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Protein

8-amino-7-oxononanoate synthase

Gene

bioF

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.By similarity

Catalytic activityi

Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein].

Cofactori

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26SubstrateBy similarity1
Binding sitei129SubstrateBy similarity1
Binding sitei175Pyridoxal phosphateBy similarity1
Binding sitei345SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
8-amino-7-oxononanoate synthase (EC:2.3.1.47)
Short name:
AONS
Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name:
7-KAP synthase
Short name:
KAPA synthase
8-amino-7-ketopelargonate synthase
Alpha-oxoamine synthase
Gene namesi
Name:bioF
Ordered Locus Names:MSMEG_3189, MSMEI_3107
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003810391 – 3828-amino-7-oxononanoate synthaseAdd BLAST382

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei235N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_3189.

Structurei

Secondary structure

1382
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 24Combined sources11
Helixi52 – 65Combined sources14
Beta strandi73 – 76Combined sources4
Helixi80 – 93Combined sources14
Beta strandi96 – 103Combined sources8
Helixi104 – 115Combined sources12
Beta strandi120 – 125Combined sources6
Turni126 – 128Combined sources3
Helixi129 – 139Combined sources11
Beta strandi141 – 146Combined sources6
Helixi151 – 159Combined sources9
Beta strandi164 – 173Combined sources10
Turni175 – 177Combined sources3
Helixi183 – 192Combined sources10
Beta strandi196 – 200Combined sources5
Turni202 – 207Combined sources6
Helixi215 – 218Combined sources4
Beta strandi227 – 235Combined sources9
Beta strandi242 – 246Combined sources5
Helixi248 – 257Combined sources10
Helixi259 – 262Combined sources4
Helixi269 – 284Combined sources16
Helixi287 – 303Combined sources17
Beta strandi310 – 320Combined sources11
Turni321 – 323Combined sources3
Helixi324 – 333Combined sources10
Beta strandi339 – 341Combined sources3
Turni343 – 345Combined sources3
Beta strandi351 – 356Combined sources6
Helixi363 – 378Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WY7X-ray2.30A/B/C/D1-382[»]
ProteinModelPortaliA0QX65.
SMRiA0QX65.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 105Pyridoxal phosphate bindingBy similarity2
Regioni200 – 203Pyridoxal phosphate bindingBy similarity4
Regioni232 – 235Pyridoxal phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.
HOGENOMiHOG000221021.
KOiK00652.
OMAiENGAYFR.
OrthoDBiPOG091H024U.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QX65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRAGLSPLA WLADIEQRRR AEGLRRELRV RPPVAAELDL ASNDYLGLSQ
60 70 80 90 100
HPDVLDGGVE ALRTWGGGAG GSRLVTGNTE LHEAFEHQLA SFLGAESALV
110 120 130 140 150
FSSGYTANLG ALVALSGPGS LIVSDALSHA SLVDACRLSR ARVVVSPHRD
160 170 180 190 200
VDAVDAALAA RTEERAVVVT ESVFSADGDL APLRDLHAVC RRHGALLLVD
210 220 230 240 250
EAHGLGVRGT RGQGLLHEVG LAGAPDIVMT TTLSKALGSQ GGAVLGPEAV
260 270 280 290 300
RAHLIDTARS FIFDTGLAPA AVGAASAALR VLDAEPQRAR AVLDRAAELA
310 320 330 340 350
TIAGVTEAPV SAVVSVILGD PEIAVGAAAA CLDRGVRVGC FRPPTVPAGT
360 370 380
SRLRLAARAS LTDDEMALAR QVLTDVLATA RA
Length:382
Mass (Da):39,343
Last modified:January 9, 2007 - v1
Checksum:iA1605D7DF40D6111
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73253.1.
CP001663 Genomic DNA. Translation: AFP39571.1.
RefSeqiWP_011728881.1. NZ_CP009494.1.
YP_887503.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73253; ABK73253; MSMEG_3189.
AFP39571; AFP39571; MSMEI_3107.
GeneIDi4537143.
KEGGimsb:LJ00_15855.
msg:MSMEI_3107.
msm:MSMEG_3189.
PATRICi18078806. VBIMycSme59918_3151.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73253.1.
CP001663 Genomic DNA. Translation: AFP39571.1.
RefSeqiWP_011728881.1. NZ_CP009494.1.
YP_887503.1. NC_008596.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WY7X-ray2.30A/B/C/D1-382[»]
ProteinModelPortaliA0QX65.
SMRiA0QX65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3189.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73253; ABK73253; MSMEG_3189.
AFP39571; AFP39571; MSMEI_3107.
GeneIDi4537143.
KEGGimsb:LJ00_15855.
msg:MSMEI_3107.
msm:MSMEG_3189.
PATRICi18078806. VBIMycSme59918_3151.

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.
HOGENOMiHOG000221021.
KOiK00652.
OMAiENGAYFR.
OrthoDBiPOG091H024U.

Enzyme and pathway databases

UniPathwayiUPA00078.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIOF_MYCS2
AccessioniPrimary (citable) accession number: A0QX65
Secondary accession number(s): I7G8Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.