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Protein

Aconitate hydratase A

Gene

acnA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. The apo form of AcnA functions as a RNA-binding regulatory protein. Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate.By similarity

Catalytic activityi

Citrate = isocitrate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA), Citrate synthase (gltA2)
  2. Aconitate hydratase A (acnA)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Pathwayi: propanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi478Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi544Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi547Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, RNA-binding
Biological processTricarboxylic acid cycle
LigandIron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-3145-MONOMER
UniPathwayiUPA00223; UER00718
UPA00946

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase ABy similarity (EC:4.2.1.3By similarity)
Short name:
ACNBy similarity
Short name:
AconitaseBy similarity
Alternative name(s):
(2R,3S)-2-methylisocitrate dehydrataseBy similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydrataseBy similarity
Iron-responsive protein-likeBy similarity
Short name:
IRP-likeBy similarity
Probable 2-methyl-cis-aconitate hydrataseBy similarity (EC:4.2.1.99By similarity)
RNA-binding proteinBy similarity
Gene namesi
Name:acnA
Synonyms:acn
Ordered Locus Names:MSMEG_3143, MSMEI_3062
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003968181 – 943Aconitate hydratase AAdd BLAST943

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki394Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_3143

Structurei

3D structure databases

ProteinModelPortaliA0QX20
SMRiA0QX20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Phylogenomic databases

eggNOGiENOG4107QM5 Bacteria
COG1048 LUCA
HOGENOMiHOG000025704
KOiK01681
OMAiMRIIPPG
OrthoDBiPOG091H08NL

Family and domain databases

Gene3Di3.20.19.10, 1 hit
InterProiView protein in InterPro
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR015928 Aconitase/3IPM_dehydase_swvl
IPR006249 Aconitase/IRP2
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR000573 AconitaseA/IPMdHydase_ssu_swvl
PANTHERiPTHR11670 PTHR11670, 1 hit
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PF00694 Aconitase_C, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 2 hits
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

A0QX20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSENTGKSS LNSFGARDTL TVGDQSYEIY RLNAVPGTEK LPYSLKVLAE
60 70 80 90 100
NLLRTEDGAN ITKDHIEAIA NWDPNAEPSI EIQFTPARVI MQDFTGVPCI
110 120 130 140 150
VDLATMREAV AALGGDPNKV NPLAPAELVI DHSVILDVFG NASAFERNVE
160 170 180 190 200
LEYERNAERY QFLRWGQGAF DDFKVVPPGT GIVHQVNIEY LARTVMVRDG
210 220 230 240 250
VAYPDTCVGT DSHTTMVNGL GVLGWGVGGI EAEAAMLGQP VSMLIPRVVG
260 270 280 290 300
FKLSGEIKPG VTATDVVLTV TDMLRRHGVV GKFVEFYGKG VAEVPLANRA
310 320 330 340 350
TLGNMSPEFG STAAIFPIDE ETINYLRLTG RTDEQLALVE AYAKAQGMWH
360 370 380 390 400
DPEREPVFSE YLELDLSTVV PSISGPKRPQ DRIELTDAKN AFRKDIHNYV
410 420 430 440 450
EQNHPTPETK LDEAVEESFP ASDPVSLSFA DDGAPDMRPS AANGATGRPT
460 470 480 490 500
NPVLVHSEER GDFVLDHGAV VVAGITSCTN TSNPSVMLGA ALLAKKAVEK
510 520 530 540 550
GLTTKPWVKT NMAPGSQVVT DYYNKAGLWP YLEKLGYYLG GYGCTTCIGN
560 570 580 590 600
TGPLPEEISK AINDNDLAVT AVLSGNRNFE GRISPDVKMN YLASPPLVIA
610 620 630 640 650
YGIAGTMDFD FESDPLGQDS EGNDVFLRDI WPSAAEIEET IASSINREMF
660 670 680 690 700
TESYADVFKG DDRWRSLPTP EGDTFEWDPA STYVRKAPYF DGMPAEPEPV
710 720 730 740 750
SDIKGARVLA LLGDSVTTDH ISPAGAIKPG TPAAQYLDAN GVERKDYNSL
760 770 780 790 800
GSRRGNHEVM IRGTFANIRL RNQLLDDVSG GYTRDFTQPG GPQAFIYDAS
810 820 830 840 850
ENYKKAGIPL VVLGGKEYGS GSSRDWAAKG TVLLGVKAVI TESFERIHRS
860 870 880 890 900
NLIGMGVIPL QFPAGESAAS LKLDGTETYD IEGIEELNSG KTPKTVHVTA
910 920 930 940
TKEDGSKVEF DAVVRIDTPG EADYYRNGGI LQYVLRNMLK SSK
Length:943
Mass (Da):102,171
Last modified:January 9, 2007 - v1
Checksum:iBD10F939FA09F201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ866855 Genomic DNA Translation: ABJ96316.1
CP000480 Genomic DNA Translation: ABK73859.1
CP001663 Genomic DNA Translation: AFP39526.1
RefSeqiWP_011728843.1, NZ_CP009494.1
YP_887458.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK73859; ABK73859; MSMEG_3143
AFP39526; AFP39526; MSMEI_3062
GeneIDi4536477
KEGGimsb:LJ00_15630
msg:MSMEI_3062
msm:MSMEG_3143
PATRICifig|246196.19.peg.3103

Similar proteinsi

Entry informationi

Entry nameiACNA_MYCS2
AccessioniPrimary (citable) accession number: A0QX20
Secondary accession number(s): A4ZHS6, I7G8K0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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