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Protein

Lipoprotein signal peptidase

Gene

lspA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein specifically catalyzes the removal of signal peptides from prolipoproteins.UniRule annotation1 Publication

Catalytic activityi

Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.UniRule annotation

Pathwayi: lipoprotein biosynthesis (signal peptide cleavage)

This protein is involved in the pathway lipoprotein biosynthesis (signal peptide cleavage), which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway lipoprotein biosynthesis (signal peptide cleavage) and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei129 – 1291UniRule annotation
Active sitei158 – 1581UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3174-MONOMER.
UniPathwayiUPA00665.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein signal peptidaseUniRule annotation (EC:3.4.23.36UniRule annotation)
Alternative name(s):
Prolipoprotein signal peptidaseUniRule annotation
Signal peptidase IIUniRule annotation
Gene namesi
Name:lspAUniRule annotation
Ordered Locus Names:MSMEG_3174, MSMEI_3092
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei31 – 5121HelicalUniRule annotationAdd
BLAST
Transmembranei84 – 10421HelicalUniRule annotationAdd
BLAST
Transmembranei108 – 12821HelicalUniRule annotationAdd
BLAST
Transmembranei156 – 17621HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No processing of prolipoprotein LppX and synthetic prolipoprotein constructs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 235235Lipoprotein signal peptidasePRO_0000434893Add
BLAST

Keywords - PTMi

Lipoprotein

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_3174.

Structurei

3D structure databases

ProteinModelPortaliA0QX51.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A8 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0597.
HOGENOMiHOG000096992.
KOiK03101.
OMAiVAKLEHH.
OrthoDBiPOG091H059F.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QX51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDETSGPAE PVTDAPGDAE SPAQPKRRLR LLLTVAAVVL FLDVVTKVLA
60 70 80 90 100
VRLLTPGQPV SIIGDTVTWT LVRNSGAAFS MATGYTWVLT LVATGVVIGI
110 120 130 140 150
IWMGRRLVSP WWALGLGLIL GGATGNLVDR FFRSPGPLRG HVVDFFSVGW
160 170 180 190 200
WPVFNVADPS VVGGAILLVA LSLFGFDFDT VGRRRPGEDA EPSAGASDST
210 220 230
PEAPAADGPD KPAGPVGPED AAEESKTVGH QAEPS
Length:235
Mass (Da):24,535
Last modified:January 9, 2007 - v1
Checksum:i15F770F5926120B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75519.1.
CP001663 Genomic DNA. Translation: AFP39556.1.
RefSeqiWP_011728868.1. NZ_CP009494.1.
YP_887489.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK75519; ABK75519; MSMEG_3174.
AFP39556; AFP39556; MSMEI_3092.
GeneIDi4532001.
KEGGimsb:LJ00_15780.
msg:MSMEI_3092.
msm:MSMEG_3174.
PATRICi18078774. VBIMycSme59918_3135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75519.1.
CP001663 Genomic DNA. Translation: AFP39556.1.
RefSeqiWP_011728868.1. NZ_CP009494.1.
YP_887489.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QX51.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK75519; ABK75519; MSMEG_3174.
AFP39556; AFP39556; MSMEI_3092.
GeneIDi4532001.
KEGGimsb:LJ00_15780.
msg:MSMEI_3092.
msm:MSMEG_3174.
PATRICi18078774. VBIMycSme59918_3135.

Phylogenomic databases

eggNOGiCOG0597.
HOGENOMiHOG000096992.
KOiK03101.
OMAiVAKLEHH.
OrthoDBiPOG091H059F.

Enzyme and pathway databases

UniPathwayiUPA00665.
BioCyciMSME246196:GJ4Y-3174-MONOMER.

Family and domain databases

HAMAPiMF_00161. LspA. 1 hit.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PANTHERiPTHR33695:SF1. PTHR33695:SF1. 1 hit.
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSPA_MYCS2
AccessioniPrimary (citable) accession number: A0QX51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 9, 2015
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.