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A0QTF8

- RMLD_MYCS2

UniProt

A0QTF8 - RMLD_MYCS2

Protein

dTDP-4-dehydrorhamnose reductase

Gene

rmlD

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
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    Functioni

    Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage.1 Publication

    Catalytic activityi

    dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei182 – 1821Substrate; via amide nitrogenBy similarity
    Binding sitei183 – 1831NAD; via amide nitrogenBy similarity
    Binding sitei281 – 2811SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi40 – 445NADBy similarity
    Nucleotide bindingi44 – 452NADPBy similarity
    Nucleotide bindingi63 – 642NADBy similarity
    Nucleotide bindingi69 – 702NAD/NADPBy similarity
    Nucleotide bindingi90 – 934NADBy similarity
    Nucleotide bindingi91 – 933NADPBy similarity
    Nucleotide bindingi157 – 1615NAD/NADPBy similarity

    GO - Molecular functioni

    1. dTDP-4-dehydrorhamnose reductase activity Source: UniProtKB

    GO - Biological processi

    1. dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
    2. extracellular polysaccharide biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-1825-MONOMER.
    UniPathwayiUPA00124.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    dTDP-4-dehydrorhamnose reductase (EC:1.1.1.133)
    Alternative name(s):
    dTDP-4-keto-L-rhamnose reductase
    dTDP-6-deoxy-L-lyxo-4-hexulose reductase
    dTDP-6-deoxy-L-mannose dehydrogenase
    dTDP-L-rhamnose synthase
    Gene namesi
    Name:rmlD
    Synonyms:rfbD
    Ordered Locus Names:MSMEG_1825, MSMEI_1782
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327dTDP-4-dehydrorhamnose reductasePRO_0000399900Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi246196.MSMEG_1825.

    Structurei

    3D structure databases

    ProteinModelPortaliA0QTF8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni132 – 1332Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1091.
    HOGENOMiHOG000227711.
    KOiK00067.
    OMAiLTNAGSP.
    OrthoDBiEOG6HTP2V.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR005913. dTDP_dehydrorham_reduct.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF04321. RmlD_sub_bind. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01214. rmlD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0QTF8-1 [UniParc]FASTAAdd to Basket

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    MDLINGMGTS PGYWRTPREP GNDHRRARLD VMAQRIVITG AGGMVGRVLA    50
    DQAAAKGHTV LALTSSQCDI TDEDAVRRFV ANGDVVINCA AYTQVDKAED 100
    EPERAHAVNA VGPGNLAKAC AAVDAGLIHI STDYVFGAVD RDTPYEVDDE 150
    TGPVNIYGRT KLAGEQAVLA AKPDAYVVRT AWVYRGGDGS DFVATMRRLA 200
    AGDGAIDVVA DQVGSPTYTG DLVGALLQIV DGGVEPGILH AANAGVASRF 250
    DQARATFEAV GADPERVRPC GSDRHPRPAP RPSYTVLSSQ RSAQAGLTPL 300
    RDWREALQDA VAAVVGATTD GPLPSTP 327
    Length:327
    Mass (Da):34,254
    Last modified:January 9, 2007 - v1
    Checksum:i5AD5A5F7337A7276
    GO

    Sequence cautioni

    The sequence AFP38254.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK74972.1.
    CP001663 Genomic DNA. Translation: AFP38254.1. Different initiation.
    RefSeqiYP_006566549.1. NC_018289.1.
    YP_886196.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK74972; ABK74972; MSMEG_1825.
    AFP38254; AFP38254; MSMEI_1782.
    GeneIDi4533132.
    KEGGimsg:MSMEI_1782.
    msm:MSMEG_1825.
    PATRICi18076099. VBIMycSme59918_1807.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000480 Genomic DNA. Translation: ABK74972.1 .
    CP001663 Genomic DNA. Translation: AFP38254.1 . Different initiation.
    RefSeqi YP_006566549.1. NC_018289.1.
    YP_886196.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali A0QTF8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_1825.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK74972 ; ABK74972 ; MSMEG_1825 .
    AFP38254 ; AFP38254 ; MSMEI_1782 .
    GeneIDi 4533132.
    KEGGi msg:MSMEI_1782.
    msm:MSMEG_1825.
    PATRICi 18076099. VBIMycSme59918_1807.

    Phylogenomic databases

    eggNOGi COG1091.
    HOGENOMi HOG000227711.
    KOi K00067.
    OMAi LTNAGSP.
    OrthoDBi EOG6HTP2V.

    Enzyme and pathway databases

    UniPathwayi UPA00124 .
    BioCyci MSME246196:GJ4Y-1825-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR005913. dTDP_dehydrorham_reduct.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF04321. RmlD_sub_bind. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01214. rmlD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    2. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Formation of dTDP-rhamnose is essential for growth of mycobacteria."
      Ma Y., Pan F., McNeil M.
      J. Bacteriol. 184:3392-3395(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DTDP-RHAMNOSE BIOSYNTHESIS.

    Entry informationi

    Entry nameiRMLD_MYCS2
    AccessioniPrimary (citable) accession number: A0QTF8
    Secondary accession number(s): I7G6I9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3