Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acetolactate synthase small subunit

Gene

ilvH

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Pathway: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB), Acetolactate synthase small subunit (ilvH)
  2. Ketol-acid reductoisomerase (ilvC), Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD), Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (LJ00_21200), Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathway: L-valine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvB), Acetolactate synthase small subunit (ilvH)
  2. Ketol-acid reductoisomerase (ilvC), Ketol-acid reductoisomerase (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD), Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (LJ00_21200), Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2373-MONOMER.
UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase small subunit (EC:2.2.1.6)
Alternative name(s):
Acetohydroxy-acid synthase small subunit
Short name:
AHAS
Short name:
ALS
Gene namesi
Name:ilvH
Synonyms:ilvN
Ordered Locus Names:MSMEG_2373, MSMEI_2313
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
ProteomesiUP000006158 Componenti: Chromosome UP000000757 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 170170Acetolactate synthase small subunitPRO_0000396815Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki46 – 46Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Dimer of large and small chains.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_2373.

Structurei

3D structure databases

ProteinModelPortaliA0QUX7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 8375ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0440.
HOGENOMiHOG000046747.
KOiK01653.
OMAiLFRAHVV.
OrthoDBiEOG6X3W8W.

Family and domain databases

InterProiIPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT_dom.
[Graphical view]
PfamiPF10369. ALS_ss_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00119. acolac_sm. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QUX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNGTPTHTL SVLVEDKPGV LARVSSLFSR RGFNIQSLAV GATEQKDMSR
60 70 80 90 100
MTIVVSVEDS PLEQITKQLN KLINVIKIVE QEEDNSVSRE LALIKVRADA
110 120 130 140 150
TTRGQIIEAV NLFRAKVVDV STESLTIEAT GTPEKLEALL RVLEPYGIRE
160 170
IAQSGVVSVS RGPRGIGAAK
Length:170
Mass (Da):18,395
Last modified:January 9, 2007 - v1
Checksum:iDDA5829FE67BC49D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72789.1.
CP001663 Genomic DNA. Translation: AFP38783.1.
RefSeqiWP_003893741.1. NC_018289.1.
YP_006567078.1. NC_018289.1.
YP_886715.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK72789; ABK72789; MSMEG_2373.
AFP38783; AFP38783; MSMEI_2313.
GeneIDi4532109.
KEGGimsm:MSMEG_2373.
PATRICi18077170. VBIMycSme59918_2339.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK72789.1.
CP001663 Genomic DNA. Translation: AFP38783.1.
RefSeqiWP_003893741.1. NC_018289.1.
YP_006567078.1. NC_018289.1.
YP_886715.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QUX7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_2373.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK72789; ABK72789; MSMEG_2373.
AFP38783; AFP38783; MSMEI_2313.
GeneIDi4532109.
KEGGimsm:MSMEG_2373.
PATRICi18077170. VBIMycSme59918_2339.

Phylogenomic databases

eggNOGiCOG0440.
HOGENOMiHOG000046747.
KOiK01653.
OMAiLFRAHVV.
OrthoDBiEOG6X3W8W.

Enzyme and pathway databases

UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.
BioCyciMSME246196:GJ4Y-2373-MONOMER.

Family and domain databases

InterProiIPR004789. Acetalactate_synth_ssu.
IPR019455. Acetolactate_synth_ssu_C.
IPR002912. ACT_dom.
[Graphical view]
PfamiPF10369. ALS_ss_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00119. acolac_sm. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. Cited for: PUPYLATION AT LYS-46, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiILVH_MYCS2
AccessioniPrimary (citable) accession number: A0QUX7
Secondary accession number(s): I7FJ23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: January 9, 2007
Last modified: June 24, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.