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Protein

2-phospho-L-lactate guanylyltransferase

Gene

cofC

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Guanylyltransferase that probably catalyzes the activation of 2-phospho-L-lactate (LP) as (2S)-lactyl-2-diphospho-5'-guanosine (LPPG), via the condensation of LP with GTP. Is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.1 Publication

Catalytic activityi

(2S)-2-phospholactate + GTP = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate.UniRule annotation

Pathwayi: coenzyme F420 biosynthesis

This protein is involved in the pathway coenzyme F420 biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway coenzyme F420 biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
2-phospho-L-lactate guanylyltransferaseUniRule annotation (EC:2.7.7.68UniRule annotation)
Short name:
LP guanylyltransferaseUniRule annotation
Gene namesi
Name:cofCUniRule annotation
Ordered Locus Names:MSMEG_2392, MSMEI_2332
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene accumulate 7,8-didemethyl-8-hydroxy-5-deazariboflavin (Fo) to five times the wild-type levels but do not produce F420. They are also unable to decolorize triphenylmethane dye, and are sensitive to oxidative stress caused by the redox-cycling agents plumbagin and menadione.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003986951 – 2212-phospho-L-lactate guanylyltransferaseAdd BLAST221

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_2392.

Structurei

3D structure databases

ProteinModelPortaliA0QUZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CofC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105ME0. Bacteria.
COG1920. LUCA.
HOGENOMiHOG000223639.
KOiK14941.
OMAiAMLVDTI.
OrthoDBiPOG091H0B3J.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_02114. CofC. 1 hit.
InterProiIPR002835. Coenzyme_F420_CofC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01983. CofC. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03552. F420_cofC. 1 hit.

Sequencei

Sequence statusi: Complete.

A0QUZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGRRTPGAA SDGSAGGQAA VIIAVKRLTA AKTRLAPIFS AATREEVVLA
60 70 80 90 100
MLVDTITAAS TVAPVTVVTP DEVAADAARQ LGAGVLADPT PEGHHNPLNN
110 120 130 140 150
AIMAAEEHLR AGTPNIVVLQ GDLPAMQPRE LAEALAAART YPRSFVGDRH
160 170 180 190 200
GTGTSALFAF GVPLQPRFGA DSATHHRHSG AIELTGAWPG LRCDIDTAED
210 220
LRTARRLGVG PATAQAIAAH R
Length:221
Mass (Da):22,750
Last modified:January 9, 2007 - v1
Checksum:i7E231809F648F8B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73289.1.
CP001663 Genomic DNA. Translation: AFP38800.1.
RefSeqiWP_003893758.1. NZ_CP009494.1.
YP_886732.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK73289; ABK73289; MSMEG_2392.
AFP38800; AFP38800; MSMEI_2332.
GeneIDi4535871.
KEGGimsb:LJ00_11895.
msg:MSMEI_2332.
msm:MSMEG_2392.
PATRICi18077208. VBIMycSme59918_2356.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK73289.1.
CP001663 Genomic DNA. Translation: AFP38800.1.
RefSeqiWP_003893758.1. NZ_CP009494.1.
YP_886732.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QUZ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_2392.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK73289; ABK73289; MSMEG_2392.
AFP38800; AFP38800; MSMEI_2332.
GeneIDi4535871.
KEGGimsb:LJ00_11895.
msg:MSMEI_2332.
msm:MSMEG_2392.
PATRICi18077208. VBIMycSme59918_2356.

Phylogenomic databases

eggNOGiENOG4105ME0. Bacteria.
COG1920. LUCA.
HOGENOMiHOG000223639.
KOiK14941.
OMAiAMLVDTI.
OrthoDBiPOG091H0B3J.

Enzyme and pathway databases

UniPathwayiUPA00071.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_02114. CofC. 1 hit.
InterProiIPR002835. Coenzyme_F420_CofC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01983. CofC. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03552. F420_cofC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOFC_MYCS2
AccessioniPrimary (citable) accession number: A0QUZ4
Secondary accession number(s): I7FB84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.