Reviewed, UniProtKB/Swiss-Prot A0QYP1 (KATG3_MYCS2)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 3
      Short name=CP 3
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 3

Gene names

Name:	katG3
Ordered Locus Names:	MSMEG_3729

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Complete proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 744	744	Catalase-peroxidase 3 HAMAP MF_01961		PRO_0000354840
Sites
	Active site	107	1	Proton acceptor By similarity
	Metal binding	269	1	Iron (heme axial ligand) By similarity
	Site	103	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	106 ↔ 228		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-254) By similarity
	Cross-link	228 ↔ 254		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-106) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QYP1-1 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: A980D38FD8FCBF8E	FASTA	744	82,295
	10 20 30 40 50 60 MPESPDAYVN RTYDQTVAVR RSLKRRNSPA ASEDGLGWPR RLNLRILAQP CRTSSPLGED 70 80 90 100 110 120 FDYAKEFLSL DLDELARDID EVLTTSQDWW PADFGHYGPL VLRMAWHFAG TYRIGDGRGG 130 140 150 160 170 180 AGAGMLRFAP LNSFPDNRNL DKARRLLWPV KKKYGRKISW SDLMIFAGNR ALESMGCRTF 190 200 210 220 230 240 GFAGGREDAW EADETYWGPE STWLADERHS GVRDLDQPLA ASEMGLIYVD PQGPATLPDP 250 260 270 280 290 300 LASARDIRET FRRMGMNDEE TVALIAGGHT FGKSHGPTDP SRCLGPEPEG APLEALGLGW 310 320 330 340 350 360 VNSFGTGNGA DTVTSGLDGI WTATPTKWDM SFLTTLFAYE WDVALSPAGM WQWVPRNGAG 370 380 390 400 410 420 AGTVPDPYDP SRTHAPTMLT TDLALQEDPR YRVIALRFLE NPDEFADTFA RAWFKLTHID 430 440 450 460 470 480 MGPIQRYLGP LVPTERMIWQ DPVPHVDHEL ADADDVAALK REILGSGLSV SQLVTTAWAS 490 500 510 520 530 540 ASTFRNSDKR GGANGARIRL EPQRSWAVNE PEKLAIVLDR LERIRRRFND SHRGGKQISA 550 560 570 580 590 600 ADLIMLGGCA AVEHAAAEAG HPIEVPCRLG RTDAPQEWTD IEWFSALEPT ADAFRNYVGE 610 620 630 640 650 660 GNRPPPEHLL VDRASQLTLT APQMTVLLGG LRVLGANHGG SPLGVFTASP GALSNDFFVN 670 680 690 700 710 720 LLDVNIEWTP RADTADWTAA YEGRDRRTGE VTWIASRVDL SFASDPVLRA ISEVYASADA 730 740 EEKFVRDFVS AWDKVMNLDL FDRT

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000480 Genomic DNA. Translation: ABK74103.1. Different initiation.
RefSeq	YP_888029.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0QYP1.
Genome annotation databases
GeneID	4536221.
GenomeReviews	Gene locus MSMEG_3729 in contig CP000480_GR.
KEGG	msm:MSMEG_3729.
NMPDR	fig|246196.1.peg.3735.
TIGR	MSMEG_3729.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG3_MYCS2

Accession

Primary (citable) accession number: A0QYP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	October 13, 2009
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)