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Protein

DNA ligase B

Gene

ligB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation1 Publication

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei212ATPUniRule annotation1
Active sitei214N6-AMP-lysine intermediateUniRule annotation1
Binding sitei219ATPUniRule annotation1
Binding sitei234ATPUniRule annotation1
Binding sitei263ATPUniRule annotation1
Binding sitei299ATPUniRule annotation1
Binding sitei371ATPUniRule annotation1
Binding sitei377ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase B (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligB
Ordered Locus Names:MSMEG_2277, MSMEI_2220
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Not essential for growth, no effect on NHEJ. In quadruple ligB-ligC1-ligC2-ligD deletions NHEJ on blunt and 5'-overhangs is 0.22 and 0.12% of wild-type respectively; only 4-fold decrease in 3'-overhang NHEJ.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003652271 – 509DNA ligase BAdd BLAST509

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_2277

Structurei

3D structure databases

ProteinModelPortaliA0QUP1
SMRiA0QUP1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RYT Bacteria
COG1793 LUCA
HOGENOMiHOG000036008
KOiK10747
OMAiWLFEESY
OrthoDBiPOG091H0BGA

Family and domain databases

Gene3Di1.10.3260.10, 1 hit
HAMAPiMF_00407 DNA_ligase, 1 hit
InterProiView protein in InterPro
IPR022865 DNA_ligae_ATP-dep_bac/arc
IPR000977 DNA_ligase_ATP-dep
IPR012309 DNA_ligase_ATP-dep_C
IPR012310 DNA_ligase_ATP-dep_cent
IPR016059 DNA_ligase_ATP-dep_CS
IPR012308 DNA_ligase_ATP-dep_N
IPR036599 DNA_ligase_N_sf
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF04679 DNA_ligase_A_C, 1 hit
PF01068 DNA_ligase_A_M, 1 hit
PF04675 DNA_ligase_A_N, 1 hit
SUPFAMiSSF117018 SSF117018, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00574 dnl1, 1 hit
PROSITEiView protein in PROSITE
PS00697 DNA_LIGASE_A1, 1 hit
PS00333 DNA_LIGASE_A2, 1 hit
PS50160 DNA_LIGASE_A3, 1 hit

Sequencei

Sequence statusi: Complete.

A0QUP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLADVAAAST EVAASSARLV KIERIATLLA RSAAEDDTQA VAVIVSWLSG
60 70 80 90 100
ELPQRQIGVG WAALRTLPPP AATPSLTVDD VDDRLSTIKA VAGKGSQATR
110 120 130 140 150
AGLVHELFSA ATDPEQKFLR HLLSGELRQG ALAGVMADAV AKAAGLPAAE
160 170 180 190 200
IRRAAMLAGN LPAVAAAAVT GGRAALADFR LRVGRPVGPM LAQTATSVDD
210 220 230 240 250
ALQRLGGTAV LEAKLDGARV QIHRSGCDVS IYTRSLDDVT HRLPEVVEAT
260 270 280 290 300
LALPATELIA DAEAIALRPD GRPHLFQVTA ARFGRKDPGD LGPLSVFFFD
310 320 330 340 350
LLHVDGRDLL DLPTEERFGA LDALVRQDQR VDRLVTTDTV AAQEFLERTL
360 370 380 390 400
AAGHEGVMAK SPHAAYEAGR RGAGWLKVKP VHTLDLVVLA VEWGSGRRQG
410 420 430 440 450
KLSNIHLGAR DPDSSGFVML GKTFKGMTDA MLEWQTQRFL ELADGPTDGY
460 470 480 490 500
VVHLRPEQVV EIAFDGVQRS SRYPAGMALR FVRVLRYRDD KSPAEADTVE

TVRRFYERD
Length:509
Mass (Da):54,594
Last modified:January 9, 2007 - v1
Checksum:iE5C3AADF5B8F311C
GO

Sequence cautioni

The sequence AFP38690 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK74122.1
CP001663 Genomic DNA Translation: AFP38690.1 Different initiation.
RefSeqiWP_011728274.1, NZ_CP009494.1
YP_886629.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK74122; ABK74122; MSMEG_2277
AFP38690; AFP38690; MSMEI_2220
GeneIDi4536201
KEGGimsg:MSMEI_2220
msm:MSMEG_2277
PATRICifig|246196.19.peg.2242

Similar proteinsi

Entry informationi

Entry nameiDNLI_MYCS2
AccessioniPrimary (citable) accession number: A0QUP1
Secondary accession number(s): I7FAW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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