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Protein

LexA repressor

Gene

lexA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.UniRule annotation

Catalytic activityi

Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei167For autocatalytic cleavage activityUniRule annotation1
Active sitei204For autocatalytic cleavage activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi56 – 76H-T-H motifUniRule annotationAdd BLAST21

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Hydrolase, Repressor
Biological processDNA damage, DNA repair, DNA replication, SOS response, Transcription, Transcription regulation

Protein family/group databases

MEROPSiS24.001

Names & Taxonomyi

Protein namesi
Recommended name:
LexA repressorUniRule annotation (EC:3.4.21.88UniRule annotation)
Gene namesi
Name:lexAUniRule annotation
Ordered Locus Names:MSMEG_2740, MSMEI_2673
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  • protein-DNA complex Source: CollecTF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003227451 – 243LexA repressorAdd BLAST243

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei132 – 133Cleavage; by autolysisUniRule annotation2

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PRIDEiA0QVY5

Expressioni

Gene expression databases

CollecTFiEXPREG_00000170

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_2740

Structurei

3D structure databases

ProteinModelPortaliA0QVY5
SMRiA0QVY5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S24 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DS7 Bacteria
COG1974 LUCA
HOGENOMiHOG000232168
KOiK01356
OMAiRQMMQAM
OrthoDBiPOG091H029U

Family and domain databases

Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00015 LexA, 1 hit
InterProiView protein in InterPro
IPR006200 LexA
IPR036286 LexA/Signal_pep-like_sf
IPR006199 LexA_DNA-bd_dom
IPR006197 Peptidase_S24_LexA
IPR015927 Peptidase_S24_S26A/B/C
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF01726 LexA_DNA_bind, 1 hit
PF00717 Peptidase_S24, 1 hit
PRINTSiPR00726 LEXASERPTASE
SUPFAMiSSF46785 SSF46785, 1 hit
SSF51306 SSF51306, 1 hit
TIGRFAMsiTIGR00498 lexA, 1 hit

Sequencei

Sequence statusi: Complete.

A0QVY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDTGEFTD GSTESPADAD GAGRRRAVDN GLTERQRTIL EVIRASVTSR
60 70 80 90 100
GYPPSIREIG DAVGLTSTSS VAHQLRTLER KGYLRRDPNR PRAVDVRAAD
110 120 130 140 150
DPAAAAVVTT DVAGSDALPE PTFVPVLGRI AAGGPILAEE AVEDVFPLPR
160 170 180 190 200
ELVGEGSLFL LKVVGDSMVD AAICDGDWVV VRQQNVADNG DIVAAMIDGE
210 220 230 240
ATVKTFKRAR GQVWLMPHNP AYDPIPGNEA AVLGKVVTVI RKI
Length:243
Mass (Da):25,778
Last modified:January 9, 2007 - v1
Checksum:iA9831462CD0D503A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK70187.1
CP001663 Genomic DNA Translation: AFP39141.1
RefSeqiWP_003894124.1, NZ_CP009494.1
YP_887073.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK70187; ABK70187; MSMEG_2740
AFP39141; AFP39141; MSMEI_2673
GeneIDi4531324
KEGGimsb:LJ00_13625
msg:MSMEI_2673
msm:MSMEG_2740
PATRICifig|246196.19.peg.2708

Similar proteinsi

Entry informationi

Entry nameiLEXA_MYCS2
AccessioniPrimary (citable) accession number: A0QVY5
Secondary accession number(s): I7G7A4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health