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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.1 Publication

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Enzyme regulationi

Inhibited by arginine, norvaline.1 Publication

Kineticsi

  1. KM=0.20 mM for carbamoyl phosphate1 Publication
  2. KM=0.25 mM for L-ornithine1 Publication

    Pathwayi: L-arginine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Ornithine carbamoyltransferase (argF)
    2. Argininosuccinate synthase (argG)
    3. Argininosuccinate lyase (argH)
    This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei6Carbamoyl phosphateBy similarity1
    Binding sitei66Carbamoyl phosphateBy similarity1
    Binding sitei77Carbamoyl phosphateBy similarity1
    Binding sitei101Carbamoyl phosphateBy similarity1
    Binding sitei160OrnithineBy similarity1
    Binding sitei224OrnithineBy similarity1
    Binding sitei274Carbamoyl phosphateBy similarity1
    Binding sitei292SubstrateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00068; UER00112.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ornithine carbamoyltransferase (EC:2.1.3.3)
    Short name:
    OTCase
    Gene namesi
    Name:argF
    Ordered Locus Names:MSMEG_3772, MSMEI_3683
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
    Proteomesi
    • UP000006158 Componenti: Chromosome
    • UP000000757 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_10000730301 – 307Ornithine carbamoyltransferaseAdd BLAST307

    Interactioni

    Protein-protein interaction databases

    STRINGi246196.MSMEG_3772.

    Structurei

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei27Important for structural integrityBy similarity1
    Sitei141Important for structural integrityBy similarity1

    3D structure databases

    ProteinModelPortaliA0QYS8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni34 – 37Carbamoyl phosphate bindingBy similarity4
    Regioni50 – 54Carbamoyl phosphate bindingBy similarity5
    Regioni128 – 131Carbamoyl phosphate bindingBy similarity4
    Regioni228 – 229Ornithine bindingBy similarity2
    Regioni248 – 249Carbamoyl phosphate bindingBy similarity2
    Regioni263 – 266Carbamoyl phosphate bindingBy similarity4

    Sequence similaritiesi

    Belongs to the ATCase/OTCase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105DBV. Bacteria.
    COG0078. LUCA.
    HOGENOMiHOG000022686.
    KOiK00611.
    OMAiEHPCQIL.
    OrthoDBiPOG091H0543.

    Family and domain databases

    Gene3Di3.40.50.1370. 2 hits.
    HAMAPiMF_01109. OTCase. 1 hit.
    InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024904. OTCase_ArgI.
    [Graphical view]
    PfamiPF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view]
    PRINTSiPR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMiSSF53671. SSF53671. 1 hit.
    TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
    PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0QYS8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIRHFLRDDD LSPEEQAEVL TLAADLKKTP FSRRPLEGPR GVAVIFEKNS
    60 70 80 90 100
    TRTRFSFEMG IAQLGGHAIV VDGRSTQLGR EETLEDTGAV LSRYVDAIVW
    110 120 130 140 150
    RTFAQERLTA MASGASVPIV NALSDEFHPC QVLADLQTLA ERKGKLAGLR
    160 170 180 190 200
    MTYFGDGANN MAHSLMLGGV TAGVHVTIAA PDGFEPDPRF VDAARRRAAE
    210 220 230 240 250
    TGATVALTKD AKAGADGADV LVTDTWTSMG QENDGLDRVR PFRPFQVNAD
    260 270 280 290 300
    LLELADPAAV VLHCLPAHRG HEITDEVIDG PQSAVFDEAE NRLHAQKALL

    VWLLEKR
    Length:307
    Mass (Da):33,388
    Last modified:January 9, 2007 - v1
    Checksum:i355FD07788911954
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA. Translation: ABK75220.1.
    CP001663 Genomic DNA. Translation: AFP40142.1.
    RefSeqiWP_011729314.1. NZ_CP009494.1.
    YP_888066.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK75220; ABK75220; MSMEG_3772.
    AFP40142; AFP40142; MSMEI_3683.
    GeneIDi4533190.
    KEGGimsb:LJ00_18740.
    msg:MSMEI_3683.
    msm:MSMEG_3772.
    PATRICi18079937. VBIMycSme59918_3711.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA. Translation: ABK75220.1.
    CP001663 Genomic DNA. Translation: AFP40142.1.
    RefSeqiWP_011729314.1. NZ_CP009494.1.
    YP_888066.1. NC_008596.1.

    3D structure databases

    ProteinModelPortaliA0QYS8.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi246196.MSMEG_3772.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiABK75220; ABK75220; MSMEG_3772.
    AFP40142; AFP40142; MSMEI_3683.
    GeneIDi4533190.
    KEGGimsb:LJ00_18740.
    msg:MSMEI_3683.
    msm:MSMEG_3772.
    PATRICi18079937. VBIMycSme59918_3711.

    Phylogenomic databases

    eggNOGiENOG4105DBV. Bacteria.
    COG0078. LUCA.
    HOGENOMiHOG000022686.
    KOiK00611.
    OMAiEHPCQIL.
    OrthoDBiPOG091H0543.

    Enzyme and pathway databases

    UniPathwayiUPA00068; UER00112.

    Family and domain databases

    Gene3Di3.40.50.1370. 2 hits.
    HAMAPiMF_01109. OTCase. 1 hit.
    InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
    IPR006130. Asp/Orn_carbamoylTrfase.
    IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
    IPR002292. Orn/put_carbamltrans.
    IPR024904. OTCase_ArgI.
    [Graphical view]
    PfamiPF00185. OTCace. 1 hit.
    PF02729. OTCace_N. 1 hit.
    [Graphical view]
    PRINTSiPR00100. AOTCASE.
    PR00102. OTCASE.
    SUPFAMiSSF53671. SSF53671. 1 hit.
    TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
    PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiOTC_MYCS2
    AccessioniPrimary (citable) accession number: A0QYS8
    Secondary accession number(s): I7G3K5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: January 9, 2007
    Last modified: November 2, 2016
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.