Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A0QWX4 (CAPP_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxylase

Short name=PEPC
Short name=PEPCase
EC=4.1.1.31
Gene names
Name:ppc
Ordered Locus Names:MSMEG_3097, MSMEI_3019
OrganismMycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]
Taxonomic identifier246196 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length933 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP-Rule MF_00595

Catalytic activity

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_00595

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00595

Sequence similarities

Belongs to the PEPCase type 1 family.

Ontologies

Keywords
   Biological processCarbon dioxide fixation
   LigandMagnesium
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbon fixation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

oxaloacetate metabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: InterPro

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 933933Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00595
PRO_1000025566

Sites

Active site1611 By similarity
Active site5931 By similarity

Sequences

Sequence LengthMass (Da)Tools
A0QWX4 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 33F2EAC46B98F7D8

FASTA933103,351
        10         20         30         40         50         60 
MADSNDTALE PFGSVQRTHI GREASEPMRE DIRLLGAILG DTVREQNGEE VFDLVERARV 

        70         80         90        100        110        120 
ESFRVRRSEI DRSELADMFS GVDAHQAIPV IRAFTHFALL ANVAEDIHRE RRRAVHVAAG 

       130        140        150        160        170        180 
KPPQDSSLAA TYRKLDAADL DVDKVADTLT GALVSPVITA HPTETRRRTV FDTQHRITEL 

       190        200        210        220        230        240 
MRLRLHGHTR TDDNRDIETE LRRHILTLWQ TALIRLSRLK ISDEIETGLR YYEAAFFDVI 

       250        260        270        280        290        300 
PQVNAEVRDA LRKRWPDAKL LEEPILRPGS WIGGDRDGNP NVTPEVVRHA TGRAAYVALA 

       310        320        330        340        350        360 
HYFEQITALE QELSMSARLV KVTPALAALA DACHEPARAD EPYRRALRVI HARLTSTARE 

       370        380        390        400        410        420 
ILDEQPEHGL DLGLPRYQTP AEFLADLDAV DGSLRANGSR VLADDRLGRL REAVRVFGFH 

       430        440        450        460        470        480 
LSGLDMRQNS DVHEEVVAEL LAWAGVHPDY TSLSEPQRVE LLAAEIATRR PLIREGAELS 

       490        500        510        520        530        540 
ELAQKELGIV AAAARAVKVF GPQAVPNYII SMCQSVSDML EAAVLLKEAG LLDISGSTPY 

       550        560        570        580        590        600 
APVGVVPLFE TIDDLQRGSS ILEAALDLPE YRTMVDARDG HQEVMLGYSD SNKDGGYLAA 

       610        620        630        640        650        660 
NWALYRAELD LVESARKTGI RLRLFHGRGG TVGRGGGPSY DAILAQPPGA VKGSLRITEQ 

       670        680        690        700        710        720 
GEVIAAKYAE PRIAHRNLET LLAATLEASL LDVEGLGEEA EPAYQVLDEL AALAQRAYSE 

       730        740        750        760        770        780 
LVHETPGFVE YFKTSTPVSE IGALNIGSRP TSRKPTTSIA DLRAIPWVLA WSQSRVMLPG 

       790        800        810        820        830        840 
WYGTGSAFEN WIGTDPDGAR LRVLQDLYAR WPFFRTVLSN MAQVLAKADM GLAARYSELV 

       850        860        870        880        890        900 
EDADLRARVF DKIVAEHDRT IRMHRLITGQ DDLLADNAAL ARSVFNRFPY LEPLNHLQVE 

       910        920        930 
LLRRYRSGET DELVQRGILL TMSGLATALR NSG 

« Hide

References

[1]Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[2]"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700084 / mc(2)155.
[3]"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], IDENTIFICATION OF N-TERMINUS.
Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000480 Genomic DNA. Translation: ABK70062.1.
CP001663 Genomic DNA. Translation: AFP39483.1.
RefSeqYP_006567778.1. NC_018289.1.
YP_887412.1. NC_008596.1.

3D structure databases

ProteinModelPortalA0QWX4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246196.MSMEG_3097.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK70062; ABK70062; MSMEG_3097.
AFP39483; AFP39483; MSMEI_3019.
GeneID4535253.
KEGGmsg:MSMEI_3019.
msm:MSMEG_3097.
PATRIC18078620. VBIMycSme59918_3058.

Phylogenomic databases

eggNOGCOG2352.
HOGENOMHOG000238647.
KOK01595.
OMASILNCET.
OrthoDBEOG6TJ7T8.

Enzyme and pathway databases

BioCycMSME246196:GJ4Y-3097-MONOMER.

Family and domain databases

HAMAPMF_00595. PEPcase_type1.
InterProIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSPR00150. PEPCARBXLASE.
SUPFAMSSF51621. SSF51621. 1 hit.
PROSITEPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCAPP_MYCS2
AccessionPrimary (citable) accession number: A0QWX4
Secondary accession number(s): I7FL87
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families