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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Metal bindingi68MagnesiumUniRule annotation1
Metal bindingi141MagnesiumUniRule annotation1
Metal bindingi144MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:MSMEG_2943, MSMEI_2869
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000027791 – 185Crossover junction endodeoxyribonuclease RuvCAdd BLAST185

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_2943

Structurei

3D structure databases

ProteinModelPortaliA0QWH4
SMRiA0QWH4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU Bacteria
COG0817 LUCA
HOGENOMiHOG000012182
KOiK01159
OMAiGLTRCGI
OrthoDBiPOG091H0202

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR020563 X-over_junc_endoDNase_Mg_BS
IPR002176 X-over_junc_endoDNase_RuvC
PANTHERiPTHR30194 PTHR30194, 1 hit
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit
PROSITEiView protein in PROSITE
PS01321 RUVC, 1 hit

Sequencei

Sequence statusi: Complete.

A0QWH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVMGVDPGL TRCGLSMIES GKGRQVIALD VDVVRTPADT PLQKRLLTIS
60 70 80 90 100
DAAEHWMDTH RPDVIAIERV FANQNANTAM GTAQAGGVIA LAAAKRDIEV
110 120 130 140 150
HFHTPSEVKA AVTGSGRADK SQVTEMVTRI LALQAKPTPA DAADALALAI
160 170 180
CHCWRAPMIA RMAAAEAMAE EQRRKFQAKI KAARG
Length:185
Mass (Da):19,805
Last modified:January 9, 2007 - v1
Checksum:i5438C9D8C4BF7B6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK76031.1
CP001663 Genomic DNA Translation: AFP39333.1
RefSeqiWP_003894321.1, NZ_CP009494.1
YP_887262.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK76031; ABK76031; MSMEG_2943
AFP39333; AFP39333; MSMEI_2869
GeneIDi4533548
KEGGimsb:LJ00_14645
msg:MSMEI_2869
msm:MSMEG_2943
PATRICifig|246196.19.peg.2906

Entry informationi

Entry nameiRUVC_MYCS2
AccessioniPrimary (citable) accession number: A0QWH4
Secondary accession number(s): I7FKR3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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