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Protein

Chorismate synthase

Gene

aroC

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotationNote: Reduced FMN (FMNH(2)).UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (MSMEG_2799), Phospho-2-dehydro-3-deoxyheptonate aldolase AroG (aroG)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. no protein annotated in this organism
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401NADPUniRule annotation
Binding sitei46 – 461NADPUniRule annotation
Binding sitei299 – 2991FMN; via amide nitrogenUniRule annotation
Binding sitei340 – 3401FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 1363FMNUniRule annotation
Nucleotide bindingi255 – 2562FMNUniRule annotation
Nucleotide bindingi314 – 3185FMNUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-3030-MONOMER.
UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
Ordered Locus Names:MSMEG_3030, MSMEI_2955
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Chorismate synthasePRO_0000322410Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_3030.

Structurei

3D structure databases

ProteinModelPortaliA0QWQ9.
SMRiA0QWQ9. Positions 1-391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
KOiK01736.
OMAiMLSINAV.
OrthoDBiPOG091H00EG.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 2 hits.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QWQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRWTTAGES HGRALVAMLE GMVAGVPITS EEIGAQLKRR RLGYGRGARM
60 70 80 90 100
KFEQDQVTML AGVRHGLTLG GPIAIEIGNT EWPKWESVMS PDPVDPADLD
110 120 130 140 150
VARNAPLTRP RPGHADYAGM LKYGFDDARP VLERASARET AARVAAGTVA
160 170 180 190 200
RAFLREALGV EVLSHVISIG ASKPYDGPAP QFSDLSAIDD SPVRAFDKAS
210 220 230 240 250
EELMIAEIEA AKRDGDTLGG VVEVVADGLP VGLGSFTSGE NRLDSQLAAA
260 270 280 290 300
VMGIQAIKGV EIGDGFETAR RRGSVAHDEM YPGPDGVVRS TNRAGGLEGG
310 320 330 340 350
MTNGQPLRVR AAMKPISTVP RALATVDMTS GEEAVAIHQR SDVCAVPAAG
360 370 380 390
VVVETMVALV LARAALEKFG GDSLAETRAN IDSYLRAVAE REPAAQASS
Length:399
Mass (Da):41,944
Last modified:January 9, 2007 - v1
Checksum:i824E9AC671C6FF3A
GO

Sequence cautioni

The sequence AFP39419 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72490.1.
CP001663 Genomic DNA. Translation: AFP39419.1. Different initiation.
RefSeqiWP_003894412.1. NZ_CP009494.1.
YP_887347.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK72490; ABK72490; MSMEG_3030.
AFP39419; AFP39419; MSMEI_2955.
GeneIDi4533365.
KEGGimsb:LJ00_15080.
msg:MSMEI_2955.
msm:MSMEG_3030.
PATRICi18078488. VBIMycSme59918_2992.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72490.1.
CP001663 Genomic DNA. Translation: AFP39419.1. Different initiation.
RefSeqiWP_003894412.1. NZ_CP009494.1.
YP_887347.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QWQ9.
SMRiA0QWQ9. Positions 1-391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_3030.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK72490; ABK72490; MSMEG_3030.
AFP39419; AFP39419; MSMEI_2955.
GeneIDi4533365.
KEGGimsb:LJ00_15080.
msg:MSMEI_2955.
msm:MSMEG_3030.
PATRICi18078488. VBIMycSme59918_2992.

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060334.
KOiK01736.
OMAiMLSINAV.
OrthoDBiPOG091H00EG.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.
BioCyciMSME246196:GJ4Y-3030-MONOMER.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 2 hits.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROC_MYCS2
AccessioniPrimary (citable) accession number: A0QWQ9
Secondary accession number(s): I7GAB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.