284:3069-3075(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PROTEIN SUBSTRATES, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPSA_MYCS2
AccessioniPrimary (citable) accession number: A0QZ46
Secondary accession number(s): I7G3V1, O30519
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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