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Protein

Uridylate kinase

Gene

pyrH

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP.UniRule annotation

Catalytic activityi

ATP + UMP = ADP + UDP.UniRule annotation

Enzyme regulationi

Inhibited by UTP.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64UMP; via amide nitrogenUniRule annotation1
Binding sitei65ATP; via amide nitrogenUniRule annotation1
Binding sitei69ATPUniRule annotation1
Binding sitei84UMPUniRule annotation1
Binding sitei178ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei181ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 26ATPUniRule annotation4
Nucleotide bindingi145 – 152UMPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00159; UER00275.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinaseUniRule annotation (EC:2.7.4.22UniRule annotation)
Short name:
UKUniRule annotation
Alternative name(s):
Uridine monophosphate kinaseUniRule annotation
Short name:
UMP kinaseUniRule annotation
Short name:
UMPKUniRule annotation
Gene namesi
Name:pyrHUniRule annotation
Ordered Locus Names:MSMEG_2540, MSMEI_2480
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00003238872 – 248Uridylate kinaseAdd BLAST247

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

STRINGi246196.MSMEG_2540.

Structurei

3D structure databases

ProteinModelPortaliA0QVD9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UMP kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C41. Bacteria.
COG0528. LUCA.
HOGENOMiHOG000047187.
KOiK09903.
OMAiRSRADYM.
OrthoDBiPOG091H02JZ.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_B. PyrH_B. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR015963. Uridylate_kinase_bac.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02075. pyrH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0QVD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSNVAGRA APIRPLYTRV LLKLGGEMFG GGQVGLDPDV VAQVARQIAE
60 70 80 90 100
VVRSGAQVAV VIGGGNFFRG AQLQQRGMER TRSDYMGMLG TVMNSLALQD
110 120 130 140 150
FLQKEGIDTR VQTAITMGQV AEPYIPLRAV RHLEKGRVVI FGAGMGLPYF
160 170 180 190 200
STDTTAAQRA LEIGAEVVLM AKAVDGVFTD DPRTNPDAEL ITAISHREVI
210 220 230 240
DRGLKVADAT AFSLCMDNGM PILVFNLLTS GNIARAVAGE KIGTLVTT
Length:248
Mass (Da):26,363
Last modified:January 9, 2007 - v1
Checksum:i883175F5E0DC2E03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70978.1.
CP001663 Genomic DNA. Translation: AFP38948.1.
RefSeqiWP_003893896.1. NZ_CP009494.1.
YP_886877.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70978; ABK70978; MSMEG_2540.
AFP38948; AFP38948; MSMEI_2480.
GeneIDi4535313.
KEGGimsb:LJ00_12645.
msg:MSMEI_2480.
msm:MSMEG_2540.
PATRICi18077508. VBIMycSme59918_2506.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK70978.1.
CP001663 Genomic DNA. Translation: AFP38948.1.
RefSeqiWP_003893896.1. NZ_CP009494.1.
YP_886877.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QVD9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_2540.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK70978; ABK70978; MSMEG_2540.
AFP38948; AFP38948; MSMEI_2480.
GeneIDi4535313.
KEGGimsb:LJ00_12645.
msg:MSMEI_2480.
msm:MSMEG_2540.
PATRICi18077508. VBIMycSme59918_2506.

Phylogenomic databases

eggNOGiENOG4105C41. Bacteria.
COG0528. LUCA.
HOGENOMiHOG000047187.
KOiK09903.
OMAiRSRADYM.
OrthoDBiPOG091H02JZ.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00275.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_B. PyrH_B. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR015963. Uridylate_kinase_bac.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02075. pyrH_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPYRH_MYCS2
AccessioniPrimary (citable) accession number: A0QVD9
Secondary accession number(s): I7FJN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.