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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi32Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi68Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi102Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi176Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi180Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi183Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMSME246196:G1H7P-2728-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:MSMEG_2729, MSMEI_2662
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003743901 – 512tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST512

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_2729

Structurei

3D structure databases

ProteinModelPortaliA0QVX5
SMRiA0QVX5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 139MTTase N-terminalUniRule annotationAdd BLAST117
Domaini401 – 469TRAMUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW Bacteria
COG0621 LUCA
HOGENOMiHOG000224767
KOiK06168
OMAiKVCEHFH
OrthoDBiPOG091H00LG

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA_, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

A0QVX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTRDETAEA EGLRPAVGSS RVRTYQVRTY GCQMNVHDSE RLSGLLESAG
60 70 80 90 100
YQRAAEGTDA DIVVFNTCAV RENADNKLYG NLSHLAPRKQ ADPNMQIAVG
110 120 130 140 150
GCLAQKDRDA VLRRAPWVDV VFGTHNIGSL PTLLERARHN REAQVEIVEA
160 170 180 190 200
LQEFPSALPA SRESAYAAWV SISVGCNNTC TFCIVPSLRG KEVDRRPGDI
210 220 230 240 250
LAEVQSLVDQ GVLEITLLGQ NVNAYGVSFA DPELPRDRGA FAKLLRACGG
260 270 280 290 300
IDGLERVRFT SPHPAEFTDD VIEAMAATPN VCPTLHMPLQ SGSDRILKAM
310 320 330 340 350
RRSYRAERFL GIIDKVRAAI PHAAITTDLI VGFPGETEED FQATLDVVEQ
360 370 380 390 400
ARFAGAFTFQ YSKRPGTPAA DMPDQLPKSV VTERYQRLIE LQERISLEQN
410 420 430 440 450
REQVGRAVEL LVATGEGRKD ASTARMSGRA RDGRLVHFLP GDNEIRPGDI
460 470 480 490 500
VTTTVTGAAP HHLIADAPII ERRRTRAGDA HAAGQKPRTG VGLGMPRIGA
510
PAPSATAEGC GC
Length:512
Mass (Da):55,517
Last modified:January 9, 2007 - v1
Checksum:iFDC4DC90B0F830C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK73306.1
CP001663 Genomic DNA Translation: AFP39130.1
RefSeqiWP_011728547.1, NZ_CP009494.1
YP_887063.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK73306; ABK73306; MSMEG_2729
AFP39130; AFP39130; MSMEI_2662
GeneIDi4533482
KEGGimsb:LJ00_13570
msg:MSMEI_2662
msm:MSMEG_2729
PATRICifig|246196.19.peg.2696

Similar proteinsi

Entry informationi

Entry nameiMIAB_MYCS2
AccessioniPrimary (citable) accession number: A0QVX5
Secondary accession number(s): I7FC72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 9, 2007
Last modified: March 28, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health