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A0QZ46

- PSA_MYCS2

UniProt

A0QZ46 - PSA_MYCS2

Protein

Proteasome subunit alpha

Gene

prcA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.smegmatis proteasome is able to cleave oligopeptides after hydrophobic residues, thus displaying chymotrypsin-like activity. Identified substrates of the M.smegmatis proteasome are the pupylated SodA and Ino1 proteins.2 PublicationsUniRule annotation

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.2 PublicationsUniRule annotation

    Enzyme regulationi

    The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity. Peptidolytic activity is inhibited by N-acetyl-Leu-Leu-norleucinal (Ac-LLnL) in vitro.1 PublicationUniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteolysis involved in cellular protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-3893-MONOMER.
    UniPathwayiUPA00997.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit alphaUniRule annotation (EC:3.4.25.1UniRule annotation)
    Alternative name(s):
    20S proteasome alpha subunitUniRule annotation
    Proteasome core protein PrcAUniRule annotation
    Gene namesi
    Name:prcAUniRule annotation
    Ordered Locus Names:MSMEG_3894, MSMEI_3804
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. proteasome core complex, alpha-subunit complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Proteasome

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene are viable and grow at the wild-type rate, but show a large reduction in the rate of degradation of the pupylated substrates.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 246246Proteasome subunit alphaPRO_0000383483Add
    BLAST

    Interactioni

    Subunit structurei

    The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.UniRule annotation

    Protein-protein interaction databases

    STRINGi246196.MSMEG_3894.

    Structurei

    3D structure databases

    ProteinModelPortaliA0QZ46.
    SMRiA0QZ46. Positions 8-234.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1A family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000245319.
    KOiK03432.
    OMAiGRYNEFE.
    OrthoDBiEOG6NKQZG.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_00289_B. Proteasome_A_B.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR022296. Proteasome_asu_bac.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR03691. 20S_bact_alpha. 1 hit.
    PROSITEiPS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0QZ46-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFPYFISPE QAMRERSELA RKGIARGRSV VALAYSEGVL FVAENPSRSL    50
    QKVSELYDRV GFAAVGRFNE FDNLRRGGIQ FADTRGYAYD RRDVTGRQLA 100
    NVYAQTLGTI FTEQAKPYEV ELCVAEVAHY GETKAPELYR ITYDGSIADE 150
    PHFVVMGGTT EPIIAALNES YTENASLQDA VEIAVKALSA SAEGAEPRSL 200
    GPSTLEVAIL DAGRPRRAFR RITGAALEAL LPEQPQQADS GDKPTE 246
    Length:246
    Mass (Da):26,915
    Last modified:January 9, 2007 - v1
    Checksum:i7869013FEFD30231
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF009645 Genomic DNA. Translation: AAC45615.1.
    CP000480 Genomic DNA. Translation: ABK70795.1.
    CP001663 Genomic DNA. Translation: AFP40262.1.
    RefSeqiYP_006568557.1. NC_018289.1.
    YP_888184.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK70795; ABK70795; MSMEG_3894.
    AFP40262; AFP40262; MSMEI_3804.
    GeneIDi4535010.
    KEGGimsg:MSMEI_3804.
    msm:MSMEG_3894.
    PATRICi18080183. VBIMycSme59918_3834.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF009645 Genomic DNA. Translation: AAC45615.1 .
    CP000480 Genomic DNA. Translation: ABK70795.1 .
    CP001663 Genomic DNA. Translation: AFP40262.1 .
    RefSeqi YP_006568557.1. NC_018289.1.
    YP_888184.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0QZ46.
    SMRi A0QZ46. Positions 8-234.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_3894.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK70795 ; ABK70795 ; MSMEG_3894 .
    AFP40262 ; AFP40262 ; MSMEI_3804 .
    GeneIDi 4535010.
    KEGGi msg:MSMEI_3804.
    msm:MSMEG_3894.
    PATRICi 18080183. VBIMycSme59918_3834.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000245319.
    KOi K03432.
    OMAi GRYNEFE.
    OrthoDBi EOG6NKQZG.

    Enzyme and pathway databases

    UniPathwayi UPA00997 .
    BioCyci MSME246196:GJ4Y-3893-MONOMER.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_00289_B. Proteasome_A_B.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR022296. Proteasome_asu_bac.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR03691. 20S_bact_alpha. 1 hit.
    PROSITEi PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Inactivation of the 20S proteasome in Mycobacterium smegmatis."
      Knipfer N., Shrader T.E.
      Mol. Microbiol. 25:375-383(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, DISRUPTION PHENOTYPE.
    2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    5. "Proteasomal protein degradation in mycobacteria is dependent upon a prokaryotic ubiquitin-like protein."
      Burns K.E., Liu W.-T., Boshoff H.I.M., Dorrestein P.C., Barry C.E. III
      J. Biol. Chem. 284:3069-3075(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, PROTEIN SUBSTRATES, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPSA_MYCS2
    AccessioniPrimary (citable) accession number: A0QZ46
    Secondary accession number(s): I7G3V1, O30519
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3