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Protein

Catalase-peroxidase 2

Gene

katG2

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria.

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bNote: Binds 2 heme B (iron-protoporphyrin IX) groups per tetramer.

Kineticsi

  1. KM=1.4 mM for H2O2 for the catalase reaction1 Publication
  2. KM=0.19 mM for o-dianisidine for the peroxidase reaction1 Publication
  3. KM=0.17 mM for NADPH for the peroxidase reaction1 Publication
  4. KM=0.23 mM for NADH for the peroxidase reaction1 Publication
  5. KM=0.31 mM for pyrogallol for the peroxidase reaction1 Publication
  6. KM=0.11 mM for 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) for the peroxidase reaction1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei110Transition state stabilizerUniRule annotation1
    Active sitei114Proton acceptorUniRule annotation1
    Metal bindingi279Iron (heme axial ligand)UniRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase, Peroxidase
    Biological processHydrogen peroxide
    LigandHeme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMSME246196:G1H7P-3463-MONOMER

    Protein family/group databases

    PeroxiBasei3570 MsmCP02_mc2155

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Catalase-peroxidase 2UniRule annotation (EC:1.11.1.21UniRule annotation)
    Short name:
    CP 2UniRule annotation
    Alternative name(s):
    Peroxidase/catalase 2UniRule annotation
    Gene namesi
    Name:katG2UniRule annotation
    Synonyms:katH
    Ordered Locus Names:MSMEG_3461, MSMEI_3380
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
    Proteomesi
    • UP000006158 Componenti: Chromosome
    • UP000000757 Componenti: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Defects cause isoniazid (INH) resistance.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved2 Publications
    ChainiPRO_00003450942 – 748Catalase-peroxidase 2Add BLAST747

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Cross-linki113 ↔ 238Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-264)UniRule annotation
    Cross-linki238 ↔ 264Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-113)UniRule annotation

    Post-translational modificationi

    Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

    Proteomic databases

    PRIDEiA0QXX7

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi246196.MSMEG_3461

    Structurei

    3D structure databases

    ProteinModelPortaliA0QXX7
    SMRiA0QXX7
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105C1X Bacteria
    COG0376 LUCA
    HOGENOMiHOG000218110
    KOiK03782
    OMAiEPEEIFW
    OrthoDBiPOG091H05R1

    Family and domain databases

    HAMAPiMF_01961 Catal_peroxid, 1 hit
    InterProiView protein in InterPro
    IPR000763 Catalase_peroxidase
    IPR010255 Haem_peroxidase
    IPR002016 Haem_peroxidase_pln/fun/bac
    IPR019794 Peroxidases_AS
    IPR019793 Peroxidases_heam-ligand_BS
    PANTHERiPTHR30555 PTHR30555, 1 hit
    PfamiView protein in Pfam
    PF00141 peroxidase, 2 hits
    PRINTSiPR00460 BPEROXIDASE
    PR00458 PEROXIDASE
    SUPFAMiSSF48113 SSF48113, 2 hits
    TIGRFAMsiTIGR00198 cat_per_HPI, 1 hit
    PROSITEiView protein in PROSITE
    PS00435 PEROXIDASE_1, 1 hit
    PS00436 PEROXIDASE_2, 1 hit
    PS50873 PEROXIDASE_4, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A0QXX7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSDTSDSRP PNPDTKTAST SESENPAIPS PKPKSGAPLR NQDWWPNQID
    60 70 80 90 100
    VSRLHPHPPQ GNPLGEDFDY AEEFAKLDVN ALKADLTALM TQSQDWWPAD
    110 120 130 140 150
    YGHYGGLFIR MSWHSAGTYR IHDGRGGGGQ GAQRFAPINS WPDNVSLDKA
    160 170 180 190 200
    RRLLWPIKQK YGNKISWADL LVFTGNVALE SMGFKTFGFG FGREDIWEPE
    210 220 230 240 250
    EILFGEEDEW LGTDKRYGGG EQRQLAEPYG ATTMGLIYVN PEGPEGQPDP
    260 270 280 290 300
    LAAAHDIRET FGRMAMNDEE TAALIVGGHT FGKTHGAGDA SLVGPEPEAA
    310 320 330 340 350
    PIEQQGLGWK SSYGTGKGPD TITSGLEVVW TNTPTKWDNS FLEILYGYEW
    360 370 380 390 400
    ELTKSPAGAW QFTAKDGAGA GTIPDPFGGP GRNPTMLVTD ISMRVDPIYG
    410 420 430 440 450
    KITRRWLDHP EELSEAFAKA WYKLLHRDMG PISRYLGPWV AEPQLWQDPV
    460 470 480 490 500
    PAVDHPLVDD QDIAALKSTV LDSGLSTGQL IKTAWASAAS YRNTDKRGGA
    510 520 530 540 550
    NGARVRLEPQ KNWDVNEPAE LATVLPVLER IQQDFNASAS GGKKVSLADL
    560 570 580 590 600
    IVLAGSAAIE KAAKDGGYNV TVPFAPGRTD ASQENTDVES FAVLEPRADG
    610 620 630 640 650
    FRNYVRPGEK VQLEKMLLER AYFLGVTAPQ LTALVGGLRA LDVNHGGTKH
    660 670 680 690 700
    GVFTDRPGAL TNDFFVNLLD MGTEWKTSET TENVYEGVDR KTGQLKWTAT
    710 720 730 740
    ANDLVFGSHS VLRAVAEVYA QSDNGERFVN DFVKAWVKVM NNDRFDLK
    Length:748
    Mass (Da):81,998
    Last modified:January 9, 2007 - v1
    Checksum:i73E44205C96B2FD3
    GO

    Mass spectrometryi

    Molecular mass is 81889 Da from positions 2 - 748. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ311851 Genomic DNA Translation: CAC44462.1
    CP000480 Genomic DNA Translation: ABK73887.1
    CP001663 Genomic DNA Translation: AFP39843.1
    RefSeqiWP_011729086.1, NZ_CP009494.1
    YP_887765.1, NC_008596.1

    Genome annotation databases

    EnsemblBacteriaiABK73887; ABK73887; MSMEG_3461
    AFP39843; AFP39843; MSMEI_3380
    GeneIDi4536428
    KEGGimsb:LJ00_17220
    msg:MSMEI_3380
    msm:MSMEG_3461
    PATRICifig|246196.19.peg.3418

    Similar proteinsi

    Entry informationi

    Entry nameiKATG2_MYCS2
    AccessioniPrimary (citable) accession number: A0QXX7
    Secondary accession number(s): I7FMA5, Q93JZ3, Q9R4J9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: January 9, 2007
    Last modified: May 23, 2018
    This is version 78 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

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