Reviewed, UniProtKB/Swiss-Prot A0QXX7 (KATG2_MYCS2)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Synonyms:	katH
Ordered Locus Names:	MSMEG_3461

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Complete proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. May play a role in the intracellular survival of mycobacteria. HAMAP MF_01961
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 2 heme B (iron-protoporphyrin IX) groups per tetramer. Ref.3
Subunit structure	Homotetramer. Ref.3
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Disruption phenotype	Defects cause isoniazid (INH) resistance. Ref.3
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.
Biophysicochemical properties	Kinetic parameters: KM=1.4 mM for H2O2 for the catalase reaction HAMAP MF_01961 KM=0.19 mM for o-dianisidine for the peroxidase reaction KM=0.17 mM for NADPH for the peroxidase reaction KM=0.23 mM for NADH for the peroxidase reaction KM=0.31 mM for pyrogallol for the peroxidase reaction KM=0.11 mM for 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) for the peroxidase reaction
Mass spectrometry	Molecular mass is 81889 Da from positions 2 - 748. Determined by ESI. Ref.3

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3 Ref.4
	Chain	2 – 748	747	Catalase-peroxidase 2 HAMAP MF_01961		PRO_0000345094
Sites
	Active site	114	1	Proton acceptor By similarity
	Metal binding	279	1	Iron (heme axial ligand) By similarity
	Site	110	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	113 ↔ 238		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-264) By similarity
	Cross-link	238 ↔ 264		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-113) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QXX7-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 73E44205C96B2FD3	FASTA	748	81,998
	10 20 30 40 50 60 MSSDTSDSRP PNPDTKTAST SESENPAIPS PKPKSGAPLR NQDWWPNQID VSRLHPHPPQ 70 80 90 100 110 120 GNPLGEDFDY AEEFAKLDVN ALKADLTALM TQSQDWWPAD YGHYGGLFIR MSWHSAGTYR 130 140 150 160 170 180 IHDGRGGGGQ GAQRFAPINS WPDNVSLDKA RRLLWPIKQK YGNKISWADL LVFTGNVALE 190 200 210 220 230 240 SMGFKTFGFG FGREDIWEPE EILFGEEDEW LGTDKRYGGG EQRQLAEPYG ATTMGLIYVN 250 260 270 280 290 300 PEGPEGQPDP LAAAHDIRET FGRMAMNDEE TAALIVGGHT FGKTHGAGDA SLVGPEPEAA 310 320 330 340 350 360 PIEQQGLGWK SSYGTGKGPD TITSGLEVVW TNTPTKWDNS FLEILYGYEW ELTKSPAGAW 370 380 390 400 410 420 QFTAKDGAGA GTIPDPFGGP GRNPTMLVTD ISMRVDPIYG KITRRWLDHP EELSEAFAKA 430 440 450 460 470 480 WYKLLHRDMG PISRYLGPWV AEPQLWQDPV PAVDHPLVDD QDIAALKSTV LDSGLSTGQL 490 500 510 520 530 540 IKTAWASAAS YRNTDKRGGA NGARVRLEPQ KNWDVNEPAE LATVLPVLER IQQDFNASAS 550 560 570 580 590 600 GGKKVSLADL IVLAGSAAIE KAAKDGGYNV TVPFAPGRTD ASQENTDVES FAVLEPRADG 610 620 630 640 650 660 FRNYVRPGEK VQLEKMLLER AYFLGVTAPQ LTALVGGLRA LDVNHGGTKH GVFTDRPGAL 670 680 690 700 710 720 TNDFFVNLLD MGTEWKTSET TENVYEGVDR KTGQLKWTAT ANDLVFGSHS VLRAVAEVYA 730 740 QSDNGERFVN DFVKAWVKVM NNDRFDLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The catalase-peroxidase of Mycobacterium smegmatis." Honore N. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Purification and characterization of the Mycobacterium smegmatis catalase-peroxidase involved in isoniazid activation." Marcinkeviciene J.A., Magliozzo R.S., Blanchard J.S. J. Biol. Chem. 270:22290-22295(1995) [PubMed: 7673210] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20, BIOPHYSICOCHEMICAL PROPERTIES, HEME-BINDING, SUBUNIT, MASS SPECTROMETRY, DISRUPTION PHENOTYPE.
[4]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed: 18955433] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS] BY MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ311851 Genomic DNA. Translation: CAC44462.1. CP000480 Genomic DNA. Translation: ABK73887.1.
RefSeq	YP_887765.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0QXX7.
Genome annotation databases
GeneID	4536428.
GenomeReviews	Gene locus MSMEG_3461 in contig CP000480_GR.
KEGG	msm:MSMEG_3461.
NMPDR	fig|246196.1.peg.3467.
TIGR	MSMEG_3461.
Phylogenomic databases
OMA	FEWELTK.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_MYCS2

Accession

Primary (citable) accession number: A0QXX7
Secondary accession number(s): Q93JZ3, Q9R4J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	January 9, 2007
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)