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Protein

Glycogen accumulation regulator GarA

Gene

garA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of glutamate metabolism. Acts as a phosphorylation-dependent molecular switch that modulates the activities of Kgd and Gdh.1 Publication

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3647-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen accumulation regulator GarA
Gene namesi
Name:garA
Ordered Locus Names:MSMEG_3647, MSMEI_3561
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 158158Glycogen accumulation regulator GarAPRO_0000419534Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201Phosphothreonine; by PknG1 Publication
Modified residuei21 – 211Phosphothreonine; by PknBBy similarity

Post-translational modificationi

Phosphorylated on Thr-21 by PknB (By similarity). Phosphorylated on Thr-20 by PknG. Phosphorylation at either Thr-20 or Thr-21 prevents binding to target enzymes.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiA0QYG2.

Interactioni

Subunit structurei

Monomer (By similarity). Binds via its FHA domain to Kgd, Gdh, and the N-terminal region of PknG.By similarity

Protein-protein interaction databases

IntActiA0QYG2. 3 interactions.
STRINGi246196.MSMEG_3647.

Structurei

3D structure databases

ProteinModelPortaliA0QYG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini76 – 12550FHAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108UW8. Bacteria.
COG1716. LUCA.
HOGENOMiHOG000242873.
OrthoDBiEOG6SR96T.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QYG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKDSNLGA DQSEDVTVET TSVFRADFLN ELDAPAAAGT EGAVSGVEGL
60 70 80 90 100
PSGSALLVVK RGPNAGSRFL LDQPTTSAGR HPDSDIFLDD VTVSRRHAEF
110 120 130 140 150
RLEGGEFQVV DVGSLNGTYV NREPVDSAVL ANGDEVQIGK FRLVFLTGPK

SDDSGSNA
Length:158
Mass (Da):16,584
Last modified:October 3, 2012 - v2
Checksum:i884B68B7C37084BE
GO

Sequence cautioni

The sequence ABK73997.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73997.1. Different initiation.
CP001663 Genomic DNA. Translation: AFP40024.1.
RefSeqiWP_011729230.1. NC_008596.1.
WP_014877774.1. NZ_CP009494.1.
YP_887950.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73997; ABK73997; MSMEG_3647.
AFP40024; AFP40024; MSMEI_3561.
GeneIDi4536307.
KEGGimsb:LJ00_18135.
msg:MSMEI_3561.
msm:MSMEG_3647.
PATRICi18079697. VBIMycSme59918_3595.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73997.1. Different initiation.
CP001663 Genomic DNA. Translation: AFP40024.1.
RefSeqiWP_011729230.1. NC_008596.1.
WP_014877774.1. NZ_CP009494.1.
YP_887950.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QYG2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiA0QYG2. 3 interactions.
STRINGi246196.MSMEG_3647.

PTM databases

iPTMnetiA0QYG2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73997; ABK73997; MSMEG_3647.
AFP40024; AFP40024; MSMEI_3561.
GeneIDi4536307.
KEGGimsb:LJ00_18135.
msg:MSMEI_3561.
msm:MSMEG_3647.
PATRICi18079697. VBIMycSme59918_3595.

Phylogenomic databases

eggNOGiENOG4108UW8. Bacteria.
COG1716. LUCA.
HOGENOMiHOG000242873.
OrthoDBiEOG6SR96T.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3647-MONOMER.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. Cited for: FUNCTION, INTERACTION WITH KGD; GDH AND PKNG, PHOSPHORYLATION AT THR-20.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiGARA_MYCS2
AccessioniPrimary (citable) accession number: A0QYG2
Secondary accession number(s): I7GBT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: October 3, 2012
Last modified: June 8, 2016
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.