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A0QX70 (BIOB_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:MSMEG_3194, MSMEI_3112
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence AFP39576.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Biotin synthase HAMAP-Rule MF_01694
PRO_0000381482

Sites

Metal binding891Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding931Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding961Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1321Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1651Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2241Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2941Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A0QX70 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: DC0B6115C06BEB0D

FASTA35338,396
        10         20         30         40         50         60 
MFEVFRDLFN PQEDGVTQAA INGTDVLDVA REQVLERGVG LDQAQTLQVL QLPDERLDDL 

        70         80         90        100        110        120 
LSLAHEVRMR WCGPDVEVEG IISLKTGGCP EDCHFCSQSG LFASPVRSAW LDVPSLVEAA 

       130        140        150        160        170        180 
KQTAKTGATE FCIVAAVRGP DERLLAQVAA GIEAIRNEVD IQIACSLGML TEEQVQRLAD 

       190        200        210        220        230        240 
MGVHRYNHNL ETARSFFPNV VTTHTWEERW DTLNMVREAG MEVCCGGILG MGETLEQRAE 

       250        260        270        280        290        300 
FAANLAELNP HEVPLNFLNP RPGTPFGDLE VLPASEALKA VAAFRLALPR TMLRFAGGRE 

       310        320        330        340        350 
ITLGDLGAKK GILGGINAVI VGNYLTTLGR PAEADLQLLD DLQMPIKALN ATL 

« Hide

References

[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[2]"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[3]"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000480 Genomic DNA. Translation: ABK73579.1.
CP001663 Genomic DNA. Translation: AFP39576.1. Different initiation.
RefSeqYP_006567871.1. NC_018289.1.
YP_887508.1. NC_008596.1.

3D structure databases

ProteinModelPortalA0QX70.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246196.MSMEG_3194.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK73579; ABK73579; MSMEG_3194.
AFP39576; AFP39576; MSMEI_3112.
GeneID4534990.
KEGGmsg:MSMEI_3112.
msm:MSMEG_3194.
PATRIC18078816. VBIMycSme59918_3156.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMAETHHNID.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycMSME246196:GJ4Y-3194-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_MYCS2
AccessionPrimary (citable) accession number: A0QX70
Secondary accession number(s): I7G8Q7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways