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Protein

Proline--tRNA ligase

Gene

proS

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS (By similarity).By similarity

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2621-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligase (EC:6.1.1.15)
Alternative name(s):
Prolyl-tRNA synthetase
Short name:
ProRS
Gene namesi
Name:proS
Ordered Locus Names:MSMEG_2621, MSMEI_2559
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Proline--tRNA ligasePRO_0000396807Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki173 – 173Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_2621.

Structurei

3D structure databases

ProteinModelPortaliA0QVM0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C90. Bacteria.
COG0442. LUCA.
HOGENOMiHOG000076893.
KOiK01881.
OMAiIQPAELW.
OrthoDBiPOG091H0290.

Family and domain databases

CDDicd00779. ProRS_core_prok. 1 hit.
Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR033730. ProRS_core_prok.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QVM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITRMSELFL RTLRDDPADA EVPSHKLLIR AGYVRAVGPG IYSWLPLGLR
60 70 80 90 100
VLRKIENVVR SEMNAIGAQE ILLPALLPRG PYETTNRWTE YGDTLFRLQD
110 120 130 140 150
RRNNDYLLGP THEELFTLTV KGEYSSYKDF PVILYQIQTK YRDEARPRAG
160 170 180 190 200
ILRGREFVMK DSYSFDVDDD GLKNAYYQHR EAYQRIFARL GVRYVIVSAV
210 220 230 240 250
SGAMGGSASE EFLAESEVGE DTFVRCVESG YAANVEAVIT RAPEAQPTEG
260 270 280 290 300
LPEAKVYDTP DTPTIATLVE WANSASLPQF EGRTVTAADT LKNVLLKTRE
310 320 330 340 350
PGGEWELLAV GVPGDREVDE KRLGAALEPA EFALLDDADF AANPFLVKGY
360 370 380 390 400
VGPKALQDNG VRYLVDPRVV HGSSWITGAD APNRHVVGLV AGRDFTPDGT
410 420 430 440 450
IEAAEVRDGD PSPDGAGVLT SARGIEIGHI FQLGRKYTDA FSADVLGEDG
460 470 480 490 500
KPLRLTMGSY GIGVSRLVAV IAEQQHDQLG LRWPSSVAPF DVHVVVANKD
510 520 530 540 550
AGARAGAAEL VADLDRLGHE VLFDDRQASP GVKFKDAELL GMPWIVVVGR
560 570 580
GWADGVVELR NRFTGETREI AADGAAAEIS SVLAG
Length:585
Mass (Da):63,844
Last modified:January 9, 2007 - v1
Checksum:i2907D834CCB9FFE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74453.1.
CP001663 Genomic DNA. Translation: AFP39027.1.
RefSeqiWP_003894002.1. NZ_CP009494.1.
YP_886958.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74453; ABK74453; MSMEG_2621.
AFP39027; AFP39027; MSMEI_2559.
GeneIDi4534796.
KEGGimsb:LJ00_13045.
msg:MSMEI_2559.
msm:MSMEG_2621.
PATRICi18077670. VBIMycSme59918_2587.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74453.1.
CP001663 Genomic DNA. Translation: AFP39027.1.
RefSeqiWP_003894002.1. NZ_CP009494.1.
YP_886958.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QVM0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_2621.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK74453; ABK74453; MSMEG_2621.
AFP39027; AFP39027; MSMEI_2559.
GeneIDi4534796.
KEGGimsb:LJ00_13045.
msg:MSMEI_2559.
msm:MSMEG_2621.
PATRICi18077670. VBIMycSme59918_2587.

Phylogenomic databases

eggNOGiENOG4105C90. Bacteria.
COG0442. LUCA.
HOGENOMiHOG000076893.
KOiK01881.
OMAiIQPAELW.
OrthoDBiPOG091H0290.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2621-MONOMER.

Family and domain databases

CDDicd00779. ProRS_core_prok. 1 hit.
Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR033730. ProRS_core_prok.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 2 hits.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYP_MYCS2
AccessioniPrimary (citable) accession number: A0QVM0
Secondary accession number(s): I7G0E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.