A0QWJ5 (APT_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenine phosphoribosyltransferase
Short name=APRT
EC=2.4.2.7

Gene names

Name:	apt
Ordered Locus Names:	MSMEG_2964

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis By similarity. HAMAP MF_00004
Catalytic activity	AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00004
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1. HAMAP MF_00004
Subunit structure	Homodimer By similarity. HAMAP MF_00004
Subcellular location	Cytoplasm By similarity HAMAP MF_00004.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	adenine salvage Inferred from electronic annotation. Source: InterPro purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenine phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	Adenine phosphoribosyltransferase HAMAP MF_00004		PRO_1000000311

Sequences

	Sequence		Length	Mass (Da)
	A0QWJ5 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 2344344598FB071D	FASTA	180	18,385
	10 20 30 40 50 60 MTQHHDTAEV SRVIATLTRE VADFPEPGIQ FKDLTPLLAD ARGLRVVTDA LADIASGADL 70 80 90 100 110 120 VAGLDARGFL LGAAVATRLG TGVLAVRKGG KLPPPVHGAT YQLEYGTATL EIPAEGIDIA 130 140 150 160 170 180 GRNVVIIDDV LATGGTLAAA ARLLGDCGAN VTGAGVVLEL EALRGREAVA PLGVRSLHII

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed: 18955433] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK71166.1.
RefSeq	YP_887283.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QWJ5.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QWJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000041325; EBMYCP00000039662; EBMYCG00000041320.
GeneID	4535093.
GenomeReviews	Gene locus MSMEG_2964 in contig CP000480_GR.
KEGG	msm:MSMEG_2964.
NMPDR	fig|246196.1.peg.2976.
PATRIC	18078358. VBIMycSme59918_2927.
TIGR	MSMEG_2964.
Phylogenomic databases
eggNOG	COG0503.
GeneTree	EBGT00050000016328.
HOGENOM	HBG703830.
OMA	GMEARGF.
ProtClustDB	PRK02304.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_2964-MONOMER.
Family and domain databases
HAMAP	MF_00004. Aden_phosphoribosyltr. [Tree]
InterPro	IPR005764. Ade_phspho_trans. IPR000836. PRibTrfase. [Graphical view]
KO	K00759.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APT_MYCS2

Accession

Primary (citable) accession number: A0QWJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program