D-alanine--D-alanine ligase - Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Contribute

Send feedback

Read comments (?) or add your own

A0QUZ7 (DDL_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 53. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4

Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase

Gene names

Name:	ddl
Ordered Locus Names:	MSMEG_2395, MSMEI_2335

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium ›

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 373

373

D-alanine--D-alanine ligase HAMAP-Rule MF_00047

PRO_1000030469

Regions

Domain

156 – 363

208

ATP-grasp

Nucleotide binding

184 – 239

ATP By similarity

Sites

Metal binding

318

Magnesium or manganese 1 By similarity

Metal binding

330

Magnesium or manganese 1 By similarity

Metal binding

330

Magnesium or manganese 2 By similarity

Metal binding

332

Magnesium or manganese 2 By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A0QUZ7 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: FE7151D858C39D43
Show »

FASTA

373

39,232

        10         20         30         40         50         60 
MTAPNHPPGR TRVAVVYGGR SSEHAISCVS AGSILRNLDP ERFEVVAIGI TPDGSWVLTD 

        70         80         90        100        110        120 
GRPETLAITD GKLPAVTEAS GTELALPAAP NRSGQLLALG NGPGEILAAV DVVFPVLHGP 

       130        140        150        160        170        180 
YGEDGTIQGL LELAGVPYVG SGVLASAAGM DKEYTKKLLA AEGLPIGDQV VLRPGVETLD 

       190        200        210        220        230        240 
LEQRERLGLP VFVKPARGGS SIGVSRVTAW DELPAAVALA RRHDPKVIVE AAVIGRELEC 

       250        260        270        280        290        300 
GVLEFPDGRL EASTVGEIRV AGVRGREDGF YDFATKYLED AAELDVPAKV DDDVADEIRQ 

       310        320        330        340        350        360 
LAVRAFTAIG CQGLARVDFF LTDDGPVINE INTMPGFTTI SMYPRMWAAG GIDYPTLLAA 

       370 
MVDTAIARGT GLR

« Hide

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK70518.1. CP001663 Genomic DNA. Translation: AFP38803.1.
RefSeq	YP_006567098.1. NC_018289.1. YP_886735.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QUZ7.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_2395.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK70518; ABK70518; MSMEG_2395.
GeneID	13428852. 4532086.
KEGG	msg:MSMEI_2335. msm:MSMEG_2395.
PATRIC	18077214. VBIMycSme59918_2359.
Phylogenomic databases
eggNOG	COG1181.
HOGENOM	HOG000011593.
KO	K01921.
OMA	LLHGPFG.
ProtClustDB	PRK01966.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-2395-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_MYCS2

Accession

Primary (citable) accession number: A0QUZ7
Secondary accession number(s): I7FJ47

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents