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Protein

8-amino-7-oxononanoate synthase

Gene

bioF

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.By similarity

Catalytic activityi

Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein].

Cofactori

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26SubstrateBy similarity1
Binding sitei129SubstrateBy similarity1
Binding sitei175Pyridoxal phosphateBy similarity1
Binding sitei345SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processBiotin biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-3191-MONOMER
UniPathwayiUPA00078

Names & Taxonomyi

Protein namesi
Recommended name:
8-amino-7-oxononanoate synthase (EC:2.3.1.47)
Short name:
AONS
Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name:
7-KAP synthase
Short name:
KAPA synthase
8-amino-7-ketopelargonate synthase
Alpha-oxoamine synthase
Gene namesi
Name:bioF
Ordered Locus Names:MSMEG_3189, MSMEI_3107
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003810391 – 3828-amino-7-oxononanoate synthaseAdd BLAST382

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei235N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PRIDEiA0QX65

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_3189

Structurei

Secondary structure

1382
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 24Combined sources11
Helixi52 – 65Combined sources14
Beta strandi73 – 76Combined sources4
Helixi80 – 93Combined sources14
Beta strandi96 – 103Combined sources8
Helixi104 – 115Combined sources12
Beta strandi120 – 125Combined sources6
Turni126 – 128Combined sources3
Helixi129 – 139Combined sources11
Beta strandi141 – 146Combined sources6
Helixi151 – 159Combined sources9
Beta strandi164 – 173Combined sources10
Turni175 – 177Combined sources3
Helixi183 – 192Combined sources10
Beta strandi196 – 200Combined sources5
Turni202 – 207Combined sources6
Helixi215 – 218Combined sources4
Beta strandi227 – 235Combined sources9
Beta strandi242 – 246Combined sources5
Helixi248 – 257Combined sources10
Helixi259 – 262Combined sources4
Helixi269 – 284Combined sources16
Helixi287 – 303Combined sources17
Beta strandi310 – 320Combined sources11
Turni321 – 323Combined sources3
Helixi324 – 333Combined sources10
Beta strandi339 – 341Combined sources3
Turni343 – 345Combined sources3
Beta strandi351 – 356Combined sources6
Helixi363 – 378Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WY7X-ray2.30A/B/C/D1-382[»]
ProteinModelPortaliA0QX65
SMRiA0QX65
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 105Pyridoxal phosphate bindingBy similarity2
Regioni200 – 203Pyridoxal phosphate bindingBy similarity4
Regioni232 – 235Pyridoxal phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107EEK Bacteria
COG0156 LUCA
HOGENOMiHOG000221021
KOiK00652
OMAiFGTIGEN
OrthoDBiPOG091H024U

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR001917 Aminotrans_II_pyridoxalP_BS
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00599 AA_TRANSFER_CLASS_2, 1 hit

Sequencei

Sequence statusi: Complete.

A0QX65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRAGLSPLA WLADIEQRRR AEGLRRELRV RPPVAAELDL ASNDYLGLSQ
60 70 80 90 100
HPDVLDGGVE ALRTWGGGAG GSRLVTGNTE LHEAFEHQLA SFLGAESALV
110 120 130 140 150
FSSGYTANLG ALVALSGPGS LIVSDALSHA SLVDACRLSR ARVVVSPHRD
160 170 180 190 200
VDAVDAALAA RTEERAVVVT ESVFSADGDL APLRDLHAVC RRHGALLLVD
210 220 230 240 250
EAHGLGVRGT RGQGLLHEVG LAGAPDIVMT TTLSKALGSQ GGAVLGPEAV
260 270 280 290 300
RAHLIDTARS FIFDTGLAPA AVGAASAALR VLDAEPQRAR AVLDRAAELA
310 320 330 340 350
TIAGVTEAPV SAVVSVILGD PEIAVGAAAA CLDRGVRVGC FRPPTVPAGT
360 370 380
SRLRLAARAS LTDDEMALAR QVLTDVLATA RA
Length:382
Mass (Da):39,343
Last modified:January 9, 2007 - v1
Checksum:iA1605D7DF40D6111
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK73253.1
CP001663 Genomic DNA Translation: AFP39571.1
RefSeqiWP_011728881.1, NZ_CP009494.1
YP_887503.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK73253; ABK73253; MSMEG_3189
AFP39571; AFP39571; MSMEI_3107
GeneIDi4537143
KEGGimsb:LJ00_15855
msg:MSMEI_3107
msm:MSMEG_3189
PATRICifig|246196.19.peg.3151

Similar proteinsi

Entry informationi

Entry nameiBIOF_MYCS2
AccessioniPrimary (citable) accession number: A0QX65
Secondary accession number(s): I7G8Q0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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