A0QVR3 (DAPB_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate reductase
Short name=DHPR
EC=1.3.1.26

Gene names

Name:	dapB
Ordered Locus Names:	MSMEG_2664

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-2,3,4,5-tetrahydrodipicolinate + NAD(P)+ = (S)-2,3-dihydrodipicolinate + NAD(P)H. HAMAP MF_00102
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4. HAMAP MF_00102
Subcellular location	Cytoplasm By similarity HAMAP MF_00102.
Sequence similarities	Belongs to the dihydrodipicolinate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADPH binding Inferred from electronic annotation. Source: InterPro dihydrodipicolinate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Dihydrodipicolinate reductase HAMAP MF_00102		PRO_1000008597

Sequences

	Sequence		Length	Mass (Da)
	A0QVR3 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 4AAB4A4C9832612A	FASTA	245	25,710
	10 20 30 40 50 60 MRVGVLGARG KVGATMVAAV EAAEDLTFSA GVDAGDDLSL LTESKTEVVI DFTHPDVVMD 70 80 90 100 110 120 NLKFVIDNGI HAVVGTTGFT WERIEQVEAW VKAKPGASVL IAPNFAIGAV LSMHFAKQAA 130 140 150 160 170 180 KYFESVEIIE LHHPHKADAP SGTAARTAKL IAEARKGLPP NPDATSTGLD GARGADVDGI 190 200 210 220 230 240 PVHSVRLAGL VAHQEVLFGT QGETLTIRHD SLDRTSFVPG VLLAVRKISG LQGLTVGIEP LLDLS

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed: 18955433] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK71521.1.
RefSeq	YP_887001.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QVR3.
SMR	A0QVR3. Positions 1-244.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QVR3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000046616; EBMYCP00000044953; EBMYCG00000046611.
GeneID	4535928.
GenomeReviews	Gene locus MSMEG_2664 in contig CP000480_GR.
KEGG	msm:MSMEG_2664.
NMPDR	fig|246196.1.peg.2662.
PATRIC	18077756. VBIMycSme59918_2630.
TIGR	MSMEG_2664.
Phylogenomic databases
eggNOG	COG0289.
GeneTree	EBGT00050000017074.
HOGENOM	HBG594002.
OMA	DRASFMP.
ProtClustDB	PRK00048.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_2664-MONOMER.
Family and domain databases
HAMAP	MF_00102. DapB. [Tree]
InterPro	IPR022663. DapB_C. IPR000846. DapB_N. IPR022664. DapB_N_CS. IPR011770. Dihydrodipicolinate_Rdtase. IPR023940. Dihydrodipicolinate_Rdtase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00215.
PANTHER	PTHR20836. DapB_bac/pln. 1 hit.
Pfam	PF05173. DapB_C. 1 hit. PF01113. DapB_N. 1 hit. [Graphical view]
PIRSF	PIRSF000161. DHPR. 1 hit.
TIGRFAMs	TIGR00036. DapB. 1 hit.
PROSITE	PS01298. DAPB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPB_MYCS2

Accession

Primary (citable) accession number: A0QVR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program