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Protein

Chorismate synthase

Gene

aroC

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotationNote: Reduced FMN (FMNH(2)).UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (MSMEG_2799), Phospho-2-dehydro-3-deoxyheptonate aldolase AroG (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase (MSMEI_2729)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ)
  4. no protein annotated in this organism
  5. Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40NADPUniRule annotation1
Binding sitei46NADPUniRule annotation1
Binding sitei299FMN; via amide nitrogenUniRule annotation1
Binding sitei340FMNUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 136FMNUniRule annotation3
Nucleotide bindingi255 – 256FMNUniRule annotation2
Nucleotide bindingi314 – 318FMNUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandFAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
Ordered Locus Names:MSMEG_3030, MSMEI_2955
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003224101 – 399Chorismate synthaseAdd BLAST399

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_3030

Structurei

3D structure databases

ProteinModelPortaliA0QWQ9
SMRiA0QWQ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10 Bacteria
COG0082 LUCA
HOGENOMiHOG000060334
KOiK01736
OMAiMLSINAV
OrthoDBiPOG091H00EG

Family and domain databases

CDDicd07304 Chorismate_synthase, 1 hit
Gene3Di3.60.150.10, 1 hit
HAMAPiMF_00300 Chorismate_synth, 1 hit
InterProiView protein in InterPro
IPR000453 Chorismate_synth
IPR035904 Chorismate_synth_AroC_sf
IPR020541 Chorismate_synthase_CS
PANTHERiPTHR21085 PTHR21085, 1 hit
PfamiView protein in Pfam
PF01264 Chorismate_synt, 1 hit
PIRSFiPIRSF001456 Chorismate_synth, 1 hit
SUPFAMiSSF103263 SSF103263, 1 hit
TIGRFAMsiTIGR00033 aroC, 1 hit
PROSITEiView protein in PROSITE
PS00787 CHORISMATE_SYNTHASE_1, 1 hit
PS00788 CHORISMATE_SYNTHASE_2, 1 hit
PS00789 CHORISMATE_SYNTHASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

A0QWQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRWTTAGES HGRALVAMLE GMVAGVPITS EEIGAQLKRR RLGYGRGARM
60 70 80 90 100
KFEQDQVTML AGVRHGLTLG GPIAIEIGNT EWPKWESVMS PDPVDPADLD
110 120 130 140 150
VARNAPLTRP RPGHADYAGM LKYGFDDARP VLERASARET AARVAAGTVA
160 170 180 190 200
RAFLREALGV EVLSHVISIG ASKPYDGPAP QFSDLSAIDD SPVRAFDKAS
210 220 230 240 250
EELMIAEIEA AKRDGDTLGG VVEVVADGLP VGLGSFTSGE NRLDSQLAAA
260 270 280 290 300
VMGIQAIKGV EIGDGFETAR RRGSVAHDEM YPGPDGVVRS TNRAGGLEGG
310 320 330 340 350
MTNGQPLRVR AAMKPISTVP RALATVDMTS GEEAVAIHQR SDVCAVPAAG
360 370 380 390
VVVETMVALV LARAALEKFG GDSLAETRAN IDSYLRAVAE REPAAQASS
Length:399
Mass (Da):41,944
Last modified:January 9, 2007 - v1
Checksum:i824E9AC671C6FF3A
GO

Sequence cautioni

The sequence AFP39419 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK72490.1
CP001663 Genomic DNA Translation: AFP39419.1 Different initiation.
RefSeqiWP_003894412.1, NZ_CP009494.1
YP_887347.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK72490; ABK72490; MSMEG_3030
AFP39419; AFP39419; MSMEI_2955
GeneIDi4533365
KEGGimsb:LJ00_15080
msg:MSMEI_2955
msm:MSMEG_3030
PATRICifig|246196.19.peg.2992

Similar proteinsi

Entry informationi

Entry nameiAROC_MYCS2
AccessioniPrimary (citable) accession number: A0QWQ9
Secondary accession number(s): I7GAB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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