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Protein

Acetylornithine aminotransferase

Gene

argD

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.UniRule annotation

Catalytic activityi

N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotationNote: Binds 1 pyridoxal phosphate per subunit.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ (argJ), Arginine biosynthesis bifunctional protein ArgJ (argJ)
  2. Acetylglutamate kinase (argB)
  3. N-acetyl-gamma-glutamyl-phosphate reductase (argC)
  4. Acetylornithine aminotransferase (argD)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei129Pyridoxal phosphate; via carbonyl oxygenUniRule annotation1
Binding sitei132N2-acetyl-L-ornithineUniRule annotation1
Binding sitei271N2-acetyl-L-ornithineUniRule annotation1
Binding sitei272Pyridoxal phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00068; UER00109.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylornithine aminotransferaseUniRule annotation (EC:2.6.1.11UniRule annotation)
Short name:
ACOATUniRule annotation
Gene namesi
Name:argDUniRule annotation
Ordered Locus Names:MSMEG_3773, MSMEI_3684
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003968161 – 390Acetylornithine aminotransferaseAdd BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei243N6-(pyridoxal phosphate)lysineUniRule annotation1
Cross-linki304Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_3773.

Structurei

3D structure databases

ProteinModelPortaliA0QYS9.
SMRiA0QYS9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 104Pyridoxal phosphate bindingUniRule annotation2
Regioni214 – 217Pyridoxal phosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8Y. Bacteria.
COG4992. LUCA.
HOGENOMiHOG000020206.
KOiK00821.
OMAiLRHVECI.
OrthoDBiPOG091H03ZS.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_01107. ArgD_aminotrans_3. 1 hit.
InterProiView protein in InterPro
IPR004636. AcOrn/SuccOrn_fam.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_sub2.
PfamiView protein in Pfam
PF00202. Aminotran_3. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00707. argD. 1 hit.
PROSITEiView protein in PROSITE
PS00600. AA_TRANSFER_CLASS_3. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QYS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLQSRWEAV MMNNYGTPPL SLVSGEGAVV TDADGREYLD LLGGIAVNLL
60 70 80 90 100
GHRHPAVIEA VTTQLDTLGH TSNLYATEPG IALAEALVGQ LGTQARVFFC
110 120 130 140 150
NSGTEANEVA FKITRLTGKT KIVAAEGAFH GRTMGSLALT GQPSKQAPFE
160 170 180 190 200
PLPGNVMHVP YGDVAALEAA VDDQTAAVFL EPIMGEGGVV VPPAGYLVAA
210 220 230 240 250
REITSKHGAL LVLDEVQTGV GRTGAFFAHQ HDGIVPDVVT MAKGLGGGLP
260 270 280 290 300
IGACLAVGAT GDLLTPGLHG STFGGNPVCT AAGLAVLKTL AAEDLVARAG
310 320 330 340 350
VLGKTLSHGI EELGHPLVDK VRGKGLLQGI VLTVPSAKAV ETAARDAGFL
360 370 380 390
VNAAAPEVVR LAPPLIITEG QIEAFITALP AVLDTAAEDS
Length:390
Mass (Da):39,796
Last modified:January 9, 2007 - v1
Checksum:i51D702618A820F13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75110.1.
CP001663 Genomic DNA. Translation: AFP40143.1.
RefSeqiWP_011729315.1. NZ_CP009494.1.
YP_888067.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK75110; ABK75110; MSMEG_3773.
AFP40143; AFP40143; MSMEI_3684.
GeneIDi4531216.
KEGGimsb:LJ00_18745.
msg:MSMEI_3684.
msm:MSMEG_3773.
PATRICi18079939. VBIMycSme59918_3712.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75110.1.
CP001663 Genomic DNA. Translation: AFP40143.1.
RefSeqiWP_011729315.1. NZ_CP009494.1.
YP_888067.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QYS9.
SMRiA0QYS9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3773.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK75110; ABK75110; MSMEG_3773.
AFP40143; AFP40143; MSMEI_3684.
GeneIDi4531216.
KEGGimsb:LJ00_18745.
msg:MSMEI_3684.
msm:MSMEG_3773.
PATRICi18079939. VBIMycSme59918_3712.

Phylogenomic databases

eggNOGiENOG4105C8Y. Bacteria.
COG4992. LUCA.
HOGENOMiHOG000020206.
KOiK00821.
OMAiLRHVECI.
OrthoDBiPOG091H03ZS.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00109.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_01107. ArgD_aminotrans_3. 1 hit.
InterProiView protein in InterPro
IPR004636. AcOrn/SuccOrn_fam.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_sub2.
PfamiView protein in Pfam
PF00202. Aminotran_3. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00707. argD. 1 hit.
PROSITEiView protein in PROSITE
PS00600. AA_TRANSFER_CLASS_3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARGD_MYCS2
AccessioniPrimary (citable) accession number: A0QYS9
Secondary accession number(s): I7FN58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: April 12, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.