A0QVB9 (EFTS_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Ts
Short name=EF-Ts

Gene names

Name:	tsf
Ordered Locus Names:	MSMEG_2520

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. HAMAP MF_00050
Subcellular location	Cytoplasm HAMAP MF_00050.
Sequence similarities	Belongs to the EF-Ts family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Elongation factor
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 275	274	Elongation factor Ts HAMAP MF_00050		PRO_1000006128
Regions
	Region	76 – 79	4	Involved in Mg(2+) ion dislocation from EF-Tu By similarity
Amino acid modifications
	Cross-link	36		Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup) HAMAP MF_00050

Sequences

	Sequence		Length	Mass (Da)
	A0QVB9 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: BB77B8F24C6AA8A7	FASTA	275	29,405
	10 20 30 40 50 60 MANYTAADVK RLRELTGAGM LDSKNALVEA DGDFDKAVEL LRIKGAKDVG KRAERATAEG 70 80 90 100 110 120 LVAAKDGALI ELNSETDFVA KNAEFQALAD QIVAAAVAAK ANDIETLKAA KTGDTTVEQA 130 140 150 160 170 180 IADLSAKIGE KLELRRAAYF DGTVEAYLHK RAADLPPAVG VLVEYQAGDA DKGKEAAHAV 190 200 210 220 230 240 ALQIAALKAK YLTREDVPED IVANERRIAE ETARNEGKPE QALPKIVEGR VTGFYKDVVL 250 260 270 LDQPSVSDNK KTVKALLDEA GVTVTRFVRF EVGQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed: 18955433] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE.
[3]	"Expansion of the mycobacterial 'PUPylome'." Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V., Barry C.E. III, Bark S., Dorrestein P.C. Mol. Biosyst. 6:376-385(2010) [PubMed: 20094657] [Abstract] Cited for: PUPYLATION AT LYS-36, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK70376.1.
RefSeq	YP_886857.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QVB9.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QVB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000045011; EBMYCP00000043348; EBMYCG00000045006.
GeneID	4534407.
GenomeReviews	Gene locus MSMEG_2520 in contig CP000480_GR.
KEGG	msm:MSMEG_2520.
NMPDR	fig|246196.1.peg.6905.
PATRIC	18077470. VBIMycSme59918_2487.
TIGR	MSMEG_2520.
Phylogenomic databases
eggNOG	COG0264.
GeneTree	EBGT00050000016347.
HOGENOM	HBG713289.
OMA	YLHGTRI.
PhylomeDB	A0QVB9.
ProtClustDB	PRK09377.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_2520-MONOMER.
Family and domain databases
HAMAP	MF_00050. EF_Ts. [Tree]
InterPro	IPR001816. Transl_elong_EFTs/EF1B. IPR014039. Transl_elong_EFTs/EF1B_dimer. IPR018101. Transl_elong_Ts_CS. IPR009060. UBA-like. IPR000449. UBA/transl_elong_EF1B_N. [Graphical view]
Gene3D	G3DSA:3.30.479.20. Transl_elong_EFTs/EF1B_dimer. 3 hits.
KO	K02357.
PANTHER	PTHR11741. Transl_elong_EFTs/EF1B. 1 hit.
Pfam	PF00889. EF_TS. 1 hit. PF00627. UBA. 1 hit. [Graphical view]
SUPFAM	SSF54713. EF_TS. 1 hit. SSF46934. UBA_like. 1 hit.
TIGRFAMs	TIGR00116. Tsf. 1 hit.
PROSITE	PS01126. EF_TS_1. 1 hit. PS01127. EF_TS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTS_MYCS2

Accession

Primary (citable) accession number: A0QVB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program