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Protein

dTDP-4-dehydrorhamnose reductase

Gene

rmlD

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage.1 Publication

Catalytic activityi

dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

Pathwayi: dTDP-L-rhamnose biosynthesis

This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei182Substrate; via amide nitrogenBy similarity1
Binding sitei183NAD; via amide nitrogenBy similarity1
Binding sitei281SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 44NADBy similarity5
Nucleotide bindingi44 – 45NADPBy similarity2
Nucleotide bindingi63 – 64NADBy similarity2
Nucleotide bindingi69 – 70NAD/NADPBy similarity2
Nucleotide bindingi90 – 93NADBy similarity4
Nucleotide bindingi91 – 93NADPBy similarity3
Nucleotide bindingi157 – 161NAD/NADPBy similarity5

GO - Molecular functioni

  • dTDP-4-dehydrorhamnose reductase activity Source: UniProtKB

GO - Biological processi

  • dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
  • extracellular polysaccharide biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00124

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-4-dehydrorhamnose reductase (EC:1.1.1.133)
Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase
Gene namesi
Name:rmlD
Synonyms:rfbD
Ordered Locus Names:MSMEG_1825, MSMEI_1782
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003999001 – 327dTDP-4-dehydrorhamnose reductaseAdd BLAST327

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_1825

Structurei

3D structure databases

ProteinModelPortaliA0QTF8
SMRiA0QTF8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 133Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DBZ Bacteria
COG1091 LUCA
HOGENOMiHOG000227711
KOiK00067
OMAiYHYSNEG
OrthoDBiPOG091H07IL

Family and domain databases

InterProiView protein in InterPro
IPR005913 dTDP_dehydrorham_reduct
IPR036291 NAD(P)-bd_dom_sf
IPR029903 RmlD-like-bd
PANTHERiPTHR10491 PTHR10491, 1 hit
PfamiView protein in Pfam
PF04321 RmlD_sub_bind, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01214 rmlD, 1 hit

Sequencei

Sequence statusi: Complete.

A0QTF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLINGMGTS PGYWRTPREP GNDHRRARLD VMAQRIVITG AGGMVGRVLA
60 70 80 90 100
DQAAAKGHTV LALTSSQCDI TDEDAVRRFV ANGDVVINCA AYTQVDKAED
110 120 130 140 150
EPERAHAVNA VGPGNLAKAC AAVDAGLIHI STDYVFGAVD RDTPYEVDDE
160 170 180 190 200
TGPVNIYGRT KLAGEQAVLA AKPDAYVVRT AWVYRGGDGS DFVATMRRLA
210 220 230 240 250
AGDGAIDVVA DQVGSPTYTG DLVGALLQIV DGGVEPGILH AANAGVASRF
260 270 280 290 300
DQARATFEAV GADPERVRPC GSDRHPRPAP RPSYTVLSSQ RSAQAGLTPL
310 320
RDWREALQDA VAAVVGATTD GPLPSTP
Length:327
Mass (Da):34,254
Last modified:January 9, 2007 - v1
Checksum:i5AD5A5F7337A7276
GO

Sequence cautioni

The sequence AFP38254 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000480 Genomic DNA Translation: ABK74972.1
CP001663 Genomic DNA Translation: AFP38254.1 Different initiation.
RefSeqiWP_011727934.1, NZ_CP009494.1
YP_886196.1, NC_008596.1

Genome annotation databases

EnsemblBacteriaiABK74972; ABK74972; MSMEG_1825
AFP38254; AFP38254; MSMEI_1782
GeneIDi4533132
KEGGimsg:MSMEI_1782
msm:MSMEG_1825
PATRICifig|246196.19.peg.1807

Entry informationi

Entry nameiRMLD_MYCS2
AccessioniPrimary (citable) accession number: A0QTF8
Secondary accession number(s): I7G6I9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: January 9, 2007
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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