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A0QX70

- BIOB_MYCS2

UniProt

A0QX70 - BIOB_MYCS2

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Protein
Biotin synthase
Gene
bioB, MSMEG_3194, MSMEI_3112
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi89 – 891Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi93 – 931Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi96 – 961Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi132 – 1321Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi165 – 1651Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi224 – 2241Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi294 – 2941Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3194-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:MSMEG_3194, MSMEI_3112
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Biotin synthaseUniRule annotation
PRO_0000381482Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_3194.

Structurei

3D structure databases

ProteinModelPortaliA0QX70.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiETHHNID.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QX70-1 [UniParc]FASTAAdd to Basket

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MFEVFRDLFN PQEDGVTQAA INGTDVLDVA REQVLERGVG LDQAQTLQVL    50
QLPDERLDDL LSLAHEVRMR WCGPDVEVEG IISLKTGGCP EDCHFCSQSG 100
LFASPVRSAW LDVPSLVEAA KQTAKTGATE FCIVAAVRGP DERLLAQVAA 150
GIEAIRNEVD IQIACSLGML TEEQVQRLAD MGVHRYNHNL ETARSFFPNV 200
VTTHTWEERW DTLNMVREAG MEVCCGGILG MGETLEQRAE FAANLAELNP 250
HEVPLNFLNP RPGTPFGDLE VLPASEALKA VAAFRLALPR TMLRFAGGRE 300
ITLGDLGAKK GILGGINAVI VGNYLTTLGR PAEADLQLLD DLQMPIKALN 350
ATL 353
Length:353
Mass (Da):38,396
Last modified:January 9, 2007 - v1
Checksum:iDC0B6115C06BEB0D
GO

Sequence cautioni

The sequence AFP39576.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK73579.1.
CP001663 Genomic DNA. Translation: AFP39576.1. Different initiation.
RefSeqiYP_006567871.1. NC_018289.1.
YP_887508.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73579; ABK73579; MSMEG_3194.
AFP39576; AFP39576; MSMEI_3112.
GeneIDi4534990.
KEGGimsg:MSMEI_3112.
msm:MSMEG_3194.
PATRICi18078816. VBIMycSme59918_3156.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK73579.1 .
CP001663 Genomic DNA. Translation: AFP39576.1 . Different initiation.
RefSeqi YP_006567871.1. NC_018289.1.
YP_887508.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0QX70.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_3194.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK73579 ; ABK73579 ; MSMEG_3194 .
AFP39576 ; AFP39576 ; MSMEI_3112 .
GeneIDi 4534990.
KEGGi msg:MSMEI_3112.
msm:MSMEG_3194.
PATRICi 18078816. VBIMycSme59918_3156.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi ETHHNID.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MSME246196:GJ4Y-3194-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiBIOB_MYCS2
AccessioniPrimary (citable) accession number: A0QX70
Secondary accession number(s): I7G8Q7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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