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Protein

8-amino-7-oxononanoate synthase

Gene

bioF

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.By similarity

Catalytic activityi

Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein].

Cofactori

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261SubstrateBy similarity
Binding sitei129 – 1291SubstrateBy similarity
Binding sitei175 – 1751Pyridoxal phosphateBy similarity
Binding sitei345 – 3451SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3189-MONOMER.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
8-amino-7-oxononanoate synthase (EC:2.3.1.47)
Short name:
AONS
Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name:
7-KAP synthase
Short name:
KAPA synthase
8-amino-7-ketopelargonate synthase
Alpha-oxoamine synthase
Gene namesi
Name:bioF
Ordered Locus Names:MSMEG_3189, MSMEI_3107
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3823828-amino-7-oxononanoate synthasePRO_0000381039Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei235 – 2351N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_3189.

Structurei

Secondary structure

1
382
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 2411Combined sources
Helixi52 – 6514Combined sources
Beta strandi73 – 764Combined sources
Helixi80 – 9314Combined sources
Beta strandi96 – 1038Combined sources
Helixi104 – 11512Combined sources
Beta strandi120 – 1256Combined sources
Turni126 – 1283Combined sources
Helixi129 – 13911Combined sources
Beta strandi141 – 1466Combined sources
Helixi151 – 1599Combined sources
Beta strandi164 – 17310Combined sources
Turni175 – 1773Combined sources
Helixi183 – 19210Combined sources
Beta strandi196 – 2005Combined sources
Turni202 – 2076Combined sources
Helixi215 – 2184Combined sources
Beta strandi227 – 2359Combined sources
Beta strandi242 – 2465Combined sources
Helixi248 – 25710Combined sources
Helixi259 – 2624Combined sources
Helixi269 – 28416Combined sources
Helixi287 – 30317Combined sources
Beta strandi310 – 32011Combined sources
Turni321 – 3233Combined sources
Helixi324 – 33310Combined sources
Beta strandi339 – 3413Combined sources
Turni343 – 3453Combined sources
Beta strandi351 – 3566Combined sources
Helixi363 – 37816Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WY7X-ray2.30A/B/C/D1-382[»]
ProteinModelPortaliA0QX65.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 1052Pyridoxal phosphate bindingBy similarity
Regioni200 – 2034Pyridoxal phosphate bindingBy similarity
Regioni232 – 2354Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.
HOGENOMiHOG000221021.
KOiK00652.
OMAiENGAYFR.
OrthoDBiPOG091H024U.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QX65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRAGLSPLA WLADIEQRRR AEGLRRELRV RPPVAAELDL ASNDYLGLSQ
60 70 80 90 100
HPDVLDGGVE ALRTWGGGAG GSRLVTGNTE LHEAFEHQLA SFLGAESALV
110 120 130 140 150
FSSGYTANLG ALVALSGPGS LIVSDALSHA SLVDACRLSR ARVVVSPHRD
160 170 180 190 200
VDAVDAALAA RTEERAVVVT ESVFSADGDL APLRDLHAVC RRHGALLLVD
210 220 230 240 250
EAHGLGVRGT RGQGLLHEVG LAGAPDIVMT TTLSKALGSQ GGAVLGPEAV
260 270 280 290 300
RAHLIDTARS FIFDTGLAPA AVGAASAALR VLDAEPQRAR AVLDRAAELA
310 320 330 340 350
TIAGVTEAPV SAVVSVILGD PEIAVGAAAA CLDRGVRVGC FRPPTVPAGT
360 370 380
SRLRLAARAS LTDDEMALAR QVLTDVLATA RA
Length:382
Mass (Da):39,343
Last modified:January 9, 2007 - v1
Checksum:iA1605D7DF40D6111
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73253.1.
CP001663 Genomic DNA. Translation: AFP39571.1.
RefSeqiWP_011728881.1. NZ_CP009494.1.
YP_887503.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73253; ABK73253; MSMEG_3189.
AFP39571; AFP39571; MSMEI_3107.
GeneIDi4537143.
KEGGimsb:LJ00_15855.
msg:MSMEI_3107.
msm:MSMEG_3189.
PATRICi18078806. VBIMycSme59918_3151.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73253.1.
CP001663 Genomic DNA. Translation: AFP39571.1.
RefSeqiWP_011728881.1. NZ_CP009494.1.
YP_887503.1. NC_008596.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WY7X-ray2.30A/B/C/D1-382[»]
ProteinModelPortaliA0QX65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3189.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73253; ABK73253; MSMEG_3189.
AFP39571; AFP39571; MSMEI_3107.
GeneIDi4537143.
KEGGimsb:LJ00_15855.
msg:MSMEI_3107.
msm:MSMEG_3189.
PATRICi18078806. VBIMycSme59918_3151.

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.
HOGENOMiHOG000221021.
KOiK00652.
OMAiENGAYFR.
OrthoDBiPOG091H024U.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciMSME246196:GJ4Y-3189-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIOF_MYCS2
AccessioniPrimary (citable) accession number: A0QX65
Secondary accession number(s): I7G8Q0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.