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Protein

Sensor histidine kinase MtrB

Gene

mtrB

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system MtrA/MtrB. Probably functions as a membrane-associated protein kinase that phosphorylates MtrA in response to environmental signals (By similarity). MtrB may be active only at septa where it promotes MtrA phosphorylation and MtrA regulon expression, probably playing a role in cell division.By similarity1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase MtrB (EC:2.7.13.3)
Gene namesi
Name:mtrB
Ordered Locus Names:MSMEG_1875, MSMEI_1836
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei42 – 62HelicalSequence analysisAdd BLAST21
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Grows poorly with filamentous cells, often with bulbous regions and a chain-like phenotype indicating possible defects in septum splitting and cell wall metabolism. Increased sensitivity to lysozyme. Decreased expression of the MtrA/MtrB regulon genes dnaA, ripA, and fbpB.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004211231 – 553Sensor histidine kinase MtrBAdd BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei304Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with MrtA.Curated

Protein-protein interaction databases

STRINGi246196.MSMEG_1875.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini234 – 286HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini301 – 518Histidine kinasePROSITE-ProRule annotationAdd BLAST218

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000247709.
KOiK07654.
OrthoDBiPOG091H0351.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0QTK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFGSRRRIR GRWGGSGPLV RGLGTLGRAL SLVWRRSLQL RVVTLTLGLS
60 70 80 90 100
LAVILVLGFV LTSQITDRIL EVKVKAATEE VERARNTVSG IVGGEESRSL
110 120 130 140 150
ESSLQLARNT LVDRKADVRA DMAGAFDAVL VVPGDGPRAA AAAGPVAQIP
160 170 180 190 200
EALRDFVKAG QVSYQYATVQ TEGFSGPALI VGSPTTSSVP NLELYLIFPL
210 220 230 240 250
NNEESTIALV RGTMATGGVV LLGLLAAIAL VVARQIVQPV RSASRIAERF
260 270 280 290 300
AEGHLTERMP VRGEDDMARL AVSFNDMAES LSRQIQQLEE FGNLQRRFTS
310 320 330 340 350
DVSHELRTPL TTVRMAADLI YDHSEDLDPA LRRSTELMVN ELDRFETLLA
360 370 380 390 400
DLLEISRHDA GVAELSVESL DLRSTVRSAL DNVGHLAADA GVELTLDMPE
410 420 430 440 450
EDVIAEVDPR RVERILRNLI ANAIDHAESK PVQIRMAADE DTVAVTVRDF
460 470 480 490 500
GVGLRPGEEK LVFSRFWRSD PSRVRRSGGT GLGLAISIED ARLHQGRLEA
510 520 530 540 550
WGEPGKGACF RLTLPLVRGH KVTTSPLPLK PASEQRPARR RSNKDREAAE

ESV
Length:553
Mass (Da):60,233
Last modified:February 6, 2013 - v2
Checksum:i4EF6F34D5571C136
GO

Sequence cautioni

The sequence ABK75150 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75150.1. Different initiation.
CP001663 Genomic DNA. Translation: AFP38308.1.
RefSeqiWP_011727972.1. NC_008596.1.
WP_014877234.1. NZ_CP009494.1.
YP_886241.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK75150; ABK75150; MSMEG_1875.
AFP38308; AFP38308; MSMEI_1836.
GeneIDi4532306.
KEGGimsb:LJ00_09360.
msg:MSMEI_1836.
msm:MSMEG_1875.
PATRICi18076201. VBIMycSme59918_1857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA. Translation: ABK75150.1. Different initiation.
CP001663 Genomic DNA. Translation: AFP38308.1.
RefSeqiWP_011727972.1. NC_008596.1.
WP_014877234.1. NZ_CP009494.1.
YP_886241.1. NC_008596.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_1875.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK75150; ABK75150; MSMEG_1875.
AFP38308; AFP38308; MSMEI_1836.
GeneIDi4532306.
KEGGimsb:LJ00_09360.
msg:MSMEI_1836.
msm:MSMEG_1875.
PATRICi18076201. VBIMycSme59918_1857.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000247709.
KOiK07654.
OrthoDBiPOG091H0351.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTRB_MYCS2
AccessioniPrimary (citable) accession number: A0QTK3
Secondary accession number(s): I7FHM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: February 6, 2013
Last modified: November 2, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.