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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi32Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi68Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi102Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi176Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi180Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi183Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:MSMEG_2729, MSMEI_2662
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003743901 – 512tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST512

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_2729.

Structurei

3D structure databases

ProteinModelPortaliA0QVX5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 139MTTase N-terminalUniRule annotationAdd BLAST117
Domaini401 – 469TRAMUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
KOiK06168.
OMAiCQMNFSD.
OrthoDBiPOG091H00LG.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QVX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTRDETAEA EGLRPAVGSS RVRTYQVRTY GCQMNVHDSE RLSGLLESAG
60 70 80 90 100
YQRAAEGTDA DIVVFNTCAV RENADNKLYG NLSHLAPRKQ ADPNMQIAVG
110 120 130 140 150
GCLAQKDRDA VLRRAPWVDV VFGTHNIGSL PTLLERARHN REAQVEIVEA
160 170 180 190 200
LQEFPSALPA SRESAYAAWV SISVGCNNTC TFCIVPSLRG KEVDRRPGDI
210 220 230 240 250
LAEVQSLVDQ GVLEITLLGQ NVNAYGVSFA DPELPRDRGA FAKLLRACGG
260 270 280 290 300
IDGLERVRFT SPHPAEFTDD VIEAMAATPN VCPTLHMPLQ SGSDRILKAM
310 320 330 340 350
RRSYRAERFL GIIDKVRAAI PHAAITTDLI VGFPGETEED FQATLDVVEQ
360 370 380 390 400
ARFAGAFTFQ YSKRPGTPAA DMPDQLPKSV VTERYQRLIE LQERISLEQN
410 420 430 440 450
REQVGRAVEL LVATGEGRKD ASTARMSGRA RDGRLVHFLP GDNEIRPGDI
460 470 480 490 500
VTTTVTGAAP HHLIADAPII ERRRTRAGDA HAAGQKPRTG VGLGMPRIGA
510
PAPSATAEGC GC
Length:512
Mass (Da):55,517
Last modified:January 9, 2007 - v1
Checksum:iFDC4DC90B0F830C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73306.1.
CP001663 Genomic DNA. Translation: AFP39130.1.
RefSeqiWP_011728547.1. NZ_CP009494.1.
YP_887063.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73306; ABK73306; MSMEG_2729.
AFP39130; AFP39130; MSMEI_2662.
GeneIDi4533482.
KEGGimsb:LJ00_13570.
msg:MSMEI_2662.
msm:MSMEG_2729.
PATRICi18077888. VBIMycSme59918_2696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73306.1.
CP001663 Genomic DNA. Translation: AFP39130.1.
RefSeqiWP_011728547.1. NZ_CP009494.1.
YP_887063.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QVX5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_2729.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73306; ABK73306; MSMEG_2729.
AFP39130; AFP39130; MSMEI_2662.
GeneIDi4533482.
KEGGimsb:LJ00_13570.
msg:MSMEI_2662.
msm:MSMEG_2729.
PATRICi18077888. VBIMycSme59918_2696.

Phylogenomic databases

eggNOGiENOG4105CIW. Bacteria.
COG0621. LUCA.
HOGENOMiHOG000224767.
KOiK06168.
OMAiCQMNFSD.
OrthoDBiPOG091H00LG.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIAB_MYCS2
AccessioniPrimary (citable) accession number: A0QVX5
Secondary accession number(s): I7FC72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.