A0QX61 (A0QX61_MYCS2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Name:	treZ EMBL ABK71872.1
Ordered Locus Names:	MSMEG_3184

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) EMBL ABK71872.1

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 EMBL ABK71872.1 Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	244	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	281	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	377	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A0QX61 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 904D07094545E477	FASTA	583	64,618
	10 20 30 40 50 60 MTEFAVWAPL PDVVRLDFEG TLHDMTRSDD GWWRAEVECA DDARYGFVLD DDPKVLPDPR 70 80 90 100 110 120 SARQPDGVHE RSQLWRPRPD AWTDADWPGR SIEGRVIYEL HIGTFTPEGT FDSAIEKLDY 130 140 150 160 170 180 LVDLGVDFVE LMPVNAFGGT HGWGYDGVLW YAVHEPYGGP DGLIRFVDAC HTRGLGVLID 190 200 210 220 230 240 AVFNHLGPSG NYLPKFAPYL SAGSNPWGAS INLSDADADE VRTYILECAL RWMRDFHADG 250 260 270 280 290 300 LRLDAVHALM DNTAIHILEE LSAETDRLAE ELGRPLSLIA ESDLNDPRLI TPRDRGGYGM 310 320 330 340 350 360 TAQWDDDIHH AIHTAVSGER QGYYADFGSM ETLATTLKNG YFHAGTYSSF RHRRHGRPLD 370 380 390 400 410 420 TATIPGSRLL AYTLTHDQVG NRAVGDRPSQ RLTTGQLAVK AALALGSPYT AMLFMGEEWG 430 440 450 460 470 480 SSSPFQFFSS HPEPELARAT AEGRKAEFAG HGWDADEIPD PQDPATFERS KLKWDELDRG 490 500 510 520 530 540 EHAQLLGIYR ELIELRRTEP ALADMWLDHL RVDFDEDDRW IVLYRGDLAI ACNLNAEPVT 550 560 570 580 IGISGDLVLA SSTPEARPDG TVLAGHSFAI LRLVNSASAA GPG

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK71872.1.
RefSeq	YP_887499.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QX61.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QX61.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000046471; EBMYCP00000044808; EBMYCG00000046466.
GeneID	4536822.
GenomeReviews	Gene locus MSMEG_3184 in contig CP000480_GR.
KEGG	msm:MSMEG_3184.
NMPDR	fig|246196.1.peg.3191.
PATRIC	18078794. VBIMycSme59918_3145.
TIGR	MSMEG_3184.
Phylogenomic databases
eggNOG	COG0296.
GeneTree	EBGT00050000016657.
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK799816.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0QX61_MYCS2

Accession

Primary (citable) accession number: A0QX61

Entry history

Integrated into UniProtKB/TrEMBL:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)