A0QYS5 (ARLY_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate lyase
Short name=ASAL
EC=4.3.2.1

Alternative name(s):
Arginosuccinase

Gene names

Name:	argH
Ordered Locus Names:	MSMEG_3769

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. HAMAP MF_00006
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3. HAMAP MF_00006
Subcellular location	Cytoplasm By similarity HAMAP MF_00006.
Sequence similarities	Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process via ornithine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	argininosuccinate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Argininosuccinate lyase HAMAP MF_00006		PRO_1000000506

Sequences

	Sequence		Length	Mass (Da)
	A0QYS5 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: D151C60E839A0C79	FASTA	469	49,744
	10 20 30 40 50 60 MSTNEGSLWG GRFADGPSDA LAALSKSTHF DWALAPYDIK ASKAHARVLH RAGLLTDEQR 70 80 90 100 110 120 DGLLAGLDSL GSDVADGSFE PLPTDEDVHG ALERGLIDRV GPDLGGRLRA GRSRNDQVAT 130 140 150 160 170 180 LFRMWLRDAV RRVADGCLEV VNALAVQAAA HPTAIMPGKT HLQAAQPILL AHHLLAHAHP 190 200 210 220 230 240 LLRDVDRLAD FDDRTAVSPY GSGALAGSSL GLDPDAIAED LGFASAADNS VDATASRDFA 250 260 270 280 290 300 AEAAFVFAQI GVDLSRLAED IILWSSTEFG YVTLHDAWST GSSIMPQKKN PDIAELARGK 310 320 330 340 350 360 SGRLIGNLTG LLATLKAQPL AYNRDLQEDK EPVFDSVAQL ELLLPAMAGL VGTLTFDEER 370 380 390 400 410 420 MAELAPAGYT LATDIAEWLV RQGVPFRIAH EAAGAAVKVA EGRGVGLDAL TDDEFASINP 430 440 450 460 ALTPDVREVL TVEGSVNARN ARGGTAPTQV AKQLGVVRKA MEELRIRLS

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed: 18955433] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK73307.1.
RefSeq	YP_888063.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QYS5.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QYS5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000042732; EBMYCP00000041069; EBMYCG00000042727.
GeneID	4533483.
GenomeReviews	Gene locus MSMEG_3769 in contig CP000480_GR.
KEGG	msm:MSMEG_3769.
NMPDR	fig|246196.1.peg.3775.
PATRIC	18079931. VBIMycSme59918_3708.
TIGR	MSMEG_3769.
Phylogenomic databases
eggNOG	COG0165.
GeneTree	EBGT00050000016253.
HOGENOM	HBG539632.
OMA	MPGRTHL.
ProtClustDB	PRK00855.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_3769-MONOMER.
Family and domain databases
HAMAP	MF_00006. Arg_succ_lyase. [Tree]
InterPro	IPR009049. Argininosuccinate_lyase. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR022761. Lyase1_N. [Graphical view]
KO	K01755.
PANTHER	PTHR11444:SF3. argH. 1 hit.
Pfam	PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00838. ArgH. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLY_MYCS2

Accession

Primary (citable) accession number: A0QYS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program