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Protein

Diacylglycerol acyltransferase/mycolyltransferase Ag85B

Gene

fbpB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein. They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).By similarity

Catalytic activityi

2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate.
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661NucleophileBy similarity
Binding sitei166 – 1661SubstrateBy similarity
Active sitei272 – 2721By similarity
Active sitei304 – 3041By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2078-MONOMER.

Protein family/group databases

ESTHERimycs2-a0qu51. A85-Mycolyl-transferase.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol acyltransferase/mycolyltransferase Ag85B (EC:2.3.1.122, EC:2.3.1.20)
Short name:
DGAT
Alternative name(s):
30 kDa extracellular protein
Acyl-CoA:diacylglycerol acyltransferase
Antigen 85 complex B
Short name:
85B
Short name:
Ag85B
Extracellular alpha-antigen
Fibronectin-binding protein B
Short name:
Fbps B
Gene namesi
Name:fbpB
Ordered Locus Names:MSMEG_2078, MSMEI_2033
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3838Sequence analysisAdd
BLAST
Chaini39 – 325287Diacylglycerol acyltransferase/mycolyltransferase Ag85BPRO_0000421121Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi127 ↔ 132By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Expression depends on the two-component regulatory system MtrA/MtrB.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_2078.

Structurei

3D structure databases

ProteinModelPortaliA0QU51.
SMRiA0QU51. Positions 41-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate bindingBy similarity
Regioni98 – 10811Fibronectin-bindingBy similarityAdd
BLAST
Regioni274 – 2774Substrate bindingBy similarity
Regioni304 – 3063Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the mycobacterial A85 antigen family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0627. LUCA.
HOGENOMiHOG000049505.
KOiK18851.
OrthoDBiPOG091H0560.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
[Graphical view]
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0QU51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFIDKIRGH WARRMTVAAV AALLLPGLVG VVGGSATAGA FSRPGLPVEY
60 70 80 90 100
LMVPSPSMGR DIKVQFQSGG PGSHAVYLLD GLRAQDDFNG WDINTNAFEM
110 120 130 140 150
FLDSGLSVVM PVGGQSSFYS DWYQPACGNN GCVTYKWETF LTSELPEWLA
160 170 180 190 200
ANRDVAATGN AAIGLSMAGS AALILAAYHP DRFIYAGSMS GFLNPSEGWW
210 220 230 240 250
PFLINISMGD AGGYKANDMW GPTEDPNSAW KRNDPMVQIP RLVANNTRIW
260 270 280 290 300
VYCGNGQPNE LGGGDLPATF LEGLTIRTNE TFRDNYIAAG GNNGVFNFPN
310 320
NGTHNWAYWG RELQAMVPDL QRVLG
Length:325
Mass (Da):35,190
Last modified:January 9, 2007 - v1
Checksum:iD0F4EA32E445270B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75892.1.
CP001663 Genomic DNA. Translation: AFP38504.1.
RefSeqiWP_003893447.1. NZ_CP009494.1.
YP_886439.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK75892; ABK75892; MSMEG_2078.
AFP38504; AFP38504; MSMEI_2033.
GeneIDi4533257.
KEGGimsb:LJ00_10360.
msg:MSMEI_2033.
msm:MSMEG_2078.
PATRICi18076597. VBIMycSme59918_2054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75892.1.
CP001663 Genomic DNA. Translation: AFP38504.1.
RefSeqiWP_003893447.1. NZ_CP009494.1.
YP_886439.1. NC_008596.1.

3D structure databases

ProteinModelPortaliA0QU51.
SMRiA0QU51. Positions 41-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_2078.

Protein family/group databases

ESTHERimycs2-a0qu51. A85-Mycolyl-transferase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK75892; ABK75892; MSMEG_2078.
AFP38504; AFP38504; MSMEI_2033.
GeneIDi4533257.
KEGGimsb:LJ00_10360.
msg:MSMEI_2033.
msm:MSMEG_2078.
PATRICi18076597. VBIMycSme59918_2054.

Phylogenomic databases

eggNOGiCOG0627. LUCA.
HOGENOMiHOG000049505.
KOiK18851.
OrthoDBiPOG091H0560.

Enzyme and pathway databases

BioCyciMSME246196:GJ4Y-2078-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
[Graphical view]
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA85B_MYCS2
AccessioniPrimary (citable) accession number: A0QU51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.