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Protein

LexA repressor

Gene

lexA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.UniRule annotation

Catalytic activityi

Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei167 – 1671For autocatalytic cleavage activityUniRule annotation
Active sitei204 – 2041For autocatalytic cleavage activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi56 – 7621H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Repressor

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, SOS response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2740-MONOMER.

Protein family/group databases

MEROPSiS24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
LexA repressorUniRule annotation (EC:3.4.21.88UniRule annotation)
Gene namesi
Name:lexAUniRule annotation
Ordered Locus Names:MSMEG_2740, MSMEI_2673
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243LexA repressorPRO_0000322745Add
BLAST

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei132 – 1332Cleavage; by autolysisUniRule annotation

Keywords - PTMi

Autocatalytic cleavage

Expressioni

Gene expression databases

CollecTFiEXPREG_00000170.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_2740.

Structurei

3D structure databases

ProteinModelPortaliA0QVY5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S24 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DS7. Bacteria.
COG1974. LUCA.
HOGENOMiHOG000232168.
KOiK01356.
OMAiKQHELLM.
OrthoDBiPOG091H029U.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPiMF_00015. LexA. 1 hit.
InterProiIPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QVY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDTGEFTD GSTESPADAD GAGRRRAVDN GLTERQRTIL EVIRASVTSR
60 70 80 90 100
GYPPSIREIG DAVGLTSTSS VAHQLRTLER KGYLRRDPNR PRAVDVRAAD
110 120 130 140 150
DPAAAAVVTT DVAGSDALPE PTFVPVLGRI AAGGPILAEE AVEDVFPLPR
160 170 180 190 200
ELVGEGSLFL LKVVGDSMVD AAICDGDWVV VRQQNVADNG DIVAAMIDGE
210 220 230 240
ATVKTFKRAR GQVWLMPHNP AYDPIPGNEA AVLGKVVTVI RKI
Length:243
Mass (Da):25,778
Last modified:January 9, 2007 - v1
Checksum:iA9831462CD0D503A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70187.1.
CP001663 Genomic DNA. Translation: AFP39141.1.
RefSeqiWP_003894124.1. NZ_CP009494.1.
YP_887073.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70187; ABK70187; MSMEG_2740.
AFP39141; AFP39141; MSMEI_2673.
GeneIDi4531324.
KEGGimsb:LJ00_13625.
msg:MSMEI_2673.
msm:MSMEG_2740.
PATRICi18077912. VBIMycSme59918_2708.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70187.1.
CP001663 Genomic DNA. Translation: AFP39141.1.
RefSeqiWP_003894124.1. NZ_CP009494.1.
YP_887073.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QVY5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_2740.

Protein family/group databases

MEROPSiS24.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK70187; ABK70187; MSMEG_2740.
AFP39141; AFP39141; MSMEI_2673.
GeneIDi4531324.
KEGGimsb:LJ00_13625.
msg:MSMEI_2673.
msm:MSMEG_2740.
PATRICi18077912. VBIMycSme59918_2708.

Phylogenomic databases

eggNOGiENOG4105DS7. Bacteria.
COG1974. LUCA.
HOGENOMiHOG000232168.
KOiK01356.
OMAiKQHELLM.
OrthoDBiPOG091H029U.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2740-MONOMER.

Gene expression databases

CollecTFiEXPREG_00000170.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPiMF_00015. LexA. 1 hit.
InterProiIPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLEXA_MYCS2
AccessioniPrimary (citable) accession number: A0QVY5
Secondary accession number(s): I7G7A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.