A0QVH7 (DXR_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Short name=DXP reductoisomerase
EC=1.1.1.267

Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase
2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	MSMEG_2578

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC NADPH binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_1000124102
Regions
	Nucleotide binding	18 – 47	30	NADP By similarity
Sites
	Metal binding	151	1	Divalent metal cation By similarity
	Metal binding	153	1	Divalent metal cation By similarity
	Metal binding	222	1	Divalent metal cation By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	200	1	Substrate By similarity
	Binding site	222	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QVH7 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 86DD2FED5018AEC2	FASTA	398	41,064
	10 20 30 40 50 60 MTTSAASGEP GRQRVLILGS TGSIGTQALE VIAANPDRFE VVGLAAGGGN PELLAAQRAQ 70 80 90 100 110 120 TGVAAVAVAD PAAAEAVGDV RYSGPDAVTR LVEDTEADVV LNALVGALGL QPTLAALATG 130 140 150 160 170 180 ARLALANKES LVAGGPLVLK AAAPGQIVPV DSEHSAMAQC LRGGTRAELD KIVLTASGGP 190 200 210 220 230 240 FLGWSAEDLK SVTPEQAGKH PTWSMGPMNT LNSATLVNKG LELIETHLLF GVDYDDIDVV 250 260 270 280 290 300 VHPQSIVHSM ATFTDGSTLA QASPPDMKLP IALALGWPDR IAGAAAACDF STASTWEFLP 310 320 330 340 350 360 LDNAVFPAVD LARFAGKQGG CLTAVYNSAN EEAAEAFLDG RIGFPDIVET VGDVLHAADR 370 380 390 WAAEPATVDD VLDAQRWARE QARGVVEQKS VRRGLVTK

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK73985.1.
RefSeq	YP_886915.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QVH7.
SMR	A0QVH7. Positions 11-386.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QVH7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000047137; EBMYCP00000045474; EBMYCG00000047132.
GeneID	4536334.
GenomeReviews	Gene locus MSMEG_2578 in contig CP000480_GR.
KEGG	msm:MSMEG_2578.
NMPDR	fig|246196.1.peg.2579.
PATRIC	18077584. VBIMycSme59918_2544.
TIGR	MSMEG_2578.
Phylogenomic databases
eggNOG	COG0743.
GeneTree	EBGT00050000016490.
HOGENOM	HBG430762.
OMA	IHSMVEY.
ProtClustDB	PRK05447.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_2578-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00099.
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_MYCS2

Accession

Primary (citable) accession number: A0QVH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program