A0QV44 (RNH2_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	MSMEG_2442

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Ribonuclease HII HAMAP MF_00052_B		PRO_1000031164
Sites
	Metal binding	36	1	Divalent metal cation By similarity
	Metal binding	37	1	Divalent metal cation By similarity
	Metal binding	130	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QV44 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 5975EDBBBA392761	FASTA	272	29,300
	10 20 30 40 50 60 MPGSWPPRTV IRKSSGLRTL ESALYRNGLG PVAGVDEVGR GACAGPLVVA ACVLGPNRLE 70 80 90 100 110 120 SLAALDDSKK LGEKERERLF PLIRRYALAY HVVFIPSHEV DRRGVHVANI EGMRRAVAGL 130 140 150 160 170 180 SVRPGYVLSD GFRVPGLSVP SLPVIGGDAA AACIAAASVL AKVSRDRLMV EMEEQHPGYG 190 200 210 220 230 240 FAEHKGYITP AHTAALTRHG PCVEHRYSYV NVRRAAEATG VRWASDRVGV RWSTESAAET 250 260 270 EEVRRALEAF EVEWVTAPVE HEDAACAKMG SG

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK73838.1.
RefSeq	YP_886782.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QV44.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QV44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000041141; EBMYCP00000039478; EBMYCG00000041136.
GeneID	4536492.
GenomeReviews	Gene locus MSMEG_2442 in contig CP000480_GR.
KEGG	msm:MSMEG_2442.
PATRIC	18077310. VBIMycSme59918_2407.
TIGR	MSMEG_2442.
Phylogenomic databases
eggNOG	COG0164.
GeneTree	EBGT00050000016477.
HOGENOM	HBG584843.
OMA	RLGPTPI.
PhylomeDB	A0QV44.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_2442-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_MYCS2

Accession

Primary (citable) accession number: A0QV44

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program