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Protein

Phosphoenolpyruvate carboxylase

Gene

ppc

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.UniRule annotation

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei161 – 1611UniRule annotation
Active sitei593 – 5931UniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. phosphoenolpyruvate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbon fixation Source: UniProtKB-HAMAP
  2. oxaloacetate metabolic process Source: UniProtKB-HAMAP
  3. tricarboxylic acid cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbon dioxide fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3097-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylaseUniRule annotation (EC:4.1.1.31UniRule annotation)
Short name:
PEPCUniRule annotation
Short name:
PEPCaseUniRule annotation
Gene namesi
Name:ppcUniRule annotation
Ordered Locus Names:MSMEG_3097, MSMEI_3019
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Phosphoenolpyruvate carboxylasePRO_1000025566Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_3097.

Structurei

3D structure databases

ProteinModelPortaliA0QWX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 1 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2352.
HOGENOMiHOG000238647.
KOiK01595.
OMAiCGMGVIA.
OrthoDBiEOG6TJ7T8.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1.
InterProiIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 1 hit.
PROSITEiPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QWX4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADSNDTALE PFGSVQRTHI GREASEPMRE DIRLLGAILG DTVREQNGEE
60 70 80 90 100
VFDLVERARV ESFRVRRSEI DRSELADMFS GVDAHQAIPV IRAFTHFALL
110 120 130 140 150
ANVAEDIHRE RRRAVHVAAG KPPQDSSLAA TYRKLDAADL DVDKVADTLT
160 170 180 190 200
GALVSPVITA HPTETRRRTV FDTQHRITEL MRLRLHGHTR TDDNRDIETE
210 220 230 240 250
LRRHILTLWQ TALIRLSRLK ISDEIETGLR YYEAAFFDVI PQVNAEVRDA
260 270 280 290 300
LRKRWPDAKL LEEPILRPGS WIGGDRDGNP NVTPEVVRHA TGRAAYVALA
310 320 330 340 350
HYFEQITALE QELSMSARLV KVTPALAALA DACHEPARAD EPYRRALRVI
360 370 380 390 400
HARLTSTARE ILDEQPEHGL DLGLPRYQTP AEFLADLDAV DGSLRANGSR
410 420 430 440 450
VLADDRLGRL REAVRVFGFH LSGLDMRQNS DVHEEVVAEL LAWAGVHPDY
460 470 480 490 500
TSLSEPQRVE LLAAEIATRR PLIREGAELS ELAQKELGIV AAAARAVKVF
510 520 530 540 550
GPQAVPNYII SMCQSVSDML EAAVLLKEAG LLDISGSTPY APVGVVPLFE
560 570 580 590 600
TIDDLQRGSS ILEAALDLPE YRTMVDARDG HQEVMLGYSD SNKDGGYLAA
610 620 630 640 650
NWALYRAELD LVESARKTGI RLRLFHGRGG TVGRGGGPSY DAILAQPPGA
660 670 680 690 700
VKGSLRITEQ GEVIAAKYAE PRIAHRNLET LLAATLEASL LDVEGLGEEA
710 720 730 740 750
EPAYQVLDEL AALAQRAYSE LVHETPGFVE YFKTSTPVSE IGALNIGSRP
760 770 780 790 800
TSRKPTTSIA DLRAIPWVLA WSQSRVMLPG WYGTGSAFEN WIGTDPDGAR
810 820 830 840 850
LRVLQDLYAR WPFFRTVLSN MAQVLAKADM GLAARYSELV EDADLRARVF
860 870 880 890 900
DKIVAEHDRT IRMHRLITGQ DDLLADNAAL ARSVFNRFPY LEPLNHLQVE
910 920 930
LLRRYRSGET DELVQRGILL TMSGLATALR NSG
Length:933
Mass (Da):103,351
Last modified:January 9, 2007 - v1
Checksum:i33F2EAC46B98F7D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70062.1.
CP001663 Genomic DNA. Translation: AFP39483.1.
RefSeqiYP_006567778.1. NC_018289.1.
YP_887412.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70062; ABK70062; MSMEG_3097.
AFP39483; AFP39483; MSMEI_3019.
GeneIDi4535253.
KEGGimsm:MSMEG_3097.
PATRICi18078620. VBIMycSme59918_3058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70062.1.
CP001663 Genomic DNA. Translation: AFP39483.1.
RefSeqiYP_006567778.1. NC_018289.1.
YP_887412.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QWX4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK70062; ABK70062; MSMEG_3097.
AFP39483; AFP39483; MSMEI_3019.
GeneIDi4535253.
KEGGimsm:MSMEG_3097.
PATRICi18078620. VBIMycSme59918_3058.

Phylogenomic databases

eggNOGiCOG2352.
HOGENOMiHOG000238647.
KOiK01595.
OMAiCGMGVIA.
OrthoDBiEOG6TJ7T8.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3097-MONOMER.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1.
InterProiIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 1 hit.
PROSITEiPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], IDENTIFICATION OF N-TERMINUS.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiCAPP_MYCS2
AccessioniPrimary (citable) accession number: A0QWX4
Secondary accession number(s): I7FL87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: February 4, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.