A0QV40 (A0QV40_MYCS2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
EC=2.1.1.228 HAMAP MF_00605

Alternative name(s):
M1G-methyltransferase HAMAP MF_00605
tRNA [GM37] methyltransferase HAMAP MF_00605

Gene names

Name:	trmD HAMAP MF_00605
Ordered Locus Names:	MSMEG_2438

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) EMBL ABK73206.1

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm By similarity HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. HAMAP MF_00605

Ontologies

Keywords
Biological process	tRNA processing HAMAP MF_00605
Cellular component	Cytoplasm HAMAP MF_00605
Ligand	S-adenosyl-L-methionine HAMAP MF_00605
Molecular function	Methyltransferase HAMAP MF_00605 EMBL ABK73206.1 Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity HAMAP MF_00605
Sites
	Binding site	109	1	S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP MF_00605

Sequences

	Sequence		Length	Mass (Da)
	A0QV40 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 99C5EB45A4723CCE	FASTA	225	24,969
	10 20 30 40 50 60 MRIDVISIFP AYLDPIRQSL PGKAIDAGIV SVEVHDLRNW THDVHRSVDD SPYGGGPGMV 70 80 90 100 110 120 MKAPVWGEAL DEICSEETLL VVPTPAGRLF DQRTAQRWST ERHLVFACGR YEGIDQRVVD 130 140 150 160 170 180 DAARRMRVEE VSIGDYVLNG GEAATLVMVE AVVRLLPDVL GNPASHQQDS HSDGLLEGPS 190 200 210 220 YTRPPSWRGL DVPPVLLSGD HAKVAAWRHE QALQRTRERR PDLLD

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK73206.1.
RefSeq	YP_886778.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QV40.
ModBase	Search...
Protein-protein interaction databases
STRING	A0QV40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000042340; EBMYCP00000040677; EBMYCG00000042335.
GeneID	4534479.
GenomeReviews	Gene locus MSMEG_2438 in contig CP000480_GR.
KEGG	msm:MSMEG_2438.
NMPDR	fig|246196.1.peg.2436.
PATRIC	18077302. VBIMycSme59918_2403.
TIGR	MSMEG_2438.
Phylogenomic databases
eggNOG	COG0336.
GeneTree	EBGT00050000016420.
HOGENOM	HBG285805.
OMA	VCGRFEG.
PhylomeDB	A0QV40.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0QV40_MYCS2

Accession

Primary (citable) accession number: A0QV40

Entry history

Integrated into UniProtKB/TrEMBL:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)