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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk), Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40SubstrateUniRule annotation1
Binding sitei122SubstrateUniRule annotation1
Binding sitei166SubstrateUniRule annotation1
Binding sitei216ATPUniRule annotation1
Binding sitei304ATP; via carbonyl oxygenUniRule annotation1
Binding sitei335ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi364 – 367ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinaseUniRule annotation (EC:2.7.2.3UniRule annotation)
Gene namesi
Name:pgkUniRule annotation
Ordered Locus Names:MSMEG_3085, MSMEI_3007
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_10000096312 – 408Phosphoglycerate kinaseAdd BLAST407

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_3085.

Structurei

3D structure databases

ProteinModelPortaliA0QWW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 26Substrate bindingUniRule annotation3
Regioni63 – 66Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105BZA. Bacteria.
COG0126. LUCA.
HOGENOMiHOG000227107.
KOiK00927.
OMAiAGHPVGK.
OrthoDBiPOG091H00YN.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0QWW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVKTLDDLL AEGVQGRGVL VRSDLNVPLD DDGNITDPGR VIASVPTLQA
60 70 80 90 100
LAEAGAKVIV TAHLGRPKGE PDPKLSLAPV AAALGEKLGR HVQLAGDVVG
110 120 130 140 150
TDALARAEGL TDGDVLLLEN IRFDARETSK DDSERLSLAK ALAALVEGPD
160 170 180 190 200
GSPGVFVSDG FGVVHRKQAS VYDVATLLPH YAGTLVAAEV KVLQQLTSST
210 220 230 240 250
DRPYAVVLGG SKVSDKLAVI ENLATKADSL IIGGGMCFTF LAAQGFSVGS
260 270 280 290 300
SLLQEEMVDT CRRLLDEYAD VIHLPVDIVV ADKFAADAEA ETVAADRIPD
310 320 330 340 350
GKMGLDIGPG SVERFTALLS NAKTVFWNGP MGVFEFPAFA AGTKGVAEAI
360 370 380 390 400
IGATGKGAFS VVGGGDSAAA VRRLGLPEDG FSHISTGGGA SLEYLEGKEL

PGIQVLES
Length:408
Mass (Da):42,095
Last modified:January 9, 2007 - v1
Checksum:i30FC1D53C4AEEB96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71882.1.
CP001663 Genomic DNA. Translation: AFP39471.1.
RefSeqiWP_003894473.1. NZ_CP009494.1.
YP_887401.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK71882; ABK71882; MSMEG_3085.
AFP39471; AFP39471; MSMEI_3007.
GeneIDi4537204.
KEGGimsb:LJ00_15350.
msg:MSMEI_3007.
msm:MSMEG_3085.
PATRICi18078596. VBIMycSme59918_3046.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK71882.1.
CP001663 Genomic DNA. Translation: AFP39471.1.
RefSeqiWP_003894473.1. NZ_CP009494.1.
YP_887401.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QWW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3085.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK71882; ABK71882; MSMEG_3085.
AFP39471; AFP39471; MSMEI_3007.
GeneIDi4537204.
KEGGimsb:LJ00_15350.
msg:MSMEI_3007.
msm:MSMEG_3085.
PATRICi18078596. VBIMycSme59918_3046.

Phylogenomic databases

eggNOGiENOG4105BZA. Bacteria.
COG0126. LUCA.
HOGENOMiHOG000227107.
KOiK00927.
OMAiAGHPVGK.
OrthoDBiPOG091H00YN.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK_MYCS2
AccessioniPrimary (citable) accession number: A0QWW3
Secondary accession number(s): I7G8D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.