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Protein

Acetylglutamate kinase

Gene

argB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.UniRule annotation

Catalytic activityi

ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ (argJ), Arginine biosynthesis bifunctional protein ArgJ (argJ)
  2. Acetylglutamate kinase (argB)
  3. N-acetyl-gamma-glutamyl-phosphate reductase (argC), N-acetyl-gamma-glutamyl-phosphate reductase (argC)
  4. Acetylornithine aminotransferase (argD)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Transition state stabilizerUniRule annotation1
Binding sitei90SubstrateUniRule annotation1
Binding sitei189SubstrateUniRule annotation1
Sitei250Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00107.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylglutamate kinaseUniRule annotation (EC:2.7.2.8UniRule annotation)
Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferaseUniRule annotation
NAG kinaseUniRule annotation
Short name:
NAGKUniRule annotation
Gene namesi
Name:argBUniRule annotation
Ordered Locus Names:MSMEG_3774, MSMEI_3685
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003356471 – 293Acetylglutamate kinaseAdd BLAST293

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_3774.

Structurei

3D structure databases

ProteinModelPortaliA0QYT0.
SMRiA0QYT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 69Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the acetylglutamate kinase family. ArgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CAS. Bacteria.
COG0548. LUCA.
HOGENOMiHOG000233259.
KOiK00930.
OMAiPKTECCI.
OrthoDBiPOG091H063W.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00082. ArgB. 1 hit.
InterProiView protein in InterPro
IPR036393. AceGlu_kinase-like_sf.
IPR004662. AcgluKinase.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PIRSFiPIRSF000728. NAGK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00761. argB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QYT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPSIDRGF KADVLASALP WLKQLHGKIV VVKYGGNAMT DDVLKAAFAA
60 70 80 90 100
DMVFLRNCGI HPVVVHGGGP QISAMLKRLG IEGDFKGGFR VTTPEVLDVA
110 120 130 140 150
RMVLFGQVGR ELVNLINAHG PYAVGVTGED AQLFTAVRRN VTVDGVATDI
160 170 180 190 200
GLVGDVEHVN AGSLLDLIAA GRIPVVSTIA PDADGVVHNI NADTAAAALA
210 220 230 240 250
EALGAEKLVM LTDVEGLYTD WPDRTSLVSE IDTGALTQLL PKLESGMVPK
260 270 280 290
IEACLRAVNG GVPSAHVIDG RVEHCVLVEL FTDEGTGTKV VAQ
Length:293
Mass (Da):30,658
Last modified:January 9, 2007 - v1
Checksum:i482D4F2946FF8D44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73548.1.
CP001663 Genomic DNA. Translation: AFP40144.1.
RefSeqiWP_003895218.1. NZ_CP009494.1.
YP_888068.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73548; ABK73548; MSMEG_3774.
AFP40144; AFP40144; MSMEI_3685.
GeneIDi4535272.
KEGGimsb:LJ00_18750.
msg:MSMEI_3685.
msm:MSMEG_3774.
PATRICifig|246196.19.peg.3713.

Similar proteinsi

Entry informationi

Entry nameiARGB_MYCS2
AccessioniPrimary (citable) accession number: A0QYT0
Secondary accession number(s): I7GC40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 9, 2007
Last modified: October 25, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families