A0QX20 (ACON_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aconitate hydratase
EC=4.2.1.3

Gene names

Name:	acnA
Synonyms:	acn
Ordered Locus Names:	MSMEG_3143, MSMEI_3062

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	943 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the isomerization of citrate to isocitrate via cis-aconitate By similarity.
Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	Iron Iron-sulfur Metal-binding
Molecular function	Lyase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	citrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW isocitrate hydro-lyase (cis-aconitate-forming) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 943	943	Aconitate hydratase		PRO_0000396818
Sites
	Metal binding	478	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	544	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	547	1	Iron-sulfur (4Fe-4S) By similarity
Amino acid modifications
	Cross-link	394		Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sequences

	Sequence		Length	Mass (Da)
	A0QX20 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: BD10F939FA09F201	FASTA	943	102,171
	10 20 30 40 50 60 MSSENTGKSS LNSFGARDTL TVGDQSYEIY RLNAVPGTEK LPYSLKVLAE NLLRTEDGAN 70 80 90 100 110 120 ITKDHIEAIA NWDPNAEPSI EIQFTPARVI MQDFTGVPCI VDLATMREAV AALGGDPNKV 130 140 150 160 170 180 NPLAPAELVI DHSVILDVFG NASAFERNVE LEYERNAERY QFLRWGQGAF DDFKVVPPGT 190 200 210 220 230 240 GIVHQVNIEY LARTVMVRDG VAYPDTCVGT DSHTTMVNGL GVLGWGVGGI EAEAAMLGQP 250 260 270 280 290 300 VSMLIPRVVG FKLSGEIKPG VTATDVVLTV TDMLRRHGVV GKFVEFYGKG VAEVPLANRA 310 320 330 340 350 360 TLGNMSPEFG STAAIFPIDE ETINYLRLTG RTDEQLALVE AYAKAQGMWH DPEREPVFSE 370 380 390 400 410 420 YLELDLSTVV PSISGPKRPQ DRIELTDAKN AFRKDIHNYV EQNHPTPETK LDEAVEESFP 430 440 450 460 470 480 ASDPVSLSFA DDGAPDMRPS AANGATGRPT NPVLVHSEER GDFVLDHGAV VVAGITSCTN 490 500 510 520 530 540 TSNPSVMLGA ALLAKKAVEK GLTTKPWVKT NMAPGSQVVT DYYNKAGLWP YLEKLGYYLG 550 560 570 580 590 600 GYGCTTCIGN TGPLPEEISK AINDNDLAVT AVLSGNRNFE GRISPDVKMN YLASPPLVIA 610 620 630 640 650 660 YGIAGTMDFD FESDPLGQDS EGNDVFLRDI WPSAAEIEET IASSINREMF TESYADVFKG 670 680 690 700 710 720 DDRWRSLPTP EGDTFEWDPA STYVRKAPYF DGMPAEPEPV SDIKGARVLA LLGDSVTTDH 730 740 750 760 770 780 ISPAGAIKPG TPAAQYLDAN GVERKDYNSL GSRRGNHEVM IRGTFANIRL RNQLLDDVSG 790 800 810 820 830 840 GYTRDFTQPG GPQAFIYDAS ENYKKAGIPL VVLGGKEYGS GSSRDWAAKG TVLLGVKAVI 850 860 870 880 890 900 TESFERIHRS NLIGMGVIPL QFPAGESAAS LKLDGTETYD IEGIEELNSG KTPKTVHVTA 910 920 930 940 TKEDGSKVEF DAVVRIDTPG EADYYRNGGI LQYVLRNMLK SSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Expansion of the mycobacterial 'PUPylome'." Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V., Barry C.E. III, Bark S., Dorrestein P.C. Mol. Biosyst. 6:376-385(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PUPYLATION AT LYS-394, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ866855 Genomic DNA. Translation: ABJ96316.1. CP000480 Genomic DNA. Translation: ABK73859.1. CP001663 Genomic DNA. Translation: AFP39526.1.
RefSeq	YP_006567821.1. NC_018289.1. YP_887458.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QX20.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_3143.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK73859; ABK73859; MSMEG_3143.
GeneID	13425429. 4536477.
KEGG	msg:MSMEI_3062. msm:MSMEG_3143.
PATRIC	18078710. VBIMycSme59918_3103.
Phylogenomic databases
eggNOG	COG1048.
HOGENOM	HOG000025704.
KO	K01681.
OMA	YSKAQGM.
ProtClustDB	CLSK781226.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-3143-MONOMER.
UniPathway	UPA00223; UER00718.
Family and domain databases
Gene3D	3.20.19.10. 1 hit. 3.30.499.10. 3 hits. 3.40.1060.10. 1 hit.
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR015937. Acoase/IPM_deHydtase. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
PANTHER	PTHR11670. PTHR11670. 1 hit. PTHR11670:SF1. PTHR11670:SF1. 1 hit.
Pfam	PF00330. Aconitase. 2 hits. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit. SSF53732. Aconitase_N. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_MYCS2

Accession

Primary (citable) accession number: A0QX20
Secondary accession number(s): A4ZHS6, I7G8K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program