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A0QVQ5

- PNP_MYCS2

UniProt

A0QVQ5 - PNP_MYCS2

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, gpsI, MSMEG_2656, MSMEI_2593
Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi526 – 5261Magnesium By similarity
Metal bindingi532 – 5321Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2656-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Synonyms:gpsI
Ordered Locus Names:MSMEG_2656, MSMEI_2593
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 763762Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329720Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_2656.

Structurei

3D structure databases

ProteinModelPortaliA0QVQ5.
SMRiA0QVQ5. Positions 13-726.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini592 – 65160KH
Add
BLAST
Domaini663 – 73270S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiTIAGKQD.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0QVQ5-1 [UniParc]FASTAAdd to Basket

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MSVVELEDGV YESTAVIDNG SFGTRTIRFE TGRLAQQAAG SAVAYLDDET    50
MLLSATTASK NPKDHFDFFP LTVDVEERMY AAGRIPGSFF RREGRPSTDA 100
ILTCRLIDRP LRPSFVDGLR NEIQVVVTVM SLDPKDLYDV LAINAASMST 150
QLAGLPFSGP VGGARIALID GTWVAFPTVE QLERAVFDMV VAGRIVGDGD 200
SADVAIMMVE AEATENVVEL VAGGAQAPTE AVVAEGLEAA KPFIKALCAA 250
QQELADRAAK PAGEYPVFPD YEADVYDAVA SVATEALAEA LTIAGKTERN 300
DRTDEIKVEV LERLAEPYAG REKEIGAAFR SLTKKLVRQR ILTDHFRIDG 350
RGITDIRALS AEVAVIPRAH GSALFERGET QILGVTTLDM IKMAQQIDSL 400
GPENTKRYMH HYNFPPYSTG ETGRVGSPKR REIGHGALAE RALVPVLPSI 450
EEFPYAIRQV SEALGSNGST SMGSVCASTL ALLNAGVPLK APVAGIAMGL 500
VSDDVDVDGK VEKRYVALTD ILGAEDAFGD MDFKVAGTKD FVTALQLDTK 550
LDGIPSQVLA GALSQAKDAR LTILDVMAEA IDRPDEMSPY APRITTIKVP 600
VDKIGEVIGP KGKMINSITE ETGAQISIED DGTVFVGAAD GLSAQAAIDK 650
INAIANPQLP KVGERFLGTV VKTTDFGAFV SLLPGRDGLV HISKLGKGKR 700
IAKVEDVVKV GDKLRVEIAD IDNRGKISLV LVAEESAESA ESAGDKGAEK 750
AEGAAADVTP AEA 763
Length:763
Mass (Da):81,027
Last modified:January 9, 2007 - v1
Checksum:i0F11A001A82BAC12
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK73464.1.
CP001663 Genomic DNA. Translation: AFP39061.1.
RefSeqiYP_006567356.1. NC_018289.1.
YP_886993.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73464; ABK73464; MSMEG_2656.
AFP39061; AFP39061; MSMEI_2593.
GeneIDi4532155.
KEGGimsg:MSMEI_2593.
msm:MSMEG_2656.
PATRICi18077740. VBIMycSme59918_2622.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000480 Genomic DNA. Translation: ABK73464.1 .
CP001663 Genomic DNA. Translation: AFP39061.1 .
RefSeqi YP_006567356.1. NC_018289.1.
YP_886993.1. NC_008596.1.

3D structure databases

ProteinModelPortali A0QVQ5.
SMRi A0QVQ5. Positions 13-726.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246196.MSMEG_2656.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK73464 ; ABK73464 ; MSMEG_2656 .
AFP39061 ; AFP39061 ; MSMEI_2593 .
GeneIDi 4532155.
KEGGi msg:MSMEI_2593.
msm:MSMEG_2656.
PATRICi 18077740. VBIMycSme59918_2622.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi TIAGKQD.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci MSME246196:GJ4Y-2656-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE.
    Strain: ATCC 700084 / mc(2)155.

Entry informationi

Entry nameiPNP_MYCS2
AccessioniPrimary (citable) accession number: A0QVQ5
Secondary accession number(s): I7G725
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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