A0QWX4 (CAPP_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase
Short name=PEPC
Short name=PEPCase
EC=4.1.1.31

Gene names

Name:	ppc
Ordered Locus Names:	MSMEG_3097, MSMEI_3019

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	933 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP-Rule MF_00595
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_00595
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the PEPCase type 1 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: HAMAP oxaloacetate metabolic process Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 933	933	Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00595		PRO_1000025566
Sites
	Active site	161	1	By similarity
	Active site	593	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QWX4 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 33F2EAC46B98F7D8	FASTA	933	103,351
	10 20 30 40 50 60 MADSNDTALE PFGSVQRTHI GREASEPMRE DIRLLGAILG DTVREQNGEE VFDLVERARV 70 80 90 100 110 120 ESFRVRRSEI DRSELADMFS GVDAHQAIPV IRAFTHFALL ANVAEDIHRE RRRAVHVAAG 130 140 150 160 170 180 KPPQDSSLAA TYRKLDAADL DVDKVADTLT GALVSPVITA HPTETRRRTV FDTQHRITEL 190 200 210 220 230 240 MRLRLHGHTR TDDNRDIETE LRRHILTLWQ TALIRLSRLK ISDEIETGLR YYEAAFFDVI 250 260 270 280 290 300 PQVNAEVRDA LRKRWPDAKL LEEPILRPGS WIGGDRDGNP NVTPEVVRHA TGRAAYVALA 310 320 330 340 350 360 HYFEQITALE QELSMSARLV KVTPALAALA DACHEPARAD EPYRRALRVI HARLTSTARE 370 380 390 400 410 420 ILDEQPEHGL DLGLPRYQTP AEFLADLDAV DGSLRANGSR VLADDRLGRL REAVRVFGFH 430 440 450 460 470 480 LSGLDMRQNS DVHEEVVAEL LAWAGVHPDY TSLSEPQRVE LLAAEIATRR PLIREGAELS 490 500 510 520 530 540 ELAQKELGIV AAAARAVKVF GPQAVPNYII SMCQSVSDML EAAVLLKEAG LLDISGSTPY 550 560 570 580 590 600 APVGVVPLFE TIDDLQRGSS ILEAALDLPE YRTMVDARDG HQEVMLGYSD SNKDGGYLAA 610 620 630 640 650 660 NWALYRAELD LVESARKTGI RLRLFHGRGG TVGRGGGPSY DAILAQPPGA VKGSLRITEQ 670 680 690 700 710 720 GEVIAAKYAE PRIAHRNLET LLAATLEASL LDVEGLGEEA EPAYQVLDEL AALAQRAYSE 730 740 750 760 770 780 LVHETPGFVE YFKTSTPVSE IGALNIGSRP TSRKPTTSIA DLRAIPWVLA WSQSRVMLPG 790 800 810 820 830 840 WYGTGSAFEN WIGTDPDGAR LRVLQDLYAR WPFFRTVLSN MAQVLAKADM GLAARYSELV 850 860 870 880 890 900 EDADLRARVF DKIVAEHDRT IRMHRLITGQ DDLLADNAAL ARSVFNRFPY LEPLNHLQVE 910 920 930 LLRRYRSGET DELVQRGILL TMSGLATALR NSG

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], IDENTIFICATION OF N-TERMINUS. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK70062.1. CP001663 Genomic DNA. Translation: AFP39483.1.
RefSeq	YP_006567778.1. NC_018289.1. YP_887412.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QWX4.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_3097.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK70062; ABK70062; MSMEG_3097.
GeneID	13425386. 4535253.
KEGG	msg:MSMEI_3019. msm:MSMEG_3097.
PATRIC	18078620. VBIMycSme59918_3058.
Phylogenomic databases
eggNOG	COG2352.
HOGENOM	HOG000238647.
KO	K01595.
OMA	AIPWVFG.
ProtClustDB	PRK00009.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-3097-MONOMER.
Family and domain databases
HAMAP	MF_00595. PEPcase_type1.
InterPro	IPR021135. PEP_COase. IPR018129. PEP_COase_AS. IPR022805. PEP_COase_bac/pln-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP_MYCS2

Accession

Primary (citable) accession number: A0QWX4
Secondary accession number(s): I7FL87

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program