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Protein

Uroporphyrinogen decarboxylase

Gene

hemE

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.UniRule annotation

Catalytic activityi

Uroporphyrinogen III = coproporphyrinogen + 4 CO2.UniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Porphobilinogen deaminase (hemC), Porphobilinogen deaminase (hemC)
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (hemE)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateUniRule annotation
Sitei79 – 791Transition state stabilizerUniRule annotation
Binding sitei154 – 1541SubstrateUniRule annotation
Binding sitei209 – 2091SubstrateUniRule annotation
Binding sitei332 – 3321SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2780-MONOMER.
UniPathwayiUPA00251; UER00321.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen decarboxylaseUniRule annotation (EC:4.1.1.37UniRule annotation)
Short name:
UPDUniRule annotation
Short name:
URO-DUniRule annotation
Gene namesi
Name:hemEUniRule annotation
Ordered Locus Names:MSMEG_2780, MSMEI_2711
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Uroporphyrinogen decarboxylasePRO_1000023921Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_2780.

Structurei

3D structure databases

ProteinModelPortaliA0QW23.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 345Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the uroporphyrinogen decarboxylase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CFZ. Bacteria.
COG0407. LUCA.
HOGENOMiHOG000253897.
KOiK01599.
OMAiGSSKDFR.
OrthoDBiPOG091H040E.

Family and domain databases

CDDicd00717. URO-D. 1 hit.
HAMAPiMF_00218. URO_D. 1 hit.
InterProiIPR006361. Uroporphyrinogen_deCO2ase_HemE.
IPR000257. Uroporphyrinogen_deCOase.
[Graphical view]
PfamiPF01208. URO-D. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01464. hemE. 1 hit.
PROSITEiPS00906. UROD_1. 1 hit.
PS00907. UROD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QW23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTRRELPES PYLAAASGRS PHRVPVWFMR QAGRSLPEYR ELRAQHRMLQ
60 70 80 90 100
ACFDAELVCE ITMQPVRRHK VDAAILFSDI VVPLKAAGIG LDIVPDVGPV
110 120 130 140 150
IDNPIRTLGD VQAMPALESP QVAPVAEAVR LLTAELGDVP LIGFAGAPFT
160 170 180 190 200
LASYLVEGGP SRHHERTKAM MLGESSTWHA LMTALTDLTI AFLQAQVDAG
210 220 230 240 250
VDALQVFDSW AGTLSLTDYR EYVLPHSSRV FATMAAAGVP MTHFGVGTAE
260 270 280 290 300
LLGAMSEALA PGAARVVGVD WRTSLADAAA RVLPGAALQG NLDPVVLLAG
310 320 330 340 350
WPVVEKAVRR VVEDGRAAVA AGAAGHIFNL GHGVLPATDP GIITDAVELV

HSL
Length:353
Mass (Da):37,410
Last modified:January 9, 2007 - v1
Checksum:iBC4FAA02D6C9F803
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69905.1.
CP001663 Genomic DNA. Translation: AFP39179.1.
RefSeqiWP_011728579.1. NZ_CP009494.1.
YP_887111.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK69905; ABK69905; MSMEG_2780.
AFP39179; AFP39179; MSMEI_2711.
GeneIDi4534005.
KEGGimsb:LJ00_13820.
msg:MSMEI_2711.
msm:MSMEG_2780.
PATRICi18077992. VBIMycSme59918_2748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK69905.1.
CP001663 Genomic DNA. Translation: AFP39179.1.
RefSeqiWP_011728579.1. NZ_CP009494.1.
YP_887111.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QW23.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_2780.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK69905; ABK69905; MSMEG_2780.
AFP39179; AFP39179; MSMEI_2711.
GeneIDi4534005.
KEGGimsb:LJ00_13820.
msg:MSMEI_2711.
msm:MSMEG_2780.
PATRICi18077992. VBIMycSme59918_2748.

Phylogenomic databases

eggNOGiENOG4105CFZ. Bacteria.
COG0407. LUCA.
HOGENOMiHOG000253897.
KOiK01599.
OMAiGSSKDFR.
OrthoDBiPOG091H040E.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00321.
BioCyciMSME246196:GJ4Y-2780-MONOMER.

Family and domain databases

CDDicd00717. URO-D. 1 hit.
HAMAPiMF_00218. URO_D. 1 hit.
InterProiIPR006361. Uroporphyrinogen_deCO2ase_HemE.
IPR000257. Uroporphyrinogen_deCOase.
[Graphical view]
PfamiPF01208. URO-D. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01464. hemE. 1 hit.
PROSITEiPS00906. UROD_1. 1 hit.
PS00907. UROD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCUP_MYCS2
AccessioniPrimary (citable) accession number: A0QW23
Secondary accession number(s): I7G9G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.