A0QTF8 (RMLD_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
dTDP-4-dehydrorhamnose reductase
EC=1.1.1.133

Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase

Gene names

Name:	rmlD
Synonyms:	rfbD
Ordered Locus Names:	MSMEG_1825, MSMEI_1782

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) [Reference proteome] [HAMAP]

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium smegmatis

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage. Ref.4
Catalytic activity	dTDP-6-deoxy-beta-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-beta-L-mannose + NADPH.
Pathway	Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.
Sequence similarities	Belongs to the dTDP-4-dehydrorhamnose reductase family.
Sequence caution	The sequence AFP38254.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTDP-rhamnose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway extracellular polysaccharide biosynthetic process Inferred from genetic interaction Ref.4. Source: UniProtKB
Molecular_function	dTDP-4-dehydrorhamnose reductase activity Inferred from genetic interaction Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	dTDP-4-dehydrorhamnose reductase		PRO_0000399900
Regions
	Nucleotide binding	40 – 44	5	NAD By similarity
	Nucleotide binding	44 – 45	2	NADP By similarity
	Nucleotide binding	63 – 64	2	NAD By similarity
	Nucleotide binding	69 – 70	2	NAD/NADP By similarity
	Nucleotide binding	90 – 93	4	NAD By similarity
	Nucleotide binding	91 – 93	3	NADP By similarity
	Nucleotide binding	157 – 161	5	NAD/NADP By similarity
	Region	132 – 133	2	Substrate binding By similarity
Sites
	Binding site	182	1	Substrate; via amide nitrogen By similarity
	Binding site	183	1	NAD; via amide nitrogen By similarity
	Binding site	281	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0QTF8 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 5AD5A5F7337A7276	FASTA	327	34,254
	10 20 30 40 50 60 MDLINGMGTS PGYWRTPREP GNDHRRARLD VMAQRIVITG AGGMVGRVLA DQAAAKGHTV 70 80 90 100 110 120 LALTSSQCDI TDEDAVRRFV ANGDVVINCA AYTQVDKAED EPERAHAVNA VGPGNLAKAC 130 140 150 160 170 180 AAVDAGLIHI STDYVFGAVD RDTPYEVDDE TGPVNIYGRT KLAGEQAVLA AKPDAYVVRT 190 200 210 220 230 240 AWVYRGGDGS DFVATMRRLA AGDGAIDVVA DQVGSPTYTG DLVGALLQIV DGGVEPGILH 250 260 270 280 290 300 AANAGVASRF DQARATFEAV GADPERVRPC GSDRHPRPAP RPSYTVLSSQ RSAQAGLTPL 310 320 RDWREALQDA VAAVVGATTD GPLPSTP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[2]	"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?" Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M. Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[3]	"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol." Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O. Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.
[4]	"Formation of dTDP-rhamnose is essential for growth of mycobacteria." Ma Y., Pan F., McNeil M. J. Bacteriol. 184:3392-3395(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN DTDP-RHAMNOSE BIOSYNTHESIS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000480 Genomic DNA. Translation: ABK74972.1. CP001663 Genomic DNA. Translation: AFP38254.1. Different initiation.
RefSeq	YP_006566549.1. NC_018289.1. YP_886196.1. NC_008596.1.
3D structure databases
ProteinModelPortal	A0QTF8.
ModBase	Search...
Protein-protein interaction databases
STRING	246196.MSMEG_1825.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK74972; ABK74972; MSMEG_1825.
GeneID	13429595. 4533132.
KEGG	msg:MSMEI_1782. msm:MSMEG_1825.
PATRIC	18076099. VBIMycSme59918_1807.
Phylogenomic databases
eggNOG	COG1091.
HOGENOM	HOG000227711.
KO	K00067.
OMA	GSPTYVG.
Enzyme and pathway databases
BioCyc	MSME246196:GJ4Y-1825-MONOMER.
UniPathway	UPA00124.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR005913. dTDP_dehydrorham_reduct. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF04321. RmlD_sub_bind. 1 hit. [Graphical view]
TIGRFAMs	TIGR01214. rmlD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RMLD_MYCS2

Accession

Primary (citable) accession number: A0QTF8
Secondary accession number(s): I7G6I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
Last sequence update:	January 9, 2007
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program