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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.1 Publication

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.1 Publication

Enzyme regulationi

Inhibited by arginine, norvaline.1 Publication

Kineticsi

  1. KM=0.20 mM for carbamoyl phosphate1 Publication
  2. KM=0.25 mM for L-ornithine1 Publication

    Pathwayi: L-arginine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Ornithine carbamoyltransferase (argF)
    2. Argininosuccinate synthase (argG)
    3. Argininosuccinate lyase (argH)
    This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei77Carbamoyl phosphateUniRule annotation1
    Binding sitei101Carbamoyl phosphateUniRule annotation1
    Binding sitei160OrnithineUniRule annotation1
    Binding sitei224OrnithineUniRule annotation1
    Binding sitei292Carbamoyl phosphateUniRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase
    Biological processAmino-acid biosynthesis, Arginine biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00068; UER00112

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ornithine carbamoyltransferase1 Publication (EC:2.1.3.31 Publication)
    Short name:
    OTCase1 Publication
    Gene namesi
    Name:argF1 Publication
    Ordered Locus Names:MSMEG_3772, MSMEI_3683
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
    Proteomesi
    • UP000006158 Componenti: Chromosome
    • UP000000757 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_10000730301 – 307Ornithine carbamoyltransferaseAdd BLAST307

    Interactioni

    Protein-protein interaction databases

    STRINGi246196.MSMEG_3772

    Structurei

    3D structure databases

    ProteinModelPortaliA0QYS8
    SMRiA0QYS8
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni50 – 53Carbamoyl phosphate bindingUniRule annotation4
    Regioni128 – 131Carbamoyl phosphate bindingUniRule annotation4
    Regioni228 – 229Ornithine bindingUniRule annotation2
    Regioni264 – 265Carbamoyl phosphate bindingUniRule annotation2

    Sequence similaritiesi

    Belongs to the ATCase/OTCase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105DBV Bacteria
    COG0078 LUCA
    HOGENOMiHOG000022686
    KOiK00611
    OMAiDGNNVCN
    OrthoDBiPOG091H0543

    Family and domain databases

    Gene3Di3.40.50.1370, 3 hits
    HAMAPiMF_01109 OTCase, 1 hit
    InterProiView protein in InterPro
    IPR006132 Asp/Orn_carbamoyltranf_P-bd
    IPR006130 Asp/Orn_carbamoylTrfase
    IPR036901 Asp/Orn_carbamoylTrfase_sf
    IPR006131 Asp_carbamoyltransf_Asp/Orn-bd
    IPR002292 Orn/put_carbamltrans
    IPR024904 OTCase_ArgI
    PfamiView protein in Pfam
    PF00185 OTCace, 1 hit
    PF02729 OTCace_N, 1 hit
    PRINTSiPR00100 AOTCASE
    PR00102 OTCASE
    SUPFAMiSSF53671 SSF53671, 1 hit
    TIGRFAMsiTIGR00658 orni_carb_tr, 1 hit
    PROSITEiView protein in PROSITE
    PS00097 CARBAMOYLTRANSFERASE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    A0QYS8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIRHFLRDDD LSPEEQAEVL TLAADLKKTP FSRRPLEGPR GVAVIFEKNS
    60 70 80 90 100
    TRTRFSFEMG IAQLGGHAIV VDGRSTQLGR EETLEDTGAV LSRYVDAIVW
    110 120 130 140 150
    RTFAQERLTA MASGASVPIV NALSDEFHPC QVLADLQTLA ERKGKLAGLR
    160 170 180 190 200
    MTYFGDGANN MAHSLMLGGV TAGVHVTIAA PDGFEPDPRF VDAARRRAAE
    210 220 230 240 250
    TGATVALTKD AKAGADGADV LVTDTWTSMG QENDGLDRVR PFRPFQVNAD
    260 270 280 290 300
    LLELADPAAV VLHCLPAHRG HEITDEVIDG PQSAVFDEAE NRLHAQKALL

    VWLLEKR
    Length:307
    Mass (Da):33,388
    Last modified:January 9, 2007 - v1
    Checksum:i355FD07788911954
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP000480 Genomic DNA Translation: ABK75220.1
    CP001663 Genomic DNA Translation: AFP40142.1
    RefSeqiWP_011729314.1, NZ_CP009494.1
    YP_888066.1, NC_008596.1

    Genome annotation databases

    EnsemblBacteriaiABK75220; ABK75220; MSMEG_3772
    AFP40142; AFP40142; MSMEI_3683
    GeneIDi4533190
    KEGGimsb:LJ00_18740
    msg:MSMEI_3683
    msm:MSMEG_3772
    PATRICifig|246196.19.peg.3711

    Similar proteinsi

    Entry informationi

    Entry nameiOTC_MYCS2
    AccessioniPrimary (citable) accession number: A0QYS8
    Secondary accession number(s): I7G3K5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: January 9, 2007
    Last modified: May 23, 2018
    This is version 80 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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