A0QUX7 (ILVH_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetolactate synthase small subunit
EC=2.2.1.6

Alternative name(s):
Acetohydroxy-acid synthase small subunit
Short name=AHAS
Short name=ALS

Gene names

Name:	ilvH
Synonyms:	ilvN
Ordered Locus Names:	MSMEG_2373, MSMEI_2313

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of large and small chains By similarity.
Sequence similarities	Belongs to the acetolactate synthase small subunit family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Molecular function	Transferase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway valine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	acetolactate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Acetolactate synthase small subunit		PRO_0000396815
Regions
	Domain	8 – 73	66	ACT
Amino acid modifications
	Cross-link	46		Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sequences

	Sequence		Length	Mass (Da)
	A0QUX7 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: DDA5829FE67BC49D	FASTA	170	18,395
	10 20 30 40 50 60 MSNGTPTHTL SVLVEDKPGV LARVSSLFSR RGFNIQSLAV GATEQKDMSR MTIVVSVEDS 70 80 90 100 110 120 PLEQITKQLN KLINVIKIVE QEEDNSVSRE LALIKVRADA TTRGQIIEAV NLFRAKVVDV 130 140 150 160 170 STESLTIEAT GTPEKLEALL RVLEPYGIRE IAQSGVVSVS RGPRGIGAAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Expansion of the mycobacterial 'PUPylome'." Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V., Barry C.E. III, Bark S., Dorrestein P.C. Mol. Biosyst. 6:376-385(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PUPYLATION AT LYS-46, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK72789.1. CP001663 Genomic DNA. Translation: AFP38783.1.
RefSeq	YP_006567078.1. NC_018289.1. YP_886715.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QUX7.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_2373.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK72789; ABK72789; MSMEG_2373.
GeneID	13428830. 4532109.
KEGG	msg:MSMEI_2313. msm:MSMEG_2373.
PATRIC	18077170. VBIMycSme59918_2339.
Phylogenomic databases
eggNOG	COG0440.
HOGENOM	HOG000046747.
KO	K01653.
OMA	RMTIVVN.
ProtClustDB	PRK11895.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-2373-MONOMER.
UniPathway	UPA00047; UER00055. UPA00049; UER00059.
Family and domain databases
InterPro	IPR004789. Acetalactate_synth_ssu. IPR019455. Acetolactate_synth_ssu_C. [Graphical view]
Pfam	PF10369. ALS_ss_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00119. acolac_sm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVH_MYCS2

Accession

Primary (citable) accession number: A0QUX7
Secondary accession number(s): I7FJ23

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program