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Protein

Methionyl-tRNA formyltransferase

Gene

fmt

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP.UniRule annotation

Catalytic activityi

10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet).UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3064-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionyl-tRNA formyltransferaseUniRule annotation (EC:2.1.2.9UniRule annotation)
Gene namesi
Name:fmtUniRule annotation
Ordered Locus Names:MSMEG_3064, MSMEI_2988
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Methionyl-tRNA formyltransferasePRO_1000020101Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_3064.

Structurei

3D structure databases

ProteinModelPortaliA0QWU2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni110 – 1134Tetrahydrofolate (THF) bindingUniRule annotation

Sequence similaritiesi

Belongs to the Fmt family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CAE. Bacteria.
COG0223. LUCA.
HOGENOMiHOG000261177.
KOiK00604.
OMAiGCINSHA.
OrthoDBiPOG091H01YM.

Family and domain databases

Gene3Di3.10.25.10. 1 hit.
3.40.50.170. 1 hit.
HAMAPiMF_00182. Formyl_trans. 1 hit.
InterProiIPR005794. Fmt.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR011034. Formyl_transferase_C-like.
[Graphical view]
PfamiPF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
SSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00460. fmt. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QWU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLVFAGTPE PALPSLRRLI ESPRHDVVAV LTRPDAAAGR RGKPRPSPVA
60 70 80 90 100
QLALEHGIPL LRPDRPNSDE FVAELTELAP DCCAVVAYGA LLSQRLLAVP
110 120 130 140 150
RHGWINLHFS LLPAWRGAAP VQAAIAAGDT VTGATTFQIE PALDSGPVYG
160 170 180 190 200
VVTETVRDTD TAGDLLERLS DSGAELLERT IDGIADGSLT AVPQPSEGIT
210 220 230 240 250
VAPKITVESA RVRWDLPAHV VDRRIRAVTP NPGAWTMIGE LRVKVGPVTV
260 270 280 290 300
DQAAEADGPL APGEIRVGRN SVHVGTGSHP VRLGQIQPPG KKLMNAADWA
310
RGARLEEPVS AS
Length:312
Mass (Da):33,008
Last modified:January 9, 2007 - v1
Checksum:iF179910503BA9F6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70808.1.
CP001663 Genomic DNA. Translation: AFP39452.1.
RefSeqiWP_011728792.1. NZ_CP009494.1.
YP_887380.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70808; ABK70808; MSMEG_3064.
AFP39452; AFP39452; MSMEI_2988.
GeneIDi4535023.
KEGGimsb:LJ00_15245.
msg:MSMEI_2988.
msm:MSMEG_3064.
PATRICi18078554. VBIMycSme59918_3025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70808.1.
CP001663 Genomic DNA. Translation: AFP39452.1.
RefSeqiWP_011728792.1. NZ_CP009494.1.
YP_887380.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QWU2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK70808; ABK70808; MSMEG_3064.
AFP39452; AFP39452; MSMEI_2988.
GeneIDi4535023.
KEGGimsb:LJ00_15245.
msg:MSMEI_2988.
msm:MSMEG_3064.
PATRICi18078554. VBIMycSme59918_3025.

Phylogenomic databases

eggNOGiENOG4105CAE. Bacteria.
COG0223. LUCA.
HOGENOMiHOG000261177.
KOiK00604.
OMAiGCINSHA.
OrthoDBiPOG091H01YM.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3064-MONOMER.

Family and domain databases

Gene3Di3.10.25.10. 1 hit.
3.40.50.170. 1 hit.
HAMAPiMF_00182. Formyl_trans. 1 hit.
InterProiIPR005794. Fmt.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR011034. Formyl_transferase_C-like.
[Graphical view]
PfamiPF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
SSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00460. fmt. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFMT_MYCS2
AccessioniPrimary (citable) accession number: A0QWU2
Secondary accession number(s): I7G177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.