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Protein

Secreted chorismate mutase

Gene

MSMEG_2111

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the Claisen rearrangement of chorismate to prephenate. May play some role in the pathogenicity.1 Publication

Catalytic activityi

Chorismate = prephenate.

Pathwayi: prephenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes prephenate from chorismate.
Proteins known to be involved in this subpathway in this organism are:
  1. Intracellular chorismate mutase (MSMEG_5536), Secreted chorismate mutase (MSMEG_2111)
This subpathway is part of the pathway prephenate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes prephenate from chorismate, the pathway prephenate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361SubstrateBy similarity
Binding sitei47 – 471SubstrateBy similarity
Binding sitei56 – 561SubstrateBy similarity
Binding sitei121 – 1211SubstrateBy similarity

GO - Molecular functioni

  • chorismate mutase activity Source: UniProtKB

GO - Biological processi

  • chorismate metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2111-MONOMER.
BRENDAi5.4.99.5. 3512.
UniPathwayiUPA00120; UER00203.

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted chorismate mutase (EC:5.4.99.5)
Short name:
CM
Gene namesi
Ordered Locus Names:MSMEG_2111, MSMEI_2064
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 181161Secreted chorismate mutasePRO_0000414905Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi147 ↔ 180By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_2111.

Structurei

3D structure databases

ProteinModelPortaliA0QU81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 10080Chorismate mutasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni59 – 635Substrate bindingBy similarity
Regioni92 – 965Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 chorismate mutase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105WWF. Bacteria.
COG1605. LUCA.
HOGENOMiHOG000257623.
KOiK04093.
OMAiRSAPDCP.
OrthoDBiEOG6TTVS1.

Family and domain databases

Gene3Di1.20.59.10. 1 hit.
InterProiIPR002701. Chorismate_mutase.
IPR008240. Chorismate_mutase_periplasmic.
IPR020822. Chorismate_mutase_type_II.
[Graphical view]
PfamiPF01817. CM_2. 1 hit.
[Graphical view]
PIRSFiPIRSF026640. Peripl_chor_mut. 1 hit.
SMARTiSM00830. CM_2. 1 hit.
[Graphical view]
SUPFAMiSSF48600. SSF48600. 1 hit.
TIGRFAMsiTIGR01806. CM_mono2. 1 hit.
PROSITEiPS51168. CHORISMATE_MUT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0QU81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLASVALAAL AGVGTPHATA DDASPLVPLV DAAAQRLQTA DPVAASKFRS
60 70 80 90 100
GGAIDDPDRE QQVIAAVTGD ATRHNIDPGY VHDVFRNQID ATSSVEHTRF
110 120 130 140 150
AQWKLDPAAA PSSAPDLSES RQKIDTLNRT MVDEIARQWP VLHSPVCRPD
160 170 180
LDRALDAVAT ARGFDPVYRH ALEYATHSYC R
Length:181
Mass (Da):19,491
Last modified:January 9, 2007 - v1
Checksum:i77CD7BECF2CBB014
GO

Sequence cautioni

The sequence AFP38535.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74300.1.
CP001663 Genomic DNA. Translation: AFP38535.1. Different initiation.
RefSeqiWP_011728162.1. NZ_CP009494.1.
YP_886469.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK74300; ABK74300; MSMEG_2111.
AFP38535; AFP38535; MSMEI_2064.
GeneIDi4532159.
KEGGimsb:LJ00_10520.
msg:MSMEI_2064.
msm:MSMEG_2111.
PATRICi18076663. VBIMycSme59918_2087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK74300.1.
CP001663 Genomic DNA. Translation: AFP38535.1. Different initiation.
RefSeqiWP_011728162.1. NZ_CP009494.1.
YP_886469.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QU81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_2111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK74300; ABK74300; MSMEG_2111.
AFP38535; AFP38535; MSMEI_2064.
GeneIDi4532159.
KEGGimsb:LJ00_10520.
msg:MSMEI_2064.
msm:MSMEG_2111.
PATRICi18076663. VBIMycSme59918_2087.

Phylogenomic databases

eggNOGiENOG4105WWF. Bacteria.
COG1605. LUCA.
HOGENOMiHOG000257623.
KOiK04093.
OMAiRSAPDCP.
OrthoDBiEOG6TTVS1.

Enzyme and pathway databases

UniPathwayiUPA00120; UER00203.
BioCyciMSME246196:GJ4Y-2111-MONOMER.
BRENDAi5.4.99.5. 3512.

Family and domain databases

Gene3Di1.20.59.10. 1 hit.
InterProiIPR002701. Chorismate_mutase.
IPR008240. Chorismate_mutase_periplasmic.
IPR020822. Chorismate_mutase_type_II.
[Graphical view]
PfamiPF01817. CM_2. 1 hit.
[Graphical view]
PIRSFiPIRSF026640. Peripl_chor_mut. 1 hit.
SMARTiSM00830. CM_2. 1 hit.
[Graphical view]
SUPFAMiSSF48600. SSF48600. 1 hit.
TIGRFAMsiTIGR01806. CM_mono2. 1 hit.
PROSITEiPS51168. CHORISMATE_MUT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
    Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
    Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
    Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
    Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700084 / mc(2)155.
  4. "The two chorismate mutases from both Mycobacterium tuberculosis and Mycobacterium smegmatis: biochemical analysis and limited regulation of promoter activity by aromatic amino acids."
    Schneider C.Z., Parish T., Basso L.A., Santos D.S.
    J. Bacteriol. 190:122-134(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A CHORISMATE MUTASE.

Entry informationi

Entry nameiSCMU_MYCS2
AccessioniPrimary (citable) accession number: A0QU81
Secondary accession number(s): I7FAF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 9, 2007
Last modified: November 11, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.