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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N6-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 16ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:MSMEG_2734, MSMEI_2667
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000206211 – 302tRNA dimethylallyltransferaseAdd BLAST302

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei100Interaction with substrate tRNAUniRule annotation1
Sitei121Interaction with substrate tRNAUniRule annotation1

Protein-protein interaction databases

STRINGi246196.MSMEG_2734

Structurei

3D structure databases

ProteinModelPortaliA0QVX9
SMRiA0QVX9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 16Substrate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX Bacteria
COG0324 LUCA
HOGENOMiHOG000039995
KOiK00791
OMAiGTGLYIR
OrthoDBiPOG091H00NK

Family and domain databases

HAMAPiMF_00185 IPP_trans, 1 hit
InterProiView protein in InterPro
IPR018022 IPT
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01715 IPPT, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00174 miaA, 1 hit

Sequencei

Sequence statusi: Complete.

A0QVX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRPLAVIGP TGTGKSALAL EFAERVGGEI VNADAMQQYR GMDIGTAKLT
60 70 80 90 100
VEERRGIPHH QLDVLDVVET ATVARYQQAA AADIETIAAR GATPVIVGGS
110 120 130 140 150
MLYIQSLLDE WSFPATDPAV RAKYEARLAE IGVAALHAEL ARVDAAAAAS
160 170 180 190 200
ILPTDGRRIV RALEVVELTG EPFAASAPTI GAPRWDTAII GLDWDTAVLD
210 220 230 240 250
ERLAQRTDKM FADGLVREVV DLLERGLRDG VTAARALGYA QVLADLDAGG
260 270 280 290 300
DGSGAREPTF IGTRRYVRRQ RSWFRRDHRV VWLDGASEGL VDDALRVWRA

VS
Length:302
Mass (Da):32,577
Last modified:January 9, 2007 - v1
Checksum:iFA6921666A199A04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK71690.1
CP001663 Genomic DNA Translation: AFP39135.1
RefSeqiWP_003894118.1, NZ_CP009494.1
YP_887067.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK71690; ABK71690; MSMEG_2734
AFP39135; AFP39135; MSMEI_2667
GeneIDi4533335
KEGGimsb:LJ00_13595
msg:MSMEI_2667
msm:MSMEG_2734
PATRICifig|246196.19.peg.2702

Similar proteinsi

Entry informationi

Entry nameiMIAA_MYCS2
AccessioniPrimary (citable) accession number: A0QVX9
Secondary accession number(s): I7FC77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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