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Protein

Acetylglutamate kinase

Gene

argB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ (argJ), Arginine biosynthesis bifunctional protein ArgJ (argJ)
  2. Acetylglutamate kinase (argB)
  3. N-acetyl-gamma-glutamyl-phosphate reductase (argC)
  4. Acetylornithine aminotransferase (argD)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei33 – 331Transition state stabilizerUniRule annotation
Binding sitei90 – 901SubstrateUniRule annotation
Binding sitei189 – 1891SubstrateUniRule annotation
Sitei250 – 2501Transition state stabilizerUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3773-MONOMER.
UniPathwayiUPA00068; UER00107.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylglutamate kinaseUniRule annotation (EC:2.7.2.8UniRule annotation)
Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferaseUniRule annotation
NAG kinaseUniRule annotation
Short name:
AGKUniRule annotation
Gene namesi
Name:argBUniRule annotation
Ordered Locus Names:MSMEG_3774, MSMEI_3685
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293Acetylglutamate kinasePRO_0000335647Add
BLAST

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_3774.

Structurei

3D structure databases

ProteinModelPortaliA0QYT0.
SMRiA0QYT0. Positions 11-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 692Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the acetylglutamate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CAS. Bacteria.
COG0548. LUCA.
HOGENOMiHOG000233259.
KOiK00930.
OMAiPKTECCI.
OrthoDBiPOG091H063W.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00082_B. ArgB_B. 1 hit.
InterProiIPR004662. AcgluKinase.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000728. NAGK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00761. argB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QYT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPSIDRGF KADVLASALP WLKQLHGKIV VVKYGGNAMT DDVLKAAFAA
60 70 80 90 100
DMVFLRNCGI HPVVVHGGGP QISAMLKRLG IEGDFKGGFR VTTPEVLDVA
110 120 130 140 150
RMVLFGQVGR ELVNLINAHG PYAVGVTGED AQLFTAVRRN VTVDGVATDI
160 170 180 190 200
GLVGDVEHVN AGSLLDLIAA GRIPVVSTIA PDADGVVHNI NADTAAAALA
210 220 230 240 250
EALGAEKLVM LTDVEGLYTD WPDRTSLVSE IDTGALTQLL PKLESGMVPK
260 270 280 290
IEACLRAVNG GVPSAHVIDG RVEHCVLVEL FTDEGTGTKV VAQ
Length:293
Mass (Da):30,658
Last modified:January 9, 2007 - v1
Checksum:i482D4F2946FF8D44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73548.1.
CP001663 Genomic DNA. Translation: AFP40144.1.
RefSeqiWP_003895218.1. NZ_CP009494.1.
YP_888068.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73548; ABK73548; MSMEG_3774.
AFP40144; AFP40144; MSMEI_3685.
GeneIDi4535272.
KEGGimsb:LJ00_18750.
msg:MSMEI_3685.
msm:MSMEG_3774.
PATRICi18079941. VBIMycSme59918_3713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73548.1.
CP001663 Genomic DNA. Translation: AFP40144.1.
RefSeqiWP_003895218.1. NZ_CP009494.1.
YP_888068.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QYT0.
SMRiA0QYT0. Positions 11-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73548; ABK73548; MSMEG_3774.
AFP40144; AFP40144; MSMEI_3685.
GeneIDi4535272.
KEGGimsb:LJ00_18750.
msg:MSMEI_3685.
msm:MSMEG_3774.
PATRICi18079941. VBIMycSme59918_3713.

Phylogenomic databases

eggNOGiENOG4105CAS. Bacteria.
COG0548. LUCA.
HOGENOMiHOG000233259.
KOiK00930.
OMAiPKTECCI.
OrthoDBiPOG091H063W.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00107.
BioCyciMSME246196:GJ4Y-3773-MONOMER.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00082_B. ArgB_B. 1 hit.
InterProiIPR004662. AcgluKinase.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000728. NAGK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00761. argB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARGB_MYCS2
AccessioniPrimary (citable) accession number: A0QYT0
Secondary accession number(s): I7GC40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.