A0QW23 (DCUP_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uroporphyrinogen decarboxylase
Short name=UPD
Short name=URO-D
EC=4.1.1.37

Gene names

Name:	hemE
Ordered Locus Names:	MSMEG_2780

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III By similarity. HAMAP MF_00218
Catalytic activity	Uroporphyrinogen III = coproporphyrinogen + 4 CO2. HAMAP MF_00218
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4. HAMAP MF_00218
Subunit structure	Homodimer By similarity. HAMAP MF_00218
Subcellular location	Cytoplasm By similarity HAMAP MF_00218.
Sequence similarities	Belongs to the uroporphyrinogen decarboxylase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	uroporphyrinogen decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Uroporphyrinogen decarboxylase HAMAP MF_00218		PRO_1000023921
Regions
	Region	30 – 34	5	Substrate binding By similarity
Sites
	Binding site	79	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	209	1	Substrate By similarity
	Binding site	332	1	Substrate By similarity
	Site	79	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QW23 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: BC4FAA02D6C9F803	FASTA	353	37,410
	10 20 30 40 50 60 MNTRRELPES PYLAAASGRS PHRVPVWFMR QAGRSLPEYR ELRAQHRMLQ ACFDAELVCE 70 80 90 100 110 120 ITMQPVRRHK VDAAILFSDI VVPLKAAGIG LDIVPDVGPV IDNPIRTLGD VQAMPALESP 130 140 150 160 170 180 QVAPVAEAVR LLTAELGDVP LIGFAGAPFT LASYLVEGGP SRHHERTKAM MLGESSTWHA 190 200 210 220 230 240 LMTALTDLTI AFLQAQVDAG VDALQVFDSW AGTLSLTDYR EYVLPHSSRV FATMAAAGVP 250 260 270 280 290 300 MTHFGVGTAE LLGAMSEALA PGAARVVGVD WRTSLADAAA RVLPGAALQG NLDPVVLLAG 310 320 330 340 350 WPVVEKAVRR VVEDGRAAVA AGAAGHIFNL GHGVLPATDP GIITDAVELV HSL

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed: 18955433] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK69905.1.
RefSeq	YP_887111.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QW23.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QW23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000045151; EBMYCP00000043488; EBMYCG00000045146.
GeneID	4534005.
GenomeReviews	Gene locus MSMEG_2780 in contig CP000480_GR.
KEGG	msm:MSMEG_2780.
NMPDR	fig|246196.1.peg.2780.
PATRIC	18077992. VBIMycSme59918_2748.
TIGR	MSMEG_2780.
Phylogenomic databases
eggNOG	COG0407.
GeneTree	EBGT00050000016469.
HOGENOM	HBG628392.
OMA	PRIHFGV.
PhylomeDB	A0QW23.
ProtClustDB	PRK00115.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_2780-MONOMER.
Family and domain databases
HAMAP	MF_00218. URO-D. [Tree]
InterPro	IPR006361. Uroporphyrinogen_deCO2ase_HemE. IPR000257. Uroporphyrinogen_deCOase. [Graphical view]
KO	K01599.
PANTHER	PTHR21091:SF2. HemE. 1 hit.
Pfam	PF01208. URO-D. 1 hit. [Graphical view]
TIGRFAMs	TIGR01464. HemE. 1 hit.
PROSITE	PS00906. UROD_1. 1 hit. PS00907. UROD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCUP_MYCS2

Accession

Primary (citable) accession number: A0QW23

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program