Probable DNA ligase - Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

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A0QUP1 (DNLI_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 60. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	MSMEG_2277, MSMEI_2220

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium ›

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.
Sequence caution	The sequence AFP38690.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 509

509

Probable DNA ligase HAMAP-Rule MF_00407

PRO_0000365227

Sites

Active site

214

N6-AMP-lysine intermediate By similarity

Binding site

212

ATP By similarity

Binding site

219

ATP By similarity

Binding site

234

ATP By similarity

Binding site

263

ATP By similarity

Binding site

299

ATP By similarity

Binding site

371

ATP By similarity

Binding site

377

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A0QUP1 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: E5C3AADF5B8F311C
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FASTA

509

54,594

        10         20         30         40         50         60 
MLADVAAAST EVAASSARLV KIERIATLLA RSAAEDDTQA VAVIVSWLSG ELPQRQIGVG 

        70         80         90        100        110        120 
WAALRTLPPP AATPSLTVDD VDDRLSTIKA VAGKGSQATR AGLVHELFSA ATDPEQKFLR 

       130        140        150        160        170        180 
HLLSGELRQG ALAGVMADAV AKAAGLPAAE IRRAAMLAGN LPAVAAAAVT GGRAALADFR 

       190        200        210        220        230        240 
LRVGRPVGPM LAQTATSVDD ALQRLGGTAV LEAKLDGARV QIHRSGCDVS IYTRSLDDVT 

       250        260        270        280        290        300 
HRLPEVVEAT LALPATELIA DAEAIALRPD GRPHLFQVTA ARFGRKDPGD LGPLSVFFFD 

       310        320        330        340        350        360 
LLHVDGRDLL DLPTEERFGA LDALVRQDQR VDRLVTTDTV AAQEFLERTL AAGHEGVMAK 

       370        380        390        400        410        420 
SPHAAYEAGR RGAGWLKVKP VHTLDLVVLA VEWGSGRRQG KLSNIHLGAR DPDSSGFVML 

       430        440        450        460        470        480 
GKTFKGMTDA MLEWQTQRFL ELADGPTDGY VVHLRPEQVV EIAFDGVQRS SRYPAGMALR 

       490        500 
FVRVLRYRDD KSPAEADTVE TVRRFYERD

« Hide

References

[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK74122.1. CP001663 Genomic DNA. Translation: AFP38690.1. Different initiation.
RefSeq	YP_006566985.1. NC_018289.1. YP_886629.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QUP1.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_2277.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK74122; ABK74122; MSMEG_2277.
GeneID	13428737. 4536201.
KEGG	msg:MSMEI_2220. msm:MSMEG_2277.
PATRIC	18076976. VBIMycSme59918_2242.
Phylogenomic databases
eggNOG	COG1793.
HOGENOM	HOG000036008.
KO	K01971.
OMA	ARVQVHK.
ProtClustDB	PRK03180.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-2277-MONOMER.
Family and domain databases
Gene3D	1.10.3260.10. 1 hit. 2.40.50.140. 1 hit.
HAMAP	MF_00407. DNA_ligase.
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_MYCS2

Accession

Primary (citable) accession number: A0QUP1
Secondary accession number(s): I7FAW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents