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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi526MagnesiumUniRule annotation1
Metal bindingi532MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Synonyms:gpsI
Ordered Locus Names:MSMEG_2656, MSMEI_2593
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00003297202 – 763Polyribonucleotide nucleotidyltransferaseAdd BLAST762

Proteomic databases

PRIDEiA0QVQ5

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_2656

Structurei

3D structure databases

ProteinModelPortaliA0QVQ5
SMRiA0QVQ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini592 – 651KHUniRule annotationAdd BLAST60
Domaini663 – 732S1 motifUniRule annotationAdd BLAST70

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62 Bacteria
COG1185 LUCA
HOGENOMiHOG000218326
KOiK00962
OMAiRYMHNYN
OrthoDBiPOG091H00M0

Family and domain databases

Gene3Di3.30.230.70, 2 hits
HAMAPiMF_01595 PNPase, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR036345 ExoRNase_PH_dom2_sf
IPR014069 GPSI/PNP
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012340 NA-bd_OB-fold
IPR012162 PNPase
IPR027408 PNPase/RNase_PH_dom_sf
IPR015848 PNPase_PH_RNA-bd_bac/org-type
IPR036456 PNPase_PH_RNA-bd_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR022967 S1_dom
IPR003029 S1_domain
PANTHERiPTHR11252 PTHR11252, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 1 hit
PF03726 PNPase, 1 hit
PF01138 RNase_PH, 2 hits
PF00575 S1, 1 hit
PIRSFiPIRSF005499 PNPase, 1 hit
SMARTiView protein in SMART
SM00322 KH, 1 hit
SM00316 S1, 1 hit
SUPFAMiSSF46915 SSF46915, 1 hit
SSF50249 SSF50249, 1 hit
SSF54211 SSF54211, 2 hits
SSF54791 SSF54791, 1 hit
SSF55666 SSF55666, 2 hits
TIGRFAMsiTIGR03591 polynuc_phos, 1 hit
TIGR02696 pppGpp_PNP, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 1 hit
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0QVQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVVELEDGV YESTAVIDNG SFGTRTIRFE TGRLAQQAAG SAVAYLDDET
60 70 80 90 100
MLLSATTASK NPKDHFDFFP LTVDVEERMY AAGRIPGSFF RREGRPSTDA
110 120 130 140 150
ILTCRLIDRP LRPSFVDGLR NEIQVVVTVM SLDPKDLYDV LAINAASMST
160 170 180 190 200
QLAGLPFSGP VGGARIALID GTWVAFPTVE QLERAVFDMV VAGRIVGDGD
210 220 230 240 250
SADVAIMMVE AEATENVVEL VAGGAQAPTE AVVAEGLEAA KPFIKALCAA
260 270 280 290 300
QQELADRAAK PAGEYPVFPD YEADVYDAVA SVATEALAEA LTIAGKTERN
310 320 330 340 350
DRTDEIKVEV LERLAEPYAG REKEIGAAFR SLTKKLVRQR ILTDHFRIDG
360 370 380 390 400
RGITDIRALS AEVAVIPRAH GSALFERGET QILGVTTLDM IKMAQQIDSL
410 420 430 440 450
GPENTKRYMH HYNFPPYSTG ETGRVGSPKR REIGHGALAE RALVPVLPSI
460 470 480 490 500
EEFPYAIRQV SEALGSNGST SMGSVCASTL ALLNAGVPLK APVAGIAMGL
510 520 530 540 550
VSDDVDVDGK VEKRYVALTD ILGAEDAFGD MDFKVAGTKD FVTALQLDTK
560 570 580 590 600
LDGIPSQVLA GALSQAKDAR LTILDVMAEA IDRPDEMSPY APRITTIKVP
610 620 630 640 650
VDKIGEVIGP KGKMINSITE ETGAQISIED DGTVFVGAAD GLSAQAAIDK
660 670 680 690 700
INAIANPQLP KVGERFLGTV VKTTDFGAFV SLLPGRDGLV HISKLGKGKR
710 720 730 740 750
IAKVEDVVKV GDKLRVEIAD IDNRGKISLV LVAEESAESA ESAGDKGAEK
760
AEGAAADVTP AEA
Length:763
Mass (Da):81,027
Last modified:January 9, 2007 - v1
Checksum:i0F11A001A82BAC12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK73464.1
CP001663 Genomic DNA Translation: AFP39061.1
RefSeqiWP_011728507.1, NZ_CP009494.1
YP_886993.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK73464; ABK73464; MSMEG_2656
AFP39061; AFP39061; MSMEI_2593
GeneIDi4532155
KEGGimsb:LJ00_13220
msg:MSMEI_2593
msm:MSMEG_2656
PATRICifig|246196.19.peg.2622

Similar proteinsi

Entry informationi

Entry nameiPNP_MYCS2
AccessioniPrimary (citable) accession number: A0QVQ5
Secondary accession number(s): I7G725
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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