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Protein

Lipoprotein signal peptidase

Gene

lspA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This protein specifically catalyzes the removal of signal peptides from prolipoproteins.UniRule annotation1 Publication

Catalytic activityi

Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.UniRule annotation

Pathwayi: lipoprotein biosynthesis (signal peptide cleavage)

This protein is involved in the pathway lipoprotein biosynthesis (signal peptide cleavage), which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway lipoprotein biosynthesis (signal peptide cleavage) and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei129UniRule annotation1
Active sitei158UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-3176-MONOMER
UniPathwayiUPA00665

Protein family/group databases

MEROPSiA08.001

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein signal peptidaseUniRule annotation (EC:3.4.23.36UniRule annotation)
Alternative name(s):
Prolipoprotein signal peptidaseUniRule annotation
Signal peptidase IIUniRule annotation
Gene namesi
Name:lspAUniRule annotation
Ordered Locus Names:MSMEG_3174, MSMEI_3092
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei31 – 51HelicalUniRule annotationAdd BLAST21
Transmembranei84 – 104HelicalUniRule annotationAdd BLAST21
Transmembranei108 – 128HelicalUniRule annotationAdd BLAST21
Transmembranei156 – 176HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No processing of prolipoprotein LppX and synthetic prolipoprotein constructs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004348931 – 235Lipoprotein signal peptidaseAdd BLAST235

Keywords - PTMi

Lipoprotein

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_3174

Structurei

3D structure databases

ProteinModelPortaliA0QX51
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A8 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107ZIW Bacteria
COG0597 LUCA
HOGENOMiHOG000096992
KOiK03101
OMAiRAHNTGA
OrthoDBiPOG091H059F

Family and domain databases

HAMAPiMF_00161 LspA, 1 hit
InterProiView protein in InterPro
IPR001872 Peptidase_A8
PANTHERiPTHR33695 PTHR33695, 1 hit
PfamiView protein in Pfam
PF01252 Peptidase_A8, 1 hit
PRINTSiPR00781 LIPOSIGPTASE
TIGRFAMsiTIGR00077 lspA, 1 hit
PROSITEiView protein in PROSITE
PS00855 SPASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

A0QX51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDETSGPAE PVTDAPGDAE SPAQPKRRLR LLLTVAAVVL FLDVVTKVLA
60 70 80 90 100
VRLLTPGQPV SIIGDTVTWT LVRNSGAAFS MATGYTWVLT LVATGVVIGI
110 120 130 140 150
IWMGRRLVSP WWALGLGLIL GGATGNLVDR FFRSPGPLRG HVVDFFSVGW
160 170 180 190 200
WPVFNVADPS VVGGAILLVA LSLFGFDFDT VGRRRPGEDA EPSAGASDST
210 220 230
PEAPAADGPD KPAGPVGPED AAEESKTVGH QAEPS
Length:235
Mass (Da):24,535
Last modified:January 9, 2007 - v1
Checksum:i15F770F5926120B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK75519.1
CP001663 Genomic DNA Translation: AFP39556.1
RefSeqiWP_011728868.1, NZ_CP009494.1
YP_887489.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK75519; ABK75519; MSMEG_3174
AFP39556; AFP39556; MSMEI_3092
GeneIDi4532001
KEGGimsb:LJ00_15780
msg:MSMEI_3092
msm:MSMEG_3174
PATRICifig|246196.19.peg.3135

Similar proteinsi

Entry informationi

Entry nameiLSPA_MYCS2
AccessioniPrimary (citable) accession number: A0QX51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 9, 2015
Last sequence update: January 9, 2007
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health