A0QWS1 (PYRB_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	MSMEG_3043

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000301591

Sequences

	Sequence		Length	Mass (Da)
	A0QWS1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 32345976A94125D8	FASTA	318	34,349
	10 20 30 40 50 60 MTKRHLLSAG DLTRDDATAI LDDADRFREA LLGREVKKLP TLRGRTIITM FYENSTRTRV 70 80 90 100 110 120 SFEVAGKWMS ADVINVSASG SSVAKGESLR DTALTLRAAG ADALIIRHPA SGAAQQLAEW 130 140 150 160 170 180 TVEEDGGGPS VINAGDGTHE HPTQALLDAL TIRQRLGSVE GKRVVIVGDV LHSRVARSNV 190 200 210 220 230 240 TLLHTLGAEV VLVSPPTLLP RGVENWPVTV SYDLDAELPA ADAVLMLRVQ AERMNGSFFP 250 260 270 280 290 300 SAREYSVRYG LSEKRQAMLP DRAIVLHPGP MVRGMEISFP VADSPQSAVL QQVSNGVHVR 310 MAVLFHLLVG ADREAINV

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK74784.1.
RefSeq	YP_887359.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QWS1.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QWS1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000045229; EBMYCP00000043566; EBMYCG00000045224.
GeneID	4536879.
GenomeReviews	Gene locus MSMEG_3043 in contig CP000480_GR.
KEGG	msm:MSMEG_3043.
NMPDR	fig|246196.1.peg.3057.
PATRIC	18078514. VBIMycSme59918_3005.
TIGR	MSMEG_3043.
Phylogenomic databases
eggNOG	COG0540.
GeneTree	EBGT00050000016463.
HOGENOM	HBG579429.
OMA	LQRERMS.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_3043-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_MYCS2

Accession

Primary (citable) accession number: A0QWS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program