A0QUY7 (SYE_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	MSMEG_2383, MSMEI_2323

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_0000330980
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	256 – 260	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	259	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QUY7 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 4A7B779612605BBC	FASTA	486	54,019
	10 20 30 40 50 60 MTTKKVRVRF CPSPTGTPHV GLVRTALFNW AYARHTGGDF VFRIEDTDAA RDSDESYAAI 70 80 90 100 110 120 LDALRWLGMD WDEGPEVGGP YEPYRQSQRR EIYRDVVARL LEAGEVYEAY STPEEVEARH 130 140 150 160 170 180 LAAGRNPKLG YDNYDRDLTD AQRKAFADEG RRPVLRLRMP DEDLSWNDLV RGPTTFAAGS 190 200 210 220 230 240 VPDFAITRSN GDPLYTLVNP VDDALMKITH VLRGEDILPS TPRQIALYRA LMRIGVAEFV 250 260 270 280 290 300 PEFAHLPSVL GEGNKKLSKR DPQSNLFLHR DRGFIPEGLL NYLALLGWGI ADDHDVFSLD 310 320 330 340 350 360 EMVAAFDVAD VNSNPARFDQ KKADAINAEH IRMLAPEDFT ARLREYFVTH GYDTTLDDAA 370 380 390 400 410 420 FAEAAALVQT RVVVLGDAWG LLKFFNDDAY EIDEKSAAKE LKPESAAVLD AALSALEAVG 430 440 450 460 470 480 DWTTPAIEAA LKTALLEGLE LKPRKAFGPI RVAVTGAAVS PPLFESMELL GRDRSLARLR AARDRV

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK74186.1. CP001663 Genomic DNA. Translation: AFP38793.1.
RefSeq	YP_006567088.1. NC_018289.1. YP_886725.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QUY7.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_2383.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK74186; ABK74186; MSMEG_2383.
GeneID	13428840. 4533604.
KEGG	msg:MSMEI_2323. msm:MSMEG_2383.
PATRIC	18077190. VBIMycSme59918_2349.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252722.
KO	K01885.
OMA	RYRDFKG.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-2383-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_MYCS2

Accession

Primary (citable) accession number: A0QUY7
Secondary accession number(s): I7FJ34

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program