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Protein

Aconitate hydratase A

Gene

acnA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. The apo form of AcnA functions as a RNA-binding regulatory protein. Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate.By similarity

Catalytic activityi

Citrate = isocitrate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA)
  2. Aconitate hydratase A (acnA)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Pathwayi: propanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi478Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi544Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi547Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Iron, Iron-sulfur, Metal-binding, RNA-binding

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.
UPA00946.

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase ABy similarity (EC:4.2.1.3By similarity)
Short name:
ACNBy similarity
Short name:
AconitaseBy similarity
Alternative name(s):
(2R,3S)-2-methylisocitrate dehydrataseBy similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydrataseBy similarity
Iron-responsive protein-likeBy similarity
Short name:
IRP-likeBy similarity
Probable 2-methyl-cis-aconitate hydrataseBy similarity (EC:4.2.1.99By similarity)
RNA-binding proteinBy similarity
Gene namesi
Name:acnA
Synonyms:acn
Ordered Locus Names:MSMEG_3143, MSMEI_3062
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003968181 – 943Aconitate hydratase AAdd BLAST943

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki394Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_3143.

Structurei

3D structure databases

ProteinModelPortaliA0QX20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Phylogenomic databases

eggNOGiENOG4107QM5. Bacteria.
COG1048. LUCA.
HOGENOMiHOG000025704.
KOiK01681.
OMAiENLAKWG.
OrthoDBiPOG091H08NL.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 4 hits.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 2 hits.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QX20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSENTGKSS LNSFGARDTL TVGDQSYEIY RLNAVPGTEK LPYSLKVLAE
60 70 80 90 100
NLLRTEDGAN ITKDHIEAIA NWDPNAEPSI EIQFTPARVI MQDFTGVPCI
110 120 130 140 150
VDLATMREAV AALGGDPNKV NPLAPAELVI DHSVILDVFG NASAFERNVE
160 170 180 190 200
LEYERNAERY QFLRWGQGAF DDFKVVPPGT GIVHQVNIEY LARTVMVRDG
210 220 230 240 250
VAYPDTCVGT DSHTTMVNGL GVLGWGVGGI EAEAAMLGQP VSMLIPRVVG
260 270 280 290 300
FKLSGEIKPG VTATDVVLTV TDMLRRHGVV GKFVEFYGKG VAEVPLANRA
310 320 330 340 350
TLGNMSPEFG STAAIFPIDE ETINYLRLTG RTDEQLALVE AYAKAQGMWH
360 370 380 390 400
DPEREPVFSE YLELDLSTVV PSISGPKRPQ DRIELTDAKN AFRKDIHNYV
410 420 430 440 450
EQNHPTPETK LDEAVEESFP ASDPVSLSFA DDGAPDMRPS AANGATGRPT
460 470 480 490 500
NPVLVHSEER GDFVLDHGAV VVAGITSCTN TSNPSVMLGA ALLAKKAVEK
510 520 530 540 550
GLTTKPWVKT NMAPGSQVVT DYYNKAGLWP YLEKLGYYLG GYGCTTCIGN
560 570 580 590 600
TGPLPEEISK AINDNDLAVT AVLSGNRNFE GRISPDVKMN YLASPPLVIA
610 620 630 640 650
YGIAGTMDFD FESDPLGQDS EGNDVFLRDI WPSAAEIEET IASSINREMF
660 670 680 690 700
TESYADVFKG DDRWRSLPTP EGDTFEWDPA STYVRKAPYF DGMPAEPEPV
710 720 730 740 750
SDIKGARVLA LLGDSVTTDH ISPAGAIKPG TPAAQYLDAN GVERKDYNSL
760 770 780 790 800
GSRRGNHEVM IRGTFANIRL RNQLLDDVSG GYTRDFTQPG GPQAFIYDAS
810 820 830 840 850
ENYKKAGIPL VVLGGKEYGS GSSRDWAAKG TVLLGVKAVI TESFERIHRS
860 870 880 890 900
NLIGMGVIPL QFPAGESAAS LKLDGTETYD IEGIEELNSG KTPKTVHVTA
910 920 930 940
TKEDGSKVEF DAVVRIDTPG EADYYRNGGI LQYVLRNMLK SSK
Length:943
Mass (Da):102,171
Last modified:January 9, 2007 - v1
Checksum:iBD10F939FA09F201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ866855 Genomic DNA. Translation: ABJ96316.1.
CP000480 Genomic DNA. Translation: ABK73859.1.
CP001663 Genomic DNA. Translation: AFP39526.1.
RefSeqiWP_011728843.1. NZ_CP009494.1.
YP_887458.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73859; ABK73859; MSMEG_3143.
AFP39526; AFP39526; MSMEI_3062.
GeneIDi4536477.
KEGGimsb:LJ00_15630.
msg:MSMEI_3062.
msm:MSMEG_3143.
PATRICi18078710. VBIMycSme59918_3103.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ866855 Genomic DNA. Translation: ABJ96316.1.
CP000480 Genomic DNA. Translation: ABK73859.1.
CP001663 Genomic DNA. Translation: AFP39526.1.
RefSeqiWP_011728843.1. NZ_CP009494.1.
YP_887458.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QX20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3143.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73859; ABK73859; MSMEG_3143.
AFP39526; AFP39526; MSMEI_3062.
GeneIDi4536477.
KEGGimsb:LJ00_15630.
msg:MSMEI_3062.
msm:MSMEG_3143.
PATRICi18078710. VBIMycSme59918_3103.

Phylogenomic databases

eggNOGiENOG4107QM5. Bacteria.
COG1048. LUCA.
HOGENOMiHOG000025704.
KOiK01681.
OMAiENLAKWG.
OrthoDBiPOG091H08NL.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.
UPA00946.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 4 hits.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 2 hits.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACNA_MYCS2
AccessioniPrimary (citable) accession number: A0QX20
Secondary accession number(s): A4ZHS6, I7G8K0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.